Conducting polyaniline based cell culture substrate for
embryonic stem cells and embryoid bodies

J 2015

Conducting polyaniline based cell culture substrate for embryonic stem cells and embryoid bodies

BOBER, Patrycja; Petr HUMPOLÍČEK; Jiří PACHERNÍK; Jaroslav STEJSKAL; Tom LINDFORS et al.

Základní údaje

Originální název

Conducting polyaniline based cell culture substrate for embryonic stem cells and embryoid bodies

Autoři

BOBER, Patrycja; Petr HUMPOLÍČEK; Jiří PACHERNÍK; Jaroslav STEJSKAL a Tom LINDFORS

Vydání

RSC Advances, Cambridge, Royal Society of Chemistry, 2015, 2046-2069

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10404 Polymer science

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.289

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/15:00087515

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova česky

Polyanilin; kmenove buňky

Klíčová slova anglicky

Polyaniline; stem cells

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 18. 6. 2020 11:12, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

In this work, thin films consisting of electrically conducting polyaniline (PANI) and poly(2-acrylamido-2-methyl-1-propanesulfonate) (PAMPSA) have been used as cell culture substrates for embryonic stem cells (ESC) and embryoid bodies (EMB). The PANI–PAMPSA films having fibrillar morphology were electrochemically polymerized in a single-step by cyclic voltammetry from an aqueous solution containing aniline and PAMPSA. UV-visible spectroscopy showed that the PANI films were electrically conducting still at pH 10. This makes them suitable for tissue engineering applications operating at physiological pH, in contrast to the commonly used PANI hydrochloride films which loose their electrical conductivity at pH 4 and higher. Our results reveal that the PANI–PAMPSA films allow only for limited ESC adhesion and growth. The inhibition of the EMB growth and adhesion on the PANI–PAMPSA surface in serum-free medium indicates that it can be used as a cell-selective substrate for the growth of only some specific differentiated EMB cell types.

Citovat

BOBER, Patrycja; Petr HUMPOLÍČEK; Jiří PACHERNÍK; Jaroslav STEJSKAL a Tom LINDFORS. Conducting polyaniline based cell culture substrate for embryonic stem cells and embryoid bodies. RSC Advances. Cambridge: Royal Society of Chemistry, 2015, roč. 5, č. 62, s. 50328-50335. ISSN 2046-2069. Dostupné z: https://doi.org/10.1039/C5RA07504A.

@article{1339418,
   author = {Bober, Patrycja and Humpolíček, Petr and Pacherník, Jiří and Stejskal, Jaroslav and Lindfors, Tom},
   article_location = {Cambridge},
   article_number = {62},
   doi = {https://doi.org/10.1039/C5RA07504A},
   keywords = {Polyaniline; stem cells},
   language = {eng},
   issn = {2046-2069},
   journal = {RSC Advances},
   title = {Conducting polyaniline based cell culture substrate for embryonic stem cells and embryoid bodies},
   url = {http://pubs.rsc.org/en/Content/ArticleLanding/2015/RA/C5RA07504A#!divAbstract},
   volume = {5},
   year = {2015}
}

TY  - JOUR
ID  - 1339418
AU  - Bober, Patrycja - Humpolíček, Petr - Pacherník, Jiří - Stejskal, Jaroslav - Lindfors, Tom
PY  - 2015
TI  - Conducting polyaniline based cell culture substrate for embryonic stem cells and embryoid bodies
JF  - RSC Advances
VL  - 5
IS  - 62
SP  - 50328-50335
EP  - 50328-50335
PB  - Royal Society of Chemistry
SN  - 20462069
KW  - Polyaniline
KW  - stem cells
UR  - http://pubs.rsc.org/en/Content/ArticleLanding/2015/RA/C5RA07504A#!divAbstract
L2  - http://pubs.rsc.org/en/Content/ArticleLanding/2015/RA/C5RA07504A#!divAbstract
N2  - In this work, thin films consisting of electrically conducting polyaniline (PANI) and poly(2-acrylamido-2-methyl-1-propanesulfonate) (PAMPSA) have been used as cell culture substrates for embryonic stem cells (ESC) and embryoid bodies (EMB). The PANI–PAMPSA films having fibrillar morphology were electrochemically polymerized in a single-step by cyclic voltammetry from an aqueous solution containing aniline and PAMPSA. UV-visible spectroscopy showed that the PANI films were electrically conducting still at pH 10. This makes them suitable for tissue engineering applications operating at physiological pH, in contrast to the commonly used PANI hydrochloride films which loose their electrical conductivity at pH 4 and higher. Our results reveal that the PANI–PAMPSA films allow only for limited ESC adhesion and growth. The inhibition of the EMB growth and adhesion on the PANI–PAMPSA surface in serum-free medium indicates that it can be used as a cell-selective substrate for the growth of only some specific differentiated EMB cell types.
ER  -

BOBER, Patrycja; Petr HUMPOLÍČEK; Jiří PACHERNÍK; Jaroslav STEJSKAL a Tom LINDFORS. Conducting polyaniline based cell culture substrate for embryonic stem cells and embryoid bodies. \textit{RSC Advances}. Cambridge: Royal Society of Chemistry, 2015, roč.~5, č.~62, s.~50328-50335. ISSN~2046-2069. Dostupné z: https://doi.org/10.1039/C5RA07504A.

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