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@article{1321695,
author = {Poborilova, Zuzana and Ohlsson, Anna B. and Berglund, Torkel and Vildova, Alena and Provaznik, Ivo and Babula, Petr},
article_location = {Oxford},
article_number = {MAY 2015},
doi = {http://dx.doi.org/10.1016/j.envexpbot.2015.01.005},
keywords = {DNA methylation; Juglone; Naphthoquinone; Programmed cell death; Reactive oxygen species; Tobacco BY-2 cell suspension culture},
language = {eng},
issn = {0098-8472},
journal = {Environmental and Experimental Botany},
title = {DNA hypomethylation concomitant with the overproduction of ROS induced by naphthoquinone juglone on tobacco BY-2 suspension cells},
volume = {113},
year = {2015}
}
TY - JOUR
ID - 1321695
AU - Poborilova, Zuzana - Ohlsson, Anna B. - Berglund, Torkel - Vildova, Alena - Provaznik, Ivo - Babula, Petr
PY - 2015
TI - DNA hypomethylation concomitant with the overproduction of ROS induced by naphthoquinone juglone on tobacco BY-2 suspension cells
JF - Environmental and Experimental Botany
VL - 113
IS - MAY 2015
SP - 28-39
EP - 28-39
PB - PERGAMON-ELSEVIER SCIENCE LTD
SN - 00988472
KW - DNA methylation
KW - Juglone
KW - Naphthoquinone
KW - Programmed cell death
KW - Reactive oxygen species
KW - Tobacco BY-2 cell suspension culture
N2 - Juglone, 5-hydroxy-1,4-naphthoquinone, is a naturally occurring plant secondary metabolite with allelopathic and cytotoxic properties. The cytotoxic effect of juglone and changes at the level of DNA methylation were investigated on a plant cell model, tobacco BY-2 cell suspension culture. We found that juglone in a concentration-dependent manner inhibits cell growth and causes creation of reactive oxygen species (ROS) that were visible mainly in nucleus, nucleolus, cytoplasm, and plasma membrane. Analysis of the changes in DNA methylation level using Luminometric Methylation Assay showed that juglone at the concentrations of 60 and 80 mu m causes hypomethylation of DNA. Decrease in DNA methylation was confirmed by the more sensitive technique, confocal immunofluorescence microscopy, already at 30 mu m, and was accompanied by significant overproduction of hydrogen peroxide, hydroxyl radicals, and superoxide anion radicals. The highest percentage of the signs of programed cell death, especially apoptic-like bodies that indicate DNA fragmentation was observed at 30 mu m of juglone. On the basis of results we suggest that overproduction of ROS is the main mechanism that causes hypomethylation of DNA, which may contribute to the initiation of the programmed cell death.
ER -
POBORILOVA, Zuzana, Anna B. OHLSSON, Torkel BERGLUND, Alena VILDOVA, Ivo PROVAZNIK a Petr BABULA. DNA hypomethylation concomitant with the overproduction of ROS induced by naphthoquinone juglone on tobacco BY-2 suspension cells. \textit{Environmental and Experimental Botany}. Oxford: PERGAMON-ELSEVIER SCIENCE LTD, 2015, roč.~113, MAY 2015, s.~28-39. ISSN~0098-8472. Dostupné z: https://dx.doi.org/10.1016/j.envexpbot.2015.01.005.
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