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@proceedings{1321653, author = {Kyseľ, Peter and Demo, Gabriel and Huszár, Stanislav and Mikušová, Katarina and Wimmerová, Michaela}, booktitle = {XVII. setkání biochemiků a molekulárních biologů}, keywords = {mycobacterium; glycosyltransferase; purification; detergent;crystallography}, language = {eng}, isbn = {978-80-210-8015-7}, title = {EXPRESSION AND CRYSTALLIZATION OF MYCOBACTERIAL GALACTOFURANOSYL TRANSFERASE GLFT1}, url = {http://orion.chemi.muni.cz/setkani/Setkani17/index.htm}, year = {2015} }
TY - CONF ID - 1321653 AU - Kyseľ, Peter - Demo, Gabriel - Huszár, Stanislav - Mikušová, Katarina - Wimmerová, Michaela PY - 2015 TI - EXPRESSION AND CRYSTALLIZATION OF MYCOBACTERIAL GALACTOFURANOSYL TRANSFERASE GLFT1 SN - 9788021080157 KW - mycobacterium KW - glycosyltransferase KW - purification KW - detergent;crystallography UR - http://orion.chemi.muni.cz/setkani/Setkani17/index.htm L2 - http://orion.chemi.muni.cz/setkani/Setkani17/index.htm N2 - A number of glycosyltransferases is involved in the mycobacterial cell wall biosynthesis. The membrane associated galactofuranosyltransferase GlfT1 adds the first two galactofuranosyl residues of the galactan component of the arabinogalactan. Yet the esentiality of the GlfT1 for the proliferation of persistent mycobacterial pathogens provided a good reason to compose and optimise a combination of different biochemical approaches for purification and crystallization of this enzyme. We developed a multistep purification procedure to counter the pitfalls of proteolysis, chaperone contamination, precipitation and polydispersity. Two different conditions were identified to faciliate the crystallization of the fusion protein. X-ray diffraction data to 2.1 A were obtained. Solving of GlfT1 3D structure will eventually deliver structural insights into the first steps of galactan biosynthesis. ER -
KYSEĽ, Peter, Gabriel DEMO, Stanislav HUSZÁR, Katarina MIKUŠOVÁ a Michaela WIMMEROVÁ. EXPRESSION AND CRYSTALLIZATION OF MYCOBACTERIAL GALACTOFURANOSYL TRANSFERASE GLFT1. In \textit{XVII. setkání biochemiků a molekulárních biologů}. 2015. ISBN~978-80-210-8015-7.
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