MiR-205 functions as a tumor suppressor in adenocarcinoma and
an oncogene in squamous cell carcinoma of esophagus

J 2016

MiR-205 functions as a tumor suppressor in adenocarcinoma and an oncogene in squamous cell carcinoma of esophagus

HÉŽOVÁ, Renata; Alena KOVAŘÍKOVÁ; Josef SROVNAL; Milada ZEMANOVÁ; Tomáš HARUSTIAK et. al.

Základní údaje

Originální název

MiR-205 functions as a tumor suppressor in adenocarcinoma and an oncogene in squamous cell carcinoma of esophagus

Autoři

HÉŽOVÁ, Renata (203 Česká republika, domácí); Alena KOVAŘÍKOVÁ (203 Česká republika, domácí); Josef SROVNAL (203 Česká republika); Milada ZEMANOVÁ (203 Česká republika); Tomáš HARUSTIAK (703 Slovensko); Jiří EHRMANN (203 Česká republika); Marian HAJDÚCH (203 Česká republika); Milana ŠACHLOVÁ (203 Česká republika); Marek SVOBODA (203 Česká republika) a Ondřej SLABÝ (203 Česká republika, garant, domácí)

Vydání

Tumor Biology, Dordrecht, Springer, 2016, 1010-4283

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30200 3.2 Clinical medicine

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.650

Kód RIV

RIV/00216224:14740/16:00088840

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1007/s13277-015-4656-8

UT WoS

000376464700100

EID Scopus

2-s2.0-84951915614

Klíčová slova anglicky

Esophageal adenocarcinoma; Esophageal squamous cell carcinoma; miR-205; Tumor suppressor; Oncogene

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 27. 4. 2017 13:03, Mgr. Eva Špillingová

Anotace

V originále

Esophageal cancer is a malignant disease with poor prognosis, increasing incidence, and ineffective treatment options. MicroRNAs are post-transcriptional regulators of gene expression involved in many biological processes including carcinogenesis. We determined miR-205 expression levels in tumor/non-tumor tissues of 45 esophageal cancer patients using qPCR and found that decreased level of miR-205 in tumor tissue correlates with poor overall survival in esophageal adenocarcinoma patients. Further, we observed significantly higher levels of miR-205 in tumor tissue of esophageal squamous cell carcinoma. Ectopic overexpression of miR-205 in adenocarcinoma cell line SK-GT-4 led to decreased cell proliferation, cell cycle arrest in G1, and decreased migration ability. Conversely, in squamous cell line KYSE-150, same effects like inhibition of proliferation, migration, and colony-forming potential and cell cycle arrest in G2 were observed after silencing of miR-205. We performed global gene expression profiling and revealed that suppressive functioning of miR-205 in adenocarcinoma could be realized through regulation of epithelial-mesenchymal transition (EMT), whereas oncogenic in squamous cell carcinoma by regulation of metalloproteinase 10. Our results suggest that miR-205 could serve as biomarker in esophageal cancer and acts as a tumor suppressor in esophageal adenocarcinoma and oncogene in esophageal squamous cell carcinoma.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

NT13547, projekt VaV

Název: Studium mikroRNA a genů asociovaných s procesem epiteliálně-mezenchymální tranzice jako potenciálních markerů pro predikci rizika a časný záchyt metastazování u pacientů s renálním karcinomem

NV15-34678A, projekt VaV

Název: Molekulární prognostické a prediktivní faktory u pacientů s metastatickým renálním karcinomem léčených tyrozinkinázovými inhibitory

Citovat

HÉŽOVÁ, Renata; Alena KOVAŘÍKOVÁ; Josef SROVNAL; Milada ZEMANOVÁ; Tomáš HARUSTIAK; Jiří EHRMANN; Marian HAJDÚCH; Milana ŠACHLOVÁ; Marek SVOBODA a Ondřej SLABÝ. MiR-205 functions as a tumor suppressor in adenocarcinoma and an oncogene in squamous cell carcinoma of esophagus. Tumor Biology. Dordrecht: Springer, 2016, roč. 37, č. 6, s. 8007-8018. ISSN 1010-4283. Dostupné z: https://dx.doi.org/10.1007/s13277-015-4656-8.

