The Effect of Voltage-Sensitive Dye di-4-ANEPPS on Heart Rate
Variability in Langendorff-Perfused Isolated Rabbit Heart

D 2015

The Effect of Voltage-Sensitive Dye di-4-ANEPPS on Heart Rate Variability in Langendorff-Perfused Isolated Rabbit Heart

JANOUŠEK, Oto; Marina RONZHINA; Jakub HEJČ; Veronika OLEJNÍČKOVÁ; Tibor STRAČINA et al.

Základní údaje

Originální název

The Effect of Voltage-Sensitive Dye di-4-ANEPPS on Heart Rate Variability in Langendorff-Perfused Isolated Rabbit Heart

Autoři

JANOUŠEK, Oto; Marina RONZHINA; Jakub HEJČ; Veronika OLEJNÍČKOVÁ; Tibor STRAČINA ; Kateřina FIALOVÁ; Marie NOVÁKOVÁ; Ivo PROVAZNÍ a Jana KOLÁŘOVÁ

Vydání

Washington, Computing in Cardiology 2015, od s. 1049-1052, 4 s. 2015

Nakladatel

IEEE Computer Society

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

30105 Physiology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

tištěná verze "print"

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/15:00081552

Organizační jednotka

Lékařská fakulta

ISSN

Klíčová slova anglicky

di-4-ANEPPS;Voltage-Sensitive Dye

Štítky

EL OK

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 8. 2. 2016 14:14, Soňa Böhmová

Anotace

V originále

Optical mapping of heart electrical activity in Langendorff-perfused isolated hearts is based on voltagesensitive dyes, of which the most commonly used one is di-4-ANEPPS. Prolongation of action potential duration by di-4-ANEPPS was reported in isolated cells; however, this phenomenon was not observed in the whole heart model. In order to search for other adverse phenomena of di- 4-ANEPPS use, its effect on heart rate variability (HRV) was investigated during staining and washout periods in five New Zealand White rabbit isolated hearts. Time domain, frequency domain and non-linear HRV parameters (21 in total) revealed that there are no significant changes between control, staining and washout periods. Voltage-sensitive dye di-4-ANEPPS does not affect HRV when applied into the isolated heart coronary system. It can be concluded that di-4-ANEPPS may be safely used in studies combining optical mapping and HRV analysis

Návaznosti

GAP102/12/2034, projekt VaV

Název: Analýza vztahu mezi elektrickými ději a průtokem krve u srdečních komor

Investor: Grantová agentura ČR, Analýza vztahu mezi elektrickými ději a průtokem krve u srdečních komor

MUNI/A/1326/2014, interní kód MU

Název: Kardiovaskulární systém od buňky k lůžku pacienta (Akronym: KASBUNPAC)

Investor: Masarykova univerzita, Kardiovaskulární systém od buňky k lůžku pacienta, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

JANOUŠEK, Oto; Marina RONZHINA; Jakub HEJČ; Veronika OLEJNÍČKOVÁ; Tibor STRAČINA; Kateřina FIALOVÁ; Marie NOVÁKOVÁ; Ivo PROVAZNÍ a Jana KOLÁŘOVÁ. The Effect of Voltage-Sensitive Dye di-4-ANEPPS on Heart Rate Variability in Langendorff-Perfused Isolated Rabbit Heart. In Alan Murray. Computing in Cardiology 2015. Washington: IEEE Computer Society, 2015, s. 1049-1052. ISSN 2325-8861.

@inproceedings{1334716,
   author = {Janoušek, Oto and Ronzhina, Marina and Hejč, Jakub and Olejníčková, Veronika and Stračina, Tibor and Fialová, Kateřina and Nováková, Marie and Provazní, Ivo and Kolářová, Jana},
   address = {Washington},
   booktitle = {Computing in Cardiology 2015},
   editor = {Alan Murray},
   keywords = {di-4-ANEPPS;Voltage-Sensitive Dye},
   howpublished = {tištěná verze "print"},
   language = {eng},
   location = {Washington},
   pages = {1049-1052},
   publisher = {IEEE Computer Society},
   title = {The Effect of Voltage-Sensitive Dye di-4-ANEPPS on Heart Rate Variability in Langendorff-Perfused Isolated Rabbit Heart},
   year = {2015}
}

TY  - CONF
ID  - 1334716
AU  - Janoušek, Oto - Ronzhina, Marina - Hejč, Jakub - Olejníčková, Veronika - Stračina, Tibor - Fialová, Kateřina - Nováková, Marie - Provazní, Ivo - Kolářová, Jana
PY  - 2015
TI  - The Effect of Voltage-Sensitive Dye di-4-ANEPPS on Heart Rate Variability in Langendorff-Perfused Isolated Rabbit Heart
PB  - IEEE Computer Society
CY  - Washington
KW  - di-4-ANEPPS;Voltage-Sensitive Dye
N2  - Optical mapping of heart electrical activity in Langendorff-perfused isolated hearts is based on voltagesensitive dyes, of which the most commonly used one is di-4-ANEPPS. Prolongation of action potential duration by di-4-ANEPPS was reported in isolated cells; however, this phenomenon was not observed in the whole heart model. In order to search for other adverse phenomena of di- 4-ANEPPS use, its effect on heart rate variability (HRV) was investigated during staining and washout periods in five New Zealand White rabbit isolated hearts. Time domain, frequency domain and non-linear HRV parameters (21 in total) revealed that there are no significant changes between control, staining and washout periods. Voltage-sensitive dye di-4-ANEPPS does not affect HRV when applied into the isolated heart coronary system. It can be concluded that di-4-ANEPPS may be safely used in studies combining optical mapping and HRV analysis
ER  -

JANOUŠEK, Oto; Marina RONZHINA; Jakub HEJČ; Veronika OLEJNÍČKOVÁ; Tibor STRAČINA; Kateřina FIALOVÁ; Marie NOVÁKOVÁ; Ivo PROVAZNÍ a Jana KOLÁŘOVÁ. The Effect of Voltage-Sensitive Dye di-4-ANEPPS on Heart Rate Variability in Langendorff-Perfused Isolated Rabbit Heart. In Alan Murray. \textit{Computing in Cardiology 2015}. Washington: IEEE Computer Society, 2015, s.~1049-1052. ISSN~2325-8861.

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