The E3 ubiquitin ligase Mib1 regulates Plk4 and centriole
biogenesis

J 2015

The E3 ubiquitin ligase Mib1 regulates Plk4 and centriole biogenesis

ČAJÁNEK, Lukáš, Timo GLATTER a Erich A. NIGG

Základní údaje

Originální název

The E3 ubiquitin ligase Mib1 regulates Plk4 and centriole biogenesis

Autoři

ČAJÁNEK, Lukáš (203 Česká republika, garant, domácí), Timo GLATTER (756 Švýcarsko) a Erich A. NIGG (756 Švýcarsko)

Vydání

Journal of Cell Science, Cambridge, The Company of Biology Ltd. 2015, 0021-9533

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 4.706

Kód RIV

RIV/00216224:14110/15:00084871

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1242/jcs.166496

UT WoS

000354873100004

Klíčová slova anglicky

Cell cycle; Centriole; Centrosome; Kinase; Ubiquitin; Mib4; Plk4

Štítky

EL OK

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 25. 11. 2015 13:39, Soňa Böhmová

Anotace

V originále

Centrioles function as core components of centrosomes and as basal bodies for the formation of cilia and flagella. Thus, effective control of centriole numbers is essential for embryogenesis, tissue homeostasis and genome stability. In mammalian cells, the centriole duplication cycle is governed by Polo-like kinase 4 (Plk4). Here, we identify the E3 ubiquitin ligase Mind bomb (Mib1) as a new interaction partner of Plk4. We show that Mib1 localizes to centriolar satellites but redistributes to centrioles in response to conditions that induce centriole amplification. The E3 ligase activity of Mib1 triggers ubiquitylation of Plk4 on multiple sites, causing the formation of Lys11-, Lys29- and Lys48-ubiquitin linkages. These modifications control the abundance of Plk4 and its ability to interact with centrosomal proteins, thus counteracting centriole amplification induced by excess Plk4. Collectively, these results identify the interaction between Mib1 and Plk4 as a new and important element in the control of centriole homeostasis.

Návaznosti

MUNI/11/InGA15/2014, interní kód MU

Název: Analýza molekulárních a funkčních aspektů centriolárního cyklu v lidských buňkách

Investor: Masarykova univerzita, Analýza molekulárních a funkčních aspektů centriolárního cyklu v lidských buňkách

Citovat

ČAJÁNEK, Lukáš, Timo GLATTER a Erich A. NIGG. The E3 ubiquitin ligase Mib1 regulates Plk4 and centriole biogenesis. Journal of Cell Science. Cambridge: The Company of Biology Ltd., 2015, roč. 128, č. 9, s. 1674-1682. ISSN 0021-9533. Dostupné z: https://dx.doi.org/10.1242/jcs.166496.

@article{1318617,
   author = {Čajánek, Lukáš and Glatter, Timo and Nigg, Erich A.},
   article_location = {Cambridge},
   article_number = {9},
   doi = {http://dx.doi.org/10.1242/jcs.166496},
   keywords = {Cell cycle; Centriole; Centrosome; Kinase; Ubiquitin; Mib4; Plk4},
   language = {eng},
   issn = {0021-9533},
   journal = {Journal of Cell Science},
   title = {The E3 ubiquitin ligase Mib1 regulates Plk4 and centriole biogenesis},
   volume = {128},
   year = {2015}
}

TY  - JOUR
ID  - 1318617
AU  - Čajánek, Lukáš - Glatter, Timo - Nigg, Erich A.
PY  - 2015
TI  - The E3 ubiquitin ligase Mib1 regulates Plk4 and centriole biogenesis
JF  - Journal of Cell Science
VL  - 128
IS  - 9
SP  - 1674-1682
EP  - 1674-1682
PB  - The Company of Biology Ltd.
SN  - 00219533
KW  - Cell cycle
KW  - Centriole
KW  - Centrosome
KW  - Kinase
KW  - Ubiquitin
KW  - Mib4
KW  - Plk4
N2  - Centrioles function as core components of centrosomes and as basal bodies for the formation of cilia and flagella. Thus, effective control of centriole numbers is essential for embryogenesis, tissue homeostasis and genome stability. In mammalian cells, the centriole duplication cycle is governed by Polo-like kinase 4 (Plk4). Here, we identify the E3 ubiquitin ligase Mind bomb (Mib1) as a new interaction partner of Plk4. We show that Mib1 localizes to centriolar satellites but redistributes to centrioles in response to conditions that induce centriole amplification. The E3 ligase activity of Mib1 triggers ubiquitylation of Plk4 on multiple sites, causing the formation of Lys11-, Lys29- and Lys48-ubiquitin linkages. These modifications control the abundance of Plk4 and its ability to interact with centrosomal proteins, thus counteracting centriole amplification induced by excess Plk4. Collectively, these results identify the interaction between Mib1 and Plk4 as a new and important element in the control of centriole homeostasis.
ER  -

ČAJÁNEK, Lukáš, Timo GLATTER a Erich A. NIGG. The E3 ubiquitin ligase Mib1 regulates Plk4 and centriole biogenesis. \textit{Journal of Cell Science}. Cambridge: The Company of Biology Ltd., 2015, roč.~128, č.~9, s.~1674-1682. ISSN~0021-9533. Dostupné z: https://dx.doi.org/10.1242/jcs.166496.

Podrobný výpis o publikaci