PHYLOGENETIC ANALYSIS OF CULTIVATION-RESISTANT TERRESTRIAL
CYANOBACTERIA WITH MASSIVE SHEATHS (STIGONEMA SPP. AND
PETALONEMA ALATUM, NOSTOCALES, CYANOBACTERIA) USING SINGLE-CELL
AND FILAMENT SEQUENCING OF ENVIRONMENTAL SAMPLES

J 2015

PHYLOGENETIC ANALYSIS OF CULTIVATION-RESISTANT TERRESTRIAL CYANOBACTERIA WITH MASSIVE SHEATHS (STIGONEMA SPP. AND PETALONEMA ALATUM, NOSTOCALES, CYANOBACTERIA) USING SINGLE-CELL AND FILAMENT SEQUENCING OF ENVIRONMENTAL SAMPLES

MAREŠ, Jan; Lara YANNICK; Iva DADÁKOVÁ; Tomáš HAUER; Bohuslav UHER et al.

Základní údaje

Originální název

PHYLOGENETIC ANALYSIS OF CULTIVATION-RESISTANT TERRESTRIAL CYANOBACTERIA WITH MASSIVE SHEATHS (STIGONEMA SPP. AND PETALONEMA ALATUM, NOSTOCALES, CYANOBACTERIA) USING SINGLE-CELL AND FILAMENT SEQUENCING OF ENVIRONMENTAL SAMPLES

Autoři

MAREŠ, Jan; Lara YