Three-dimensional Imaging Reveals New Compartments and
Structural Adaptations in Odontoblasts

J 2015

Three-dimensional Imaging Reveals New Compartments and Structural Adaptations in Odontoblasts

SHAHIDI, Khatibi M.; Jan KŘIVÁNEK; N. KAUKUA; P. ERNFORS; L. HLADIK et. al.

Basic information

Original name

Three-dimensional Imaging Reveals New Compartments and Structural Adaptations in Odontoblasts

Authors

SHAHIDI, Khatibi M. (752 Sweden); Jan KŘIVÁNEK (203 Czech Republic, belonging to the institution); N. KAUKUA (752 Sweden); P. ERNFORS (752 Sweden); L. HLADIK (203 Czech Republic); V. KOSTAL (203 Czech Republic); S. MASICH (752 Sweden); Aleš HAMPL (203 Czech Republic, guarantor, belonging to the institution); V. CHUBANOV (276 Germany); T. GUDERMANN (276 Germany); R.A. ROMANOV (752 Sweden); T. HARKANY (752 Sweden); I. ADAMEYKO (40 Austria) and K. FRIED (752 Sweden)

Edition

Journal of Dental Research, Thousand Oaks, Sage Publication, 2015, 0022-0345

Other information

Language

English

Type of outcome

Article in a journal

Field of Study

Genetics and molecular biology

Country of publisher

United States of America

Confidentiality degree

is not subject to a state or trade secret

Impact factor

Impact factor: 4.602

RIV identification code

RIV/00216224:14110/15:00084873

Organization unit

Faculty of Medicine

DOI

https://doi.org/10.1177/0022034515580796

UT WoS

000356621000012

EID Scopus

2-s2.0-84935082368

Keywords in English

pulp biology; dentinogenesis; growth/development; protein expression; transgenic animals; cell morphology

Abstract

In the original language

In organized tissues, the precise geometry and the overall shape are critical for the specialized functions that the cells carry out. Odontoblasts are major matrix-producing cells of the tooth and have also been suggested to participate in sensory transmission. However, refined morphologic data on these important cells are limited, which hampers the analysis and understanding of their cellular functions. We took advantage of fluorescent color-coding genetic tracing to visualize and reconstruct in 3 dimensions single odontoblasts, pulp cells, and their assemblages. Our results show distinct structural features and compartments of odontoblasts at different stages of maturation, with regard to overall cellular shape, formation of the main process, orientation, and matrix deposition. We demonstrate previously unanticipated contacts between the processes of pulp cells and odontoblasts. All reported data are related to mouse incisor tooth. We also show that odontoblasts express TRPM5 and Piezo2 ion channels. Piezo2 is expressed ubiquitously, while TRPM5 is asymmetrically distributed with distinct localization to regions proximal to and within odontoblast processes.

Links

EE2.3.20.0180, research and development project

Name: Spolupráce mezi Masarykovou univerzitou a Karolinska Institutet, Stockholm na poli biomedicíny

MUNI/A/1014/2013, interní kód MU

Name: Zdroje pro tkáňové inženýrství 4

Investor: Masaryk University, Category A

Cite

SHAHIDI, Khatibi M.; Jan KŘIVÁNEK; N. KAUKUA; P. ERNFORS; L. HLADIK; V. KOSTAL; S. MASICH; Aleš HAMPL; V. CHUBANOV; T. GUDERMANN; R.A. ROMANOV; T. HARKANY; I. ADAMEYKO and K. FRIED. Three-dimensional Imaging Reveals New Compartments and Structural Adaptations in Odontoblasts. Journal of Dental Research. Thousand Oaks: Sage Publication, 2015, vol. 94, No 7, p. 945-954. ISSN 0022-0345. Available from: https://doi.org/10.1177/0022034515580796.

@article{1318625,
   author = {Shahidi, Khatibi M. and Křivánek, Jan and Kaukua, N. and Ernfors, P. and Hladik, L. and Kostal, V. and Masich, S. and Hampl, Aleš and Chubanov, V. and Gudermann, T. and Romanov, R.A. and Harkany, T. and Adameyko, I. and Fried, K.},
   article_location = {Thousand Oaks},
   article_number = {7},
   doi = {https://doi.org/10.1177/0022034515580796},
   keywords = {pulp biology; dentinogenesis; growth/development; protein expression; transgenic animals; cell morphology},
   language = {eng},
   issn = {0022-0345},
   journal = {Journal of Dental Research},
   title = {Three-dimensional Imaging Reveals New Compartments and Structural Adaptations in Odontoblasts},
   volume = {94},
   year = {2015}
}

TY  - JOUR
ID  - 1318625
AU  - Shahidi, Khatibi M. - Křivánek, Jan - Kaukua, N. - Ernfors, P. - Hladik, L. - Kostal, V. - Masich, S. - Hampl, Aleš - Chubanov, V. - Gudermann, T. - Romanov, R.A. - Harkany, T. - Adameyko, I. - Fried, K.
PY  - 2015
TI  - Three-dimensional Imaging Reveals New Compartments and Structural Adaptations in Odontoblasts
JF  - Journal of Dental Research
VL  - 94
IS  - 7
SP  - 945-954
EP  - 945-954
PB  - Sage Publication
SN  - 00220345
KW  - pulp biology
KW  - dentinogenesis
KW  - growth/development
KW  - protein expression
KW  - transgenic animals
KW  - cell morphology
N2  - In organized tissues, the precise geometry and the overall shape are critical for the specialized functions that the cells carry out. Odontoblasts are major matrix-producing cells of the tooth and have also been suggested to participate in sensory transmission. However, refined morphologic data on these important cells are limited, which hampers the analysis and understanding of their cellular functions. We took advantage of fluorescent color-coding genetic tracing to visualize and reconstruct in 3 dimensions single odontoblasts, pulp cells, and their assemblages. Our results show distinct structural features and compartments of odontoblasts at different stages of maturation, with regard to overall cellular shape, formation of the main process, orientation, and matrix deposition. We demonstrate previously unanticipated contacts between the processes of pulp cells and odontoblasts. All reported data are related to mouse incisor tooth. We also show that odontoblasts express TRPM5 and Piezo2 ion channels. Piezo2 is expressed ubiquitously, while TRPM5 is asymmetrically distributed with distinct localization to regions proximal to and within odontoblast processes.
ER  -

SHAHIDI, Khatibi M.; Jan KŘIVÁNEK; N. KAUKUA; P. ERNFORS; L. HLADIK; V. KOSTAL; S. MASICH; Aleš HAMPL; V. CHUBANOV; T. GUDERMANN; R.A. ROMANOV; T. HARKANY; I. ADAMEYKO and K. FRIED. Three-dimensional Imaging Reveals New Compartments and Structural Adaptations in Odontoblasts. \textit{Journal of Dental Research}. Thousand Oaks: Sage Publication, 2015, vol.~94, No~7, p.~945-954. ISSN~0022-0345. Available from: https://doi.org/10.1177/0022034515580796.

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