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@proceedings{1321855,
author = {Celá, Andrea and Mádr, Aleš and Dědová, Tereza and Pelcová, Marta and Glatz, Zdeněk},
booktitle = {22nd International Symposium on Electro- and Liquid Phase-Separation Techniques (ITP2015).},
keywords = {capillary electrophoresis, fluorescence detection, derivatization, amino acids},
language = {eng},
title = {Analysis of amino acids based on capillary electrophoresis with LIF detection using on-capillary derivatization},
year = {2015}
}
TY - CONF
ID - 1321855
AU - Celá, Andrea - Mádr, Aleš - Dědová, Tereza - Pelcová, Marta - Glatz, Zdeněk
PY - 2015
TI - Analysis of amino acids based on capillary electrophoresis with LIF detection using on-capillary derivatization
KW - capillary electrophoresis, fluorescence detection, derivatization, amino acids
N2 - Combination of capillary electrophoresis with laser-induced fluorescence detection provides high separation efficiency and sensitivity of the detection. Amino acids, which are very important for living organisms, and their levels in biological fluids can reveal health state of organisms, exhibit very low or no native fluorescence. As a result time consuming and laborious derivatization procedure has to be performed. Inner space of narrow capillary used in capillary electrophoresis can be used both for separation and derivatization. Optimization and comparison of pre-capillary and on-capillary derivatization of amino acids are subjects of this contribution.
ER -
CELÁ, Andrea, Aleš MÁDR, Tereza DĚDOVÁ, Marta PELCOVÁ a Zdeněk GLATZ. Analysis of amino acids based on capillary electrophoresis with LIF detection using on-capillary derivatization. In \textit{22nd International Symposium on Electro- and Liquid Phase-Separation Techniques (ITP2015).}. 2015.
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