Toward Structured Macroporous Hydrogel Composites: Electron
Beam-Initiated Polymerization of Layered Cryogels

J 2015

Toward Structured Macroporous Hydrogel Composites: Electron Beam-Initiated Polymerization of Layered Cryogels

GOLUNOVA, Anna; David CHVÁTIL; Pavel KRIST; Josef JAROŠ; Veronika JURTÍKOVÁ et al.

Základní údaje

Originální název

Toward Structured Macroporous Hydrogel Composites: Electron Beam-Initiated Polymerization of Layered Cryogels

Autoři

Vydání

Biomacromolecules, Washington, American Chemical Society, 2015, 1525-7797

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 5.583

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/15:00084751

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

STEM-CELLS; POLYSACCHARIDE HYDROGELS; MECHANICAL-PROPERTIES; EXTRACELLULAR-MATRIX; TISSUE REGENERATION; AQUEOUS-SOLUTIONS; ADIPOSE-TISSUE; DRUG-DELIVERY; DIFFERENTIATION; SCAFFOLDS

Štítky

EL OK

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 19. 11. 2015 12:22, Ing. Mgr. Věra Pospíšilíková

Anotace

V originále

The ability to tailor mechanical properties and architecture is crucial in creating macroporous hydrogel scaffolds for tissue engineering. In the present work, a technique for the modification of the pore size and stiffness of acrylamide-based cryogels is demonstrated via the regulation of an electron beam irradiation dose. The samples were characterized by equilibrium swelling measurements, light and scanning electron microscopy, mercury porosimetry, Brunauer-Emmett-Teller surface area analysis, and stiffness measurements. Their properties were compared to cryogels prepared by a standard redox-initiated radical polymerization. A I-125 radiolabeled azidopentanoyl-GGGRGDSGGGY-NH2 peptide was bound to the surface to determine the concentration of the adhesive sites available for biomimetic modification. The functionality of the prepared substrates was evaluated by in vitro cultivation of adipose-derived stem cells. Moreover, the feasibility of preparing layered cryogels was demonstrated. This may be the key to the future preparation of complex hydrogel-based scaffolds to mimic the extracellular microenvironment in a wide range of applications.

Návaznosti

EE2.3.20.0185, projekt VaV

Název: Centrum analýz a modelování tkání a orgánů

Citovat

GOLUNOVA, Anna; David CHVÁTIL; Pavel KRIST; Josef JAROŠ; Veronika JURTÍKOVÁ; Jakub POSPÍŠIL; Ilya KOTELNIKOV; Lucie ABELOVÁ; Jiří KOTEK; Tomáš SEDLAČÍK; Jan KUČKA; Jana KOUBKOVÁ; Hana STUDENOVSKÁ; Libor STREIT; Aleš HAMPL; František RYPÁČEK a Vladimír PROKS. Toward Structured Macroporous Hydrogel Composites: Electron Beam-Initiated Polymerization of Layered Cryogels. Biomacromolecules. Washington: American Chemical Society, 2015, roč. 16, č. 4, s. 1146-1156. ISSN 1525-7797. Dostupné z: https://doi.org/10.1021/bm501809t.

@article{1317739,
   author = {Golunova, Anna and Chvátil, David and Krist, Pavel and Jaroš, Josef and Jurtíková, Veronika and Pospíšil, Jakub and Kotelnikov, Ilya and Abelová, Lucie and Kotek, Jiří and Sedlačík, Tomáš and Kučka, Jan and Koubková, Jana and Studenovská, Hana and Streit, Libor and Hampl, Aleš and Rypáček, František and Proks, Vladimír},
   article_location = {Washington},
   article_number = {4},
   doi = {https://doi.org/10.1021/bm501809t},
   keywords = {STEM-CELLS; POLYSACCHARIDE HYDROGELS; MECHANICAL-PROPERTIES; EXTRACELLULAR-MATRIX; TISSUE REGENERATION; AQUEOUS-SOLUTIONS; ADIPOSE-TISSUE; DRUG-DELIVERY; DIFFERENTIATION; SCAFFOLDS},
   language = {eng},
   issn = {1525-7797},
   journal = {Biomacromolecules},
   title = {Toward Structured Macroporous Hydrogel Composites: Electron Beam-Initiated Polymerization of Layered Cryogels},
   volume = {16},
   year = {2015}
}

TY  - JOUR
ID  - 1317739
AU  - Golunova, Anna - Chvátil, David - Krist, Pavel - Jaroš, Josef - Jurtíková, Veronika - Pospíšil, Jakub - Kotelnikov, Ilya - Abelová, Lucie - Kotek, Jiří - Sedlačík, Tomáš - Kučka, Jan - Koubková, Jana - Studenovská, Hana - Streit, Libor - Hampl, Aleš - Rypáček, František - Proks, Vladimír
PY  - 2015
TI  - Toward Structured Macroporous Hydrogel Composites: Electron Beam-Initiated Polymerization of Layered Cryogels
JF  - Biomacromolecules
VL  - 16
IS  - 4
SP  - 1146-1156
EP  - 1146-1156
PB  - American Chemical Society
SN  - 15257797
KW  - STEM-CELLS
KW  - POLYSACCHARIDE HYDROGELS
KW  - MECHANICAL-PROPERTIES
KW  - EXTRACELLULAR-MATRIX
KW  - TISSUE REGENERATION
KW  - AQUEOUS-SOLUTIONS
KW  - ADIPOSE-TISSUE
KW  - DRUG-DELIVERY
KW  - DIFFERENTIATION
KW  - SCAFFOLDS
N2  - The ability to tailor mechanical properties and architecture is crucial in creating macroporous hydrogel scaffolds for tissue engineering. In the present work, a technique for the modification of the pore size and stiffness of acrylamide-based cryogels is demonstrated via the regulation of an electron beam irradiation dose. The samples were characterized by equilibrium swelling measurements, light and scanning electron microscopy, mercury porosimetry, Brunauer-Emmett-Teller surface area analysis, and stiffness measurements. Their properties were compared to cryogels prepared by a standard redox-initiated radical polymerization. A I-125 radiolabeled azidopentanoyl-GGGRGDSGGGY-NH2 peptide was bound to the surface to determine the concentration of the adhesive sites available for biomimetic modification. The functionality of the prepared substrates was evaluated by in vitro cultivation of adipose-derived stem cells. Moreover, the feasibility of preparing layered cryogels was demonstrated. This may be the key to the future preparation of complex hydrogel-based scaffolds to mimic the extracellular microenvironment in a wide range of applications.
ER  -

GOLUNOVA, Anna; David CHVÁTIL; Pavel KRIST; Josef JAROŠ; Veronika JURTÍKOVÁ; Jakub POSPÍŠIL; Ilya KOTELNIKOV; Lucie ABELOVÁ; Jiří KOTEK; Tomáš SEDLAČÍK; Jan KUČKA; Jana KOUBKOVÁ; Hana STUDENOVSKÁ; Libor STREIT; Aleš HAMPL; František RYPÁČEK a Vladimír PROKS. Toward Structured Macroporous Hydrogel Composites: Electron Beam-Initiated Polymerization of Layered Cryogels. \textit{Biomacromolecules}. Washington: American Chemical Society, 2015, roč.~16, č.~4, s.~1146-1156. ISSN~1525-7797. Dostupné z: https://doi.org/10.1021/bm501809t.

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