N-(2-Hydroxypropyl) Methacrylamide Based Cryogels – Synthesis
and Biomimetic Modification for Stem Cell Applications

J 2015

N-(2-Hydroxypropyl) Methacrylamide Based Cryogels – Synthesis and Biomimetic Modification for Stem Cell Applications

GOLUNOVA, A.; Josef JAROŠ; Veronika JURTÍKOVÁ; I. KOTELNIKOV; J. KOTEK et al.

Základní údaje

Originální název

N-(2-Hydroxypropyl) Methacrylamide Based Cryogels – Synthesis and Biomimetic Modification for Stem Cell Applications

Autoři

GOLUNOVA, A.; Josef JAROŠ; Veronika JURTÍKOVÁ; I. KOTELNIKOV; J. KOTEK; H. HLÍDKOVÁ; Libor STREIT; Aleš HAMPL; F. RYPÁČEK a V. PROKS

Vydání

Physiological Research, Praha, Fyziologický ústav AV ČR, 2015, 0862-8408

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.643

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/15:00084753

Organizační jednotka

Lékařská fakulta

UT WoS

000365010700004

Klíčová slova anglicky

Hydrogels; Cryogels; Stem cells; Scaffolds; Mechanical properties

Štítky

EL OK

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 7. 4. 2016 16:16, Ing. Mgr. Věra Pospíšilíková

Anotace

V originále

The design of favorable mechanical properties and suitable surface modifications of hydrogels in order to stimulate specific cell response is a great challenge. N-(2-Hydroxypropyl) methacryl-amide (HPMA) was utilized to form macroporous cryogel scaffolds for stem cell applications. Furthermore, one group of scaffolds was enhanced by copolymerization of HPMA with methacryloyl-GGGRGDS-OH peptide in an effort to integrate biomimetic adhesion sites. The cryogels were characterized by stiffness and equilibrium swelling measurements as well as by scanning electron microscopy. Cell culture experiments were performed with human adipose-derived stem cells and substrates were found completely non-toxic. Moreover, RGDS-enriched cryogels supported cell attachment, spreading and proliferation, so they can be considered suitable for designed aims.

Návaznosti

EE2.3.20.0185, projekt VaV

Název: Centrum analýz a modelování tkání a orgánů

MUNI/A/1558/2014, interní kód MU

Název: Zdroje pro tkáňové inženýrství 5 (Akronym: TissueEng5)

Investor: Masarykova univerzita, Zdroje pro tkáňové inženýrství 5, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

GOLUNOVA, A.; Josef JAROŠ; Veronika JURTÍKOVÁ; I. KOTELNIKOV; J. KOTEK; H. HLÍDKOVÁ; Libor STREIT; Aleš HAMPL; F. RYPÁČEK a V. PROKS. N-(2-Hydroxypropyl) Methacrylamide Based Cryogels – Synthesis and Biomimetic Modification for Stem Cell Applications. Physiological Research. Praha: Fyziologický ústav AV ČR, 2015, roč. 64, Suppl. 1, s. "S19"-"S27", 9 s. ISSN 0862-8408.

@article{1317742,
   author = {Golunova, A. and Jaroš, Josef and Jurtíková, Veronika and Kotelnikov, I. and Kotek, J. and Hlídková, H. and Streit, Libor and Hampl, Aleš and Rypáček, F. and Proks, V.},
   article_location = {Praha},
   article_number = {Suppl. 1},
   keywords = {Hydrogels; Cryogels; Stem cells; Scaffolds; Mechanical properties},
   language = {eng},
   issn = {0862-8408},
   journal = {Physiological Research},
   title = {N-(2-Hydroxypropyl) Methacrylamide Based Cryogels – Synthesis and Biomimetic Modification for Stem Cell Applications},
   volume = {64},
   year = {2015}
}

TY  - JOUR
ID  - 1317742
AU  - Golunova, A. - Jaroš, Josef - Jurtíková, Veronika - Kotelnikov, I. - Kotek, J. - Hlídková, H. - Streit, Libor - Hampl, Aleš - Rypáček, F. - Proks, V.
PY  - 2015
TI  - N-(2-Hydroxypropyl) Methacrylamide Based Cryogels – Synthesis and Biomimetic Modification for Stem Cell Applications
JF  - Physiological Research
VL  - 64
IS  - Suppl. 1
SP  - "S19"-"S27"
EP  - "S19"-"S27"
PB  - Fyziologický ústav AV ČR
SN  - 08628408
KW  - Hydrogels
KW  - Cryogels
KW  - Stem cells
KW  - Scaffolds
KW  - Mechanical properties
N2  - The design of favorable mechanical properties and suitable surface modifications of hydrogels in order to stimulate specific cell response is a great challenge. N-(2-Hydroxypropyl) methacryl-amide (HPMA) was utilized to form macroporous cryogel scaffolds for stem cell applications. Furthermore, one group of scaffolds was enhanced by copolymerization of HPMA with methacryloyl-GGGRGDS-OH peptide in an effort to integrate biomimetic adhesion sites. The cryogels were characterized by stiffness and equilibrium swelling measurements as well as by scanning electron microscopy. Cell culture experiments were performed with human adipose-derived stem cells and substrates were found completely non-toxic. Moreover, RGDS-enriched cryogels supported cell attachment, spreading and proliferation, so they can be considered suitable for designed aims.
ER  -

GOLUNOVA, A.; Josef JAROŠ; Veronika JURTÍKOVÁ; I. KOTELNIKOV; J. KOTEK; H. HLÍDKOVÁ; Libor STREIT; Aleš HAMPL; F. RYPÁČEK a V. PROKS. N-(2-Hydroxypropyl) Methacrylamide Based Cryogels – Synthesis and Biomimetic Modification for Stem Cell Applications. \textit{Physiological Research}. Praha: Fyziologický ústav AV ČR, 2015, roč.~64, Suppl. 1, s.~''S19''-''S27'', 9 s. ISSN~0862-8408.

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