Simple non-invasive analysis of embryonic stem cell-derived
cardiomyocytes beating in vitro

J 2016

Simple non-invasive analysis of embryonic stem cell-derived cardiomyocytes beating in vitro

RADASZKIEWICZ, Katarzyna Anna; Dominika SÝKOROVÁ; Pavel KARAS; Jana KUDOVÁ; Lukáš KOHÚT et al.

Základní údaje

Originální název

Simple non-invasive analysis of embryonic stem cell-derived cardiomyocytes beating in vitro

Autoři

RADASZKIEWICZ, Katarzyna Anna; Dominika SÝKOROVÁ; Pavel KARAS; Jana KUDOVÁ; Lukáš KOHÚT; Lucia BINÓ; Josef VEČEŘA; Jan VÍTEČEK; Lukáš KUBALA a Jiří PACHERNÍK

Vydání

Review of Scientific Instruments, Melville (USA), American Institute of Physics, 2016, 0034-6748

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30105 Physiology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 1.515

Kód RIV

RIV/00216224:14310/16:00089517

Organizační jednotka

Přírodovědecká fakulta

DOI

https://doi.org/10.1063/1.4941776

UT WoS

000371740900257

EID Scopus

2-s2.0-84959036496

Klíčová slova česky

kardiomyocyty; digitalni video; tepani

Klíčová slova anglicky

cardiomyocytes; digital video; beating

Štítky

AKR, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 13. 4. 2017 22:21, Ing. Andrea Mikešková

Anotace

V originále

The analysis of digital video output enables the non-invasive screening of various active biological processes. For the monitoring and computing of the beating parameters of cardiomyocytes in vitro, CB Analyser (cardiomyocyte beating analyser) software was developed. This software is based on image analysis of the video recording of beating cardiomyocytes. CB Analyser was tested using cardiomyocytes derived from mouse embryonic stem cells at different stages of cardiomyogenesis. We observed that during differentiation (from day 18), the beat peak width decreased, which corresponded to the increased speed of an individual pulse. However, the beating frequency did not change. Further, the effects of epinephrine modulating mature cardiomyocyte functions were tested to validate the CB Analyser analysis. In conclusion, data show that CB Analyser is a useful tool for evaluating the functions of both developing and mature cardiomyocytes under various conditions in vitro

Citovat

RADASZKIEWICZ, Katarzyna Anna; Dominika SÝKOROVÁ; Pavel KARAS; Jana KUDOVÁ; Lukáš KOHÚT; Lucia BINÓ; Josef VEČEŘA; Jan VÍTEČEK; Lukáš KUBALA a Jiří PACHERNÍK. Simple non-invasive analysis of embryonic stem cell-derived cardiomyocytes beating in vitro. Review of Scientific Instruments. Melville (USA): American Institute of Physics, 2016, roč. 87, č. 2, s. "nestrankovano", 7 s. ISSN 0034-6748. Dostupné z: https://doi.org/10.1063/1.4941776.

@article{1338725,
   author = {Radaszkiewicz, Katarzyna Anna and Sýkorová, Dominika and Karas, Pavel and Kudová, Jana and Kohút, Lukáš and Binó, Lucia and Večeřa, Josef and Víteček, Jan and Kubala, Lukáš and Pacherník, Jiří},
   article_location = {Melville (USA)},
   article_number = {2},
   doi = {https://doi.org/10.1063/1.4941776},
   keywords = {cardiomyocytes; digital video; beating},
   language = {eng},
   issn = {0034-6748},
   journal = {Review of Scientific Instruments},
   title = {Simple non-invasive analysis of embryonic stem cell-derived cardiomyocytes beating in vitro},
   url = {http://scitation.aip.org/docserver/fulltext/aip/journal/rsi/87/2/1.4941776.pdf?expires=1457558113&id=id&accname=guest&checksum=4E6E84A242B8B6216A78B92ACD4D6AE6},
   volume = {87},
   year = {2016}
}

TY  - JOUR
ID  - 1338725
AU  - Radaszkiewicz, Katarzyna Anna - Sýkorová, Dominika - Karas, Pavel - Kudová, Jana - Kohút, Lukáš - Binó, Lucia - Večeřa, Josef - Víteček, Jan - Kubala, Lukáš - Pacherník, Jiří
PY  - 2016
TI  - Simple non-invasive analysis of embryonic stem cell-derived cardiomyocytes beating in vitro
JF  - Review of Scientific Instruments
VL  - 87
IS  - 2
SP  - "nestrankovano"
EP  - "nestrankovano"
PB  - American Institute of Physics
SN  - 00346748
KW  - cardiomyocytes
KW  - digital video
KW  - beating
UR  - http://scitation.aip.org/docserver/fulltext/aip/journal/rsi/87/2/1.4941776.pdf?expires=1457558113&id=id&accname=guest&checksum=4E6E84A242B8B6216A78B92ACD4D6AE6
N2  - The analysis of digital video output enables the non-invasive screening of various active biological processes. For the monitoring and computing of the beating parameters of cardiomyocytes in vitro, CB Analyser (cardiomyocyte beating analyser) software was developed. This software is based on image analysis of the video recording of beating cardiomyocytes. CB Analyser was tested using cardiomyocytes derived from mouse embryonic stem cells at different stages of cardiomyogenesis. We observed that during differentiation (from day 18), the beat peak width decreased, which corresponded to the increased speed of an individual pulse. However, the beating frequency did not change. Further, the effects of epinephrine modulating mature cardiomyocyte functions were tested to validate the CB Analyser analysis. In conclusion, data show that CB Analyser is a useful tool for evaluating the functions of both developing and mature cardiomyocytes under various conditions in vitro
ER  -

RADASZKIEWICZ, Katarzyna Anna; Dominika SÝKOROVÁ; Pavel KARAS; Jana KUDOVÁ; Lukáš KOHÚT; Lucia BINÓ; Josef VEČEŘA; Jan VÍTEČEK; Lukáš KUBALA a Jiří PACHERNÍK. Simple non-invasive analysis of embryonic stem cell-derived cardiomyocytes beating in vitro. \textit{Review of Scientific Instruments}. Melville (USA): American Institute of Physics, 2016, roč.~87, č.~2, s.~''nestrankovano'', 7 s. ISSN~0034-6748. Dostupné z: https://doi.org/10.1063/1.4941776.

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