2-AG promotes the expression of conditioned fear via
cannabinoid receptor type 1 on GABAergic neurons

J 2015

2-AG promotes the expression of conditioned fear via cannabinoid receptor type 1 on GABAergic neurons

LLORENTE-BERZAL, Alvaro; Ana Luisa B. TERZIAN; Vincenzo DI MARZO; Vincenzo MICALE; Maria Paz VIVEROS et al.

Základní údaje

Originální název

2-AG promotes the expression of conditioned fear via cannabinoid receptor type 1 on GABAergic neurons

Autoři

LLORENTE-BERZAL, Alvaro; Ana Luisa B. TERZIAN; Vincenzo DI MARZO; Vincenzo MICALE; Maria Paz VIVEROS a Carsten T. WOTJAK

Vydání

Psychopharmacology, New York, Springer, 2015, 0033-3158

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30000 3. Medical and Health Sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.540

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14740/15:00084725

Organizační jednotka

Středoevropský technologický institut

Klíčová slova anglicky

Fear extinction; TRPV1; CB1; CB2; URB597; JZL184; AM404; Anandamide

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 1. 4. 2016 15:59, Mgr. Eva Špillingová

Anotace

V originále

The contribution of two major endocannabinoids, 2-arachidonoylglycerol (2-AG) and anandamide (AEA), in the regulation of fear expression is still unknown. We analyzed the role of different players of the endocannabinoid system on the expression of a strong auditory-cued fear memory in male mice by pharmacological means. The cannabinoid receptor type 1 (CB1) antagonist SR141716 (3 mg/kg) caused an increase in conditioned freezing upon repeated tone presentation on three consecutive days. The cannabinoid receptor type 2 (CB2) antagonist AM630 (3 mg/kg), in contrast, had opposite effects during the first tone presentation, with no effects of the transient receptor potential vanilloid receptor type 1 (TRPV1) antagonist SB366791 (1 and 3 mg/kg). Administration of the CB2 agonist JWH133 (3 mg/kg) failed to affect the acute freezing response, whereas the CB1 agonist CP55,940 (50 mu g/kg) augmented it. The endocannabinoid uptake inhibitor AM404 (3 mg/kg), but not VDM11 (3 mg/kg), reduced the acute freezing response. Its co-administration with SR141716 or SB366791 confirmed an involvement of CB1 and TRPV1. AEA degradation inhibition by URB597 (1 mg/kg) decreased, while 2-AG degradation inhibition by JZL184 (4 and 8 mg/kg) increased freezing response. As revealed in conditional CB1-deficient mutants, CB1 on cortical glutamatergic neurons alleviates whereas CB1 on GABAergic neurons slightly enhances fear expression. Moreover, 2-AG fear-promoting effects depended on CB1 signaling in GABAergic neurons, while an involvement of glutamatergic neurons remained inconclusive due to the high freezing shown by vehicle-treated Glu-CB1-KO. Our findings suggest that increased AEA levels mediate acute fear relief, whereas increased 2-AG levels promote the expression of conditioned fear primarily via CB1 on GABAergic neurons.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

Citovat

LLORENTE-BERZAL, Alvaro; Ana Luisa B. TERZIAN; Vincenzo DI MARZO; Vincenzo MICALE; Maria Paz VIVEROS a Carsten T. WOTJAK. 2-AG promotes the expression of conditioned fear via cannabinoid receptor type 1 on GABAergic neurons. Psychopharmacology. New York: Springer, 2015, roč. 232, č. 15, s. 2811-2825. ISSN 0033-3158. Dostupné z: https://doi.org/10.1007/s00213-015-3917-y.

@article{1317673,
   author = {LlorenteandBerzal, Alvaro and Terzian, Ana Luisa B. and di Marzo, Vincenzo and Micale, Vincenzo and Viveros, Maria Paz and Wotjak, Carsten T.},
   article_location = {New York},
   article_number = {15},
   doi = {https://doi.org/10.1007/s00213-015-3917-y},
   keywords = {Fear extinction; TRPV1; CB1; CB2; URB597; JZL184; AM404; Anandamide},
   language = {eng},
   issn = {0033-3158},
   journal = {Psychopharmacology},
   title = {2-AG promotes the expression of conditioned fear via cannabinoid receptor type 1 on GABAergic neurons},
   url = {http://download.springer.com/static/pdf/322/art%253A10.1007%252Fs00213-015-3917-y.pdf?originUrl=http%3A%2F%2Flink.springer.com%2Farticle%2F10.1007%2Fs00213-015-3917-y&token2=exp=1447855112~acl=%2Fstatic%2Fpdf%2F322%2Fart%25253A10.1007%25252Fs00213-015-39},
   volume = {232},
   year = {2015}
}

