Enzyme-linked electrochemical detection of DNA fragments
amplified by PCR in the presence of a biotinylated
deoxynucleoside triphosphate using disposable pencil graphite
electrodes

J 2015

Enzyme-linked electrochemical detection of DNA fragments amplified by PCR in the presence of a biotinylated deoxynucleoside triphosphate using disposable pencil graphite electrodes

HÁRONÍKOVÁ, Lucia; Jan ŠPAČEK; Medard PLUCNARA; Petra HORÁKOVÁ; Hana PIVOŇKOVÁ et al.

Základní údaje

Originální název

Enzyme-linked electrochemical detection of DNA fragments amplified by PCR in the presence of a biotinylated deoxynucleoside triphosphate using disposable pencil graphite electrodes

Autoři

HÁRONÍKOVÁ, Lucia; Jan ŠPAČEK; Medard PLUCNARA; Petra HORÁKOVÁ; Hana PIVOŇKOVÁ; Luděk HAVRAN; Arzum ERDEM a Miroslav FOJTA

Vydání

Monatshefte fur Chemie, Wien, Springer-Verlag, 2015, 0026-9247

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 1.131

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14740/15:00085534

Organizační jednotka

Středoevropský technologický institut

Klíčová slova anglicky

Enzymes; Nucleic acids; Voltammetry

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 5. 4. 2016 12:22, Mgr. Eva Špillingová

Anotace

V originále

In this report, we present a simple electrochemical detection protocol for the detection of specific PCR-amplified DNA fragments, based on incorporation of biotin tags into DNA amplicons during PCR run in the presence of a biotinylated nucleoside triphosphate. For detection, an enzyme-linked electrochemical system involving streptavidin-alkaline phosphatase conjugate attached to the biotinylated DNA, adsorbed at the surface of a disposable pencil graphite electrode, is used. The enzyme converts an inactive indicator, 1-naphthyl phosphate, into electrochemically oxidizable indicator 1-naphthol that is subsequently detected. Excellent selectivity of this fast, facile, and inexpensive analysis not requiring any sophisticated electrode modification and its applicability for off-line monitoring of DNA amplification is demonstrated. Applications of the technique include detection of the presence of specific nucleotide sequences in biological samples, such as sequences related to pathogenic microorganism or transgenes. [GRAPHICS] .

Citovat

HÁRONÍKOVÁ, Lucia; Jan ŠPAČEK; Medard PLUCNARA; Petra HORÁKOVÁ; Hana PIVOŇKOVÁ; Luděk HAVRAN; Arzum ERDEM a Miroslav FOJTA. Enzyme-linked electrochemical detection of DNA fragments amplified by PCR in the presence of a biotinylated deoxynucleoside triphosphate using disposable pencil graphite electrodes. Monatshefte fur Chemie. Wien: Springer-Verlag, 2015, roč. 146, č. 5, s. 849-855. ISSN 0026-9247. Dostupné z: https://doi.org/10.1007/s00706-015-1436-5.

@article{1322765,
   author = {Hároníková, Lucia and Špaček, Jan and Plucnara, Medard and Horáková, Petra and Pivoňková, Hana and Havran, Luděk and Erdem, Arzum and Fojta, Miroslav},
   article_location = {Wien},
   article_number = {5},
   doi = {https://doi.org/10.1007/s00706-015-1436-5},
   keywords = {Enzymes; Nucleic acids; Voltammetry},
   language = {eng},
   issn = {0026-9247},
   journal = {Monatshefte fur Chemie},
   title = {Enzyme-linked electrochemical detection of DNA fragments amplified by PCR in the presence of a biotinylated deoxynucleoside triphosphate using disposable pencil graphite electrodes},
   url = {http://download.springer.com/static/pdf/109/art%253A10.1007%252Fs00706-015-1436-5.pdf?originUrl=http%3A%2F%2Flink.springer.com%2Farticle%2F10.1007%2Fs00706-015-1436-5&token2=exp=1451982708~acl=%2Fstatic%2Fpdf%2F109%2Fart%25253A10.1007%25252Fs00706-015-14},
   volume = {146},
   year = {2015}
}

TY  - JOUR
ID  - 1322765
AU  - Hároníková, Lucia - Špaček, Jan - Plucnara, Medard - Horáková, Petra - Pivoňková, Hana - Havran, Luděk - Erdem, Arzum - Fojta, Miroslav
PY  - 2015
TI  - Enzyme-linked electrochemical detection of DNA fragments amplified by PCR in the presence of a biotinylated deoxynucleoside triphosphate using disposable pencil graphite electrodes
JF  - Monatshefte fur Chemie
VL  - 146
IS  - 5
SP  - 849-855
EP  - 849-855
PB  - Springer-Verlag
SN  - 00269247
KW  - Enzymes
KW  - Nucleic acids
KW  - Voltammetry
UR  - http://download.springer.com/static/pdf/109/art%253A10.1007%252Fs00706-015-1436-5.pdf?originUrl=http%3A%2F%2Flink.springer.com%2Farticle%2F10.1007%2Fs00706-015-1436-5&token2=exp=1451982708~acl=%2Fstatic%2Fpdf%2F109%2Fart%25253A10.1007%25252Fs00706-015-14
L2  - http://download.springer.com/static/pdf/109/art%253A10.1007%252Fs00706-015-1436-5.pdf?originUrl=http%3A%2F%2Flink.springer.com%2Farticle%2F10.1007%2Fs00706-015-1436-5&token2=exp=1451982708~acl=%2Fstatic%2Fpdf%2F109%2Fart%25253A10.1007%25252Fs00706-015-14
N2  - In this report, we present a simple electrochemical detection protocol for the detection of specific PCR-amplified DNA fragments, based on incorporation of biotin tags into DNA amplicons during PCR run in the presence of a biotinylated nucleoside triphosphate. For detection, an enzyme-linked electrochemical system involving streptavidin-alkaline phosphatase conjugate attached to the biotinylated DNA, adsorbed at the surface of a disposable pencil graphite electrode, is used. The enzyme converts an inactive indicator, 1-naphthyl phosphate, into electrochemically oxidizable indicator 1-naphthol that is subsequently detected. Excellent selectivity of this fast, facile, and inexpensive analysis not requiring any sophisticated electrode modification and its applicability for off-line monitoring of DNA amplification is demonstrated. Applications of the technique include detection of the presence of specific nucleotide sequences in biological samples, such as sequences related to pathogenic microorganism or transgenes. [GRAPHICS] .
ER  -

HÁRONÍKOVÁ, Lucia; Jan ŠPAČEK; Medard PLUCNARA; Petra HORÁKOVÁ; Hana PIVOŇKOVÁ; Luděk HAVRAN; Arzum ERDEM a Miroslav FOJTA. Enzyme-linked electrochemical detection of DNA fragments amplified by PCR in the presence of a biotinylated deoxynucleoside triphosphate using disposable pencil graphite electrodes. \textit{Monatshefte fur Chemie}. Wien: Springer-Verlag, 2015, roč.~146, č.~5, s.~849-855. ISSN~0026-9247. Dostupné z: https://doi.org/10.1007/s00706-015-1436-5.

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