2015
The effect of cryptogein with changed abilities to transfer sterols and altered charge distribution on extracellular alkalinization, ROS and NO generation, lipid peroxidation and LOX gene transcription in Nicotiana tabacum
PTÁČKOVÁ, Nikola, Jitka KLEMPOVÁ, Michal OBOŘIL, Sylvie NEDĚLOVÁ, Jan LOCHMAN et. al.Základní údaje
Originální název
The effect of cryptogein with changed abilities to transfer sterols and altered charge distribution on extracellular alkalinization, ROS and NO generation, lipid peroxidation and LOX gene transcription in Nicotiana tabacum
Autoři
PTÁČKOVÁ, Nikola (203 Česká republika, domácí), Jitka KLEMPOVÁ (203 Česká republika, domácí), Michal OBOŘIL (203 Česká republika, domácí), Sylvie NEDĚLOVÁ (203 Česká republika, domácí), Jan LOCHMAN (203 Česká republika, domácí) a Tomáš KAŠPAROVSKÝ (203 Česká republika, domácí)
Vydání
Plant Physiology and Biochemistry, Paris, Elsevier, 2015, 0981-9428
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
10600 1.6 Biological sciences
Stát vydavatele
Francie
Utajení
není předmětem státního či obchodního tajemství
Impakt faktor
Impact factor: 2.928
Kód RIV
RIV/00216224:14310/15:00081485
Organizační jednotka
Přírodovědecká fakulta
UT WoS
000366950100010
Klíčová slova anglicky
Cryptogein; Hydroxy fatty acids analysis; Hypersensitive response; Lipid peroxidation; Mutant proteins; Nicotiana tabaccum
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 12. 4. 2016 15:19, Ing. Andrea Mikešková
Anotace
V originále
Cryptogein, a protein from oomycete Phytophthora cryptogea, induces a hypersensitive cell death in Nicotiana tabacum. We prepared a new series of cryptogein mutant proteins with altered abilities to bind sterols and with altered charge distribution in the proteins. The effect of the mutations on the cryptogein ability to induce plant defence mechanisms associated with hypersensitive cell death were examined. Our results with new mutants support the previous findings that the sterol binding does not influence synthesis of ROS, cytosol acidification and development of leaf necrosis as these events seem to be more likely affected by the charge distribution and the overall protein structure. This hypothesis was also applicable on other mechanisms involved in the execution of plant cell death such as the NO generation, the stimulation of lipid peroxidation (determination of malondialdehyde and hydroxy fatty acids levels) and LOX gene transcription. In addition, the ability to bind sterols was found to serve not only for pathogen utilisation in its own metabolism but also to have an important function for the destabilization of plant membrane facilitating the pathogen spread inside the plant tissue as well as intensively contributing to the development of plant cell death. Considering the insertion of charged amino acid residues in the protein structure, the change localized in the protein surface affected its biological activity more effectively than that change inside the protein cavity. Moreover, the insertion of negative charged amino acids influenced mainly the events involved in the early phase of defence reaction, while the positive residues affected especially the necrotic activity of cryptogein.
Návaznosti
GAP501/11/1003, projekt VaV |
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