TY  - JOUR
ID  - 1317673
AU  - Llorente-Berzal, Alvaro - Terzian, Ana Luisa B. - di Marzo, Vincenzo - Micale, Vincenzo - Viveros, Maria Paz - Wotjak, Carsten T.
PY  - 2015
TI  - 2-AG promotes the expression of conditioned fear via cannabinoid receptor type 1 on GABAergic neurons
JF  - Psychopharmacology
VL  - 232
IS  - 15
SP  - 2811-2825
EP  - 2811-2825
PB  - Springer
SN  - 00333158
KW  - Fear extinction
KW  - TRPV1
KW  - CB1
KW  - CB2
KW  - URB597
KW  - JZL184
KW  - AM404
KW  - Anandamide
UR  - http://download.springer.com/static/pdf/322/art%253A10.1007%252Fs00213-015-3917-y.pdf?originUrl=http%3A%2F%2Flink.springer.com%2Farticle%2F10.1007%2Fs00213-015-3917-y&token2=exp=1447855112~acl=%2Fstatic%2Fpdf%2F322%2Fart%25253A10.1007%25252Fs00213-015-39
L2  - http://download.springer.com/static/pdf/322/art%253A10.1007%252Fs00213-015-3917-y.pdf?originUrl=http%3A%2F%2Flink.springer.com%2Farticle%2F10.1007%2Fs00213-015-3917-y&token2=exp=1447855112~acl=%2Fstatic%2Fpdf%2F322%2Fart%25253A10.1007%25252Fs00213-015-39
N2  - The contribution of two major endocannabinoids, 2-arachidonoylglycerol (2-AG) and anandamide (AEA), in the regulation of fear expression is still unknown. We analyzed the role of different players of the endocannabinoid system on the expression of a strong auditory-cued fear memory in male mice by pharmacological means. The cannabinoid receptor type 1 (CB1) antagonist SR141716 (3 mg/kg) caused an increase in conditioned freezing upon repeated tone presentation on three consecutive days. The cannabinoid receptor type 2 (CB2) antagonist AM630 (3 mg/kg), in contrast, had opposite effects during the first tone presentation, with no effects of the transient receptor potential vanilloid receptor type 1 (TRPV1) antagonist SB366791 (1 and 3 mg/kg). Administration of the CB2 agonist JWH133 (3 mg/kg) failed to affect the acute freezing response, whereas the CB1 agonist CP55,940 (50 mu g/kg) augmented it. The endocannabinoid uptake inhibitor AM404 (3 mg/kg), but not VDM11 (3 mg/kg), reduced the acute freezing response. Its co-administration with SR141716 or SB366791 confirmed an involvement of CB1 and TRPV1. AEA degradation inhibition by URB597 (1 mg/kg) decreased, while 2-AG degradation inhibition by JZL184 (4 and 8 mg/kg) increased freezing response. As revealed in conditional CB1-deficient mutants, CB1 on cortical glutamatergic neurons alleviates whereas CB1 on GABAergic neurons slightly enhances fear expression. Moreover, 2-AG fear-promoting effects depended on CB1 signaling in GABAergic neurons, while an involvement of glutamatergic neurons remained inconclusive due to the high freezing shown by vehicle-treated Glu-CB1-KO. Our findings suggest that increased AEA levels mediate acute fear relief, whereas increased 2-AG levels promote the expression of conditioned fear primarily via CB1 on GABAergic neurons.
ER  -

LLORENTE-BERZAL, Alvaro; Ana Luisa B. TERZIAN; Vincenzo DI MARZO; Vincenzo MICALE; Maria Paz VIVEROS a Carsten T. WOTJAK. 2-AG promotes the expression of conditioned fear via cannabinoid receptor type 1 on GABAergic neurons. \textit{Psychopharmacology}. New York: Springer, 2015, roč.~232, č.~15, s.~2811-2825. ISSN~0033-3158. Dostupné z: https://doi.org/10.1007/s00213-015-3917-y.

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