@article{1331432,
   author = {Héžová, Renata and Kovaříková, Alena and Srovnal, Josef and Zemanová, Milada and Harustiak, Tomáš and Ehrmann, Jiří and Hajdúch, Marian and Šachlová, Milana and Svoboda, Marek and Slabý, Ondřej},
   article_location = {Dordrecht},
   article_number = {6},
   doi = {http://dx.doi.org/10.1007/s13277-015-4656-8},
   keywords = {Esophageal adenocarcinoma; Esophageal squamous cell carcinoma; miR-205; Tumor suppressor; Oncogene},
   language = {eng},
   issn = {1010-4283},
   journal = {Tumor Biology},
   title = {MiR-205 functions as a tumor suppressor in adenocarcinoma and an oncogene in squamous cell carcinoma of esophagus},
   url = {http://link.springer.com/article/10.1007%2Fs13277-015-4656-8},
   volume = {37},
   year = {2016}
}

TY  - JOUR
ID  - 1331432
AU  - Héžová, Renata - Kovaříková, Alena - Srovnal, Josef - Zemanová, Milada - Harustiak, Tomáš - Ehrmann, Jiří - Hajdúch, Marian - Šachlová, Milana - Svoboda, Marek - Slabý, Ondřej
PY  - 2016
TI  - MiR-205 functions as a tumor suppressor in adenocarcinoma and an oncogene in squamous cell carcinoma of esophagus
JF  - Tumor Biology
VL  - 37
IS  - 6
SP  - 8007-8018
EP  - 8007-8018
PB  - Springer
SN  - 10104283
KW  - Esophageal adenocarcinoma
KW  - Esophageal squamous cell carcinoma
KW  - miR-205
KW  - Tumor suppressor
KW  - Oncogene
UR  - http://link.springer.com/article/10.1007%2Fs13277-015-4656-8
L2  - http://link.springer.com/article/10.1007%2Fs13277-015-4656-8
N2  - Esophageal cancer is a malignant disease with poor prognosis, increasing incidence, and ineffective treatment options. MicroRNAs are post-transcriptional regulators of gene expression involved in many biological processes including carcinogenesis. We determined miR-205 expression levels in tumor/non-tumor tissues of 45 esophageal cancer patients using qPCR and found that decreased level of miR-205 in tumor tissue correlates with poor overall survival in esophageal adenocarcinoma patients. Further, we observed significantly higher levels of miR-205 in tumor tissue of esophageal squamous cell carcinoma. Ectopic overexpression of miR-205 in adenocarcinoma cell line SK-GT-4 led to decreased cell proliferation, cell cycle arrest in G1, and decreased migration ability. Conversely, in squamous cell line KYSE-150, same effects like inhibition of proliferation, migration, and colony-forming potential and cell cycle arrest in G2 were observed after silencing of miR-205. We performed global gene expression profiling and revealed that suppressive functioning of miR-205 in adenocarcinoma could be realized through regulation of epithelial-mesenchymal transition (EMT), whereas oncogenic in squamous cell carcinoma by regulation of metalloproteinase 10. Our results suggest that miR-205 could serve as biomarker in esophageal cancer and acts as a tumor suppressor in esophageal adenocarcinoma and oncogene in esophageal squamous cell carcinoma.
ER  -

HÉŽOVÁ, Renata; Alena KOVAŘÍKOVÁ; Josef SROVNAL; Milada ZEMANOVÁ; Tomáš HARUSTIAK; Jiří EHRMANN; Marian HAJDÚCH; Milana ŠACHLOVÁ; Marek SVOBODA a Ondřej SLABÝ. MiR-205 functions as a tumor suppressor in adenocarcinoma and an oncogene in squamous cell carcinoma of esophagus. \textit{Tumor Biology}. Dordrecht: Springer, 2016, roč.~37, č.~6, s.~8007-8018. ISSN~1010-4283. Dostupné z: https://dx.doi.org/10.1007/s13277-015-4656-8.

Přehled o publikaci