Interaction of RECQ4 and MCM10 is important for efficient DNA
replication origin firing in human cells

J 2015

Interaction of RECQ4 and MCM10 is important for efficient DNA replication origin firing in human cells

KLISZCZAK, Maciej, Hana SEDLÁČKOVÁ, Ganesha P. PITCHAI, Werner W. STREICHER, Lumír KREJČÍ et. al.

Základní údaje

Originální název

Interaction of RECQ4 and MCM10 is important for efficient DNA replication origin firing in human cells

Autoři

KLISZCZAK, Maciej (208 Dánsko), Hana SEDLÁČKOVÁ (203 Česká republika, domácí), Ganesha P. PITCHAI (208 Dánsko), Werner W. STREICHER (208 Dánsko), Lumír KREJČÍ (203 Česká republika, garant, domácí) a Ian D. HICKSON (208 Dánsko)

Vydání

Oncotarget, New York, Impact Journals LLC, 2015, 1949-2553

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 5.008

Kód RIV

RIV/00216224:14110/15:00081496

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.18632/oncotarget.6342

UT WoS

000366115500008

Klíčová slova anglicky

DNA replication; RecQ helicases; minichromosome maintenance proteins; Chromosome Section

Štítky

EL OK, podil

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 20. 1. 2016 14:49, Ing. Mgr. Věra Pospíšilíková

Anotace

V originále

DNA replication is a highly coordinated process that is initiated at multiple replication origins in eukaryotes. These origins are bound by the origin recognition complex (ORC), which subsequently recruits the Mcm2-7 replicative helicase in a Cdt1/Cdc6-dependent manner. In budding yeast, two essential replication factors, Sld2 and Mcm10, are then important for the activation of replication origins. In humans, the putative Sld2 homolog, RECQ4, interacts with MCM10. Here, we have identified two mutants of human RECQ4 that are deficient in binding to MCM10. We show that these RECQ4 variants are able to complement the lethality of an avian cell RECQ4 deletion mutant, indicating that the essential function of RECQ4 in vertebrates is unlikely to require binding to MCM10. Nevertheless, we show that the RECQ4-MCM10 interaction is important for efficient replication origin firing.

Návaznosti

GAP207/12/2323, projekt VaV

Název: Endonuleazová a translokázová aktivita v restričních-modifikáčních komplexéch typu I

Investor: Grantová agentura ČR, Endonuleázová a translokázová aktivita v restrikčních-modifikačních komplexech typu I

GA13-26629S, projekt VaV

Název: SUMO a stability genomu

Investor: Grantová agentura ČR, SUMO a stability genomu

MUNI/M/1894/2014, interní kód MU

Název: Development of new MUS81 nuclease inhibitors as chemical biology probe with clinical progression

Investor: Masarykova univerzita, Development of new MUS81 nuclease inhibitors as chemical biology probe with clinical progression, INTERDISCIPLINARY - Mezioborové výzkumné projekty

Citovat

KLISZCZAK, Maciej, Hana SEDLÁČKOVÁ, Ganesha P. PITCHAI, Werner W. STREICHER, Lumír KREJČÍ a Ian D. HICKSON. Interaction of RECQ4 and MCM10 is important for efficient DNA replication origin firing in human cells. Oncotarget. New York: Impact Journals LLC, 2015, roč. 6, č. 38, s. 40464-40479. ISSN 1949-2553. Dostupné z: https://dx.doi.org/10.18632/oncotarget.6342.

@article{1331446,
   author = {Kliszczak, Maciej and Sedláčková, Hana and Pitchai, Ganesha P. and Streicher, Werner W. and Krejčí, Lumír and Hickson, Ian D.},
   article_location = {New York},
   article_number = {38},
   doi = {http://dx.doi.org/10.18632/oncotarget.6342},
   keywords = {DNA replication; RecQ helicases; minichromosome maintenance proteins; Chromosome Section},
   language = {eng},
   issn = {1949-2553},
   journal = {Oncotarget},
   title = {Interaction of RECQ4 and MCM10 is important for efficient DNA replication origin firing in human cells},
   volume = {6},
   year = {2015}
}

TY  - JOUR
ID  - 1331446
AU  - Kliszczak, Maciej - Sedláčková, Hana - Pitchai, Ganesha P. - Streicher, Werner W. - Krejčí, Lumír - Hickson, Ian D.
PY  - 2015
TI  - Interaction of RECQ4 and MCM10 is important for efficient DNA replication origin firing in human cells
JF  - Oncotarget
VL  - 6
IS  - 38
SP  - 40464-40479
EP  - 40464-40479
PB  - Impact Journals LLC
SN  - 19492553
KW  - DNA replication
KW  - RecQ helicases
KW  - minichromosome maintenance proteins
KW  - Chromosome Section
N2  - DNA replication is a highly coordinated process that is initiated at multiple replication origins in eukaryotes. These origins are bound by the origin recognition complex (ORC), which subsequently recruits the Mcm2-7 replicative helicase in a Cdt1/Cdc6-dependent manner. In budding yeast, two essential replication factors, Sld2 and Mcm10, are then important for the activation of replication origins. In humans, the putative Sld2 homolog, RECQ4, interacts with MCM10. Here, we have identified two mutants of human RECQ4 that are deficient in binding to MCM10. We show that these RECQ4 variants are able to complement the lethality of an avian cell RECQ4 deletion mutant, indicating that the essential function of RECQ4 in vertebrates is unlikely to require binding to MCM10. Nevertheless, we show that the RECQ4-MCM10 interaction is important for efficient replication origin firing.
ER  -

KLISZCZAK, Maciej, Hana SEDLÁČKOVÁ, Ganesha P. PITCHAI, Werner W. STREICHER, Lumír KREJČÍ a Ian D. HICKSON. Interaction of RECQ4 and MCM10 is important for efficient DNA replication origin firing in human cells. \textit{Oncotarget}. New York: Impact Journals LLC, 2015, roč.~6, č.~38, s.~40464-40479. ISSN~1949-2553. Dostupné z: https://dx.doi.org/10.18632/oncotarget.6342.

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