EXPRESSION AND CRYSTALLIZATION OF MYCOBACTERIAL
GALACTOFURANOSYL TRANSFERASE GLFT1

a 2015

EXPRESSION AND CRYSTALLIZATION OF MYCOBACTERIAL GALACTOFURANOSYL TRANSFERASE GLFT1

KYSEĽ, Peter; Gabriel DEMO; Stanislav HUSZÁR; Katarina MIKUŠOVÁ; Michaela WIMMEROVÁ et. al.

Základní údaje

Originální název

EXPRESSION AND CRYSTALLIZATION OF MYCOBACTERIAL GALACTOFURANOSYL TRANSFERASE GLFT1

Autoři

KYSEĽ, Peter (703 Slovensko, domácí); Gabriel DEMO (703 Slovensko, domácí); Stanislav HUSZÁR (703 Slovensko); Katarina MIKUŠOVÁ (703 Slovensko) a Michaela WIMMEROVÁ (203 Česká republika)

Vydání

XVII. setkání biochemiků a molekulárních biologů, 2015

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Kód RIV

RIV/00216224:14310/15:00085288

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-210-8015-7

Klíčová slova anglicky

mycobacterium; glycosyltransferase; purification; detergent;crystallography

Štítky

AKR

Změněno: 28. 4. 2016 15:12, Ing. Andrea Mikešková

Anotace

V originále

A number of glycosyltransferases is involved in the mycobacterial cell wall biosynthesis. The membrane associated galactofuranosyltransferase GlfT1 adds the first two galactofuranosyl residues of the galactan component of the arabinogalactan. Yet the esentiality of the GlfT1 for the proliferation of persistent mycobacterial pathogens provided a good reason to compose and optimise a combination of different biochemical approaches for purification and crystallization of this enzyme. We developed a multistep purification procedure to counter the pitfalls of proteolysis, chaperone contamination, precipitation and polydispersity. Two different conditions were identified to faciliate the crystallization of the fusion protein. X-ray diffraction data to 2.1 A were obtained. Solving of GlfT1 3D structure will eventually deliver structural insights into the first steps of galactan biosynthesis.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

Citovat

KYSEĽ, Peter; Gabriel DEMO; Stanislav HUSZÁR; Katarina MIKUŠOVÁ a Michaela WIMMEROVÁ. EXPRESSION AND CRYSTALLIZATION OF MYCOBACTERIAL GALACTOFURANOSYL TRANSFERASE GLFT1. In XVII. setkání biochemiků a molekulárních biologů. 2015. ISBN 978-80-210-8015-7.

@proceedings{1321653,
   author = {Kyseľ, Peter and Demo, Gabriel and Huszár, Stanislav and Mikušová, Katarina and Wimmerová, Michaela},
   booktitle = {XVII. setkání biochemiků a molekulárních biologů},
   keywords = {mycobacterium; glycosyltransferase; purification; detergent;crystallography},
   language = {eng},
   isbn = {978-80-210-8015-7},
   title = {EXPRESSION AND CRYSTALLIZATION OF MYCOBACTERIAL GALACTOFURANOSYL TRANSFERASE GLFT1},
   url = {http://orion.chemi.muni.cz/setkani/Setkani17/index.htm},
   year = {2015}
}

TY  - CONF
ID  - 1321653
AU  - Kyseľ, Peter - Demo, Gabriel - Huszár, Stanislav - Mikušová, Katarina - Wimmerová, Michaela
PY  - 2015
TI  - EXPRESSION AND CRYSTALLIZATION OF MYCOBACTERIAL GALACTOFURANOSYL TRANSFERASE GLFT1
SN  - 9788021080157
KW  - mycobacterium
KW  - glycosyltransferase
KW  - purification
KW  - detergent;crystallography
UR  - http://orion.chemi.muni.cz/setkani/Setkani17/index.htm
L2  - http://orion.chemi.muni.cz/setkani/Setkani17/index.htm
N2  - A number of glycosyltransferases is involved in the mycobacterial cell wall biosynthesis. The membrane associated galactofuranosyltransferase GlfT1 adds the first two galactofuranosyl residues of the galactan component of the arabinogalactan. Yet the esentiality of the GlfT1 for the proliferation of persistent mycobacterial pathogens provided a good reason to compose and optimise a combination of different biochemical approaches for purification and crystallization of this enzyme. We developed a multistep purification procedure to counter the pitfalls of proteolysis, chaperone contamination, precipitation and polydispersity. Two different conditions were identified to faciliate the crystallization of the fusion protein. X-ray diffraction data to 2.1 A were obtained. Solving of GlfT1 3D structure will eventually deliver structural insights into the first steps of galactan biosynthesis.
ER  -

KYSEĽ, Peter; Gabriel DEMO; Stanislav HUSZÁR; Katarina MIKUŠOVÁ a Michaela WIMMEROVÁ. EXPRESSION AND CRYSTALLIZATION OF MYCOBACTERIAL GALACTOFURANOSYL TRANSFERASE GLFT1. In \textit{XVII. setkání biochemiků a molekulárních biologů}. 2015. ISBN~978-80-210-8015-7.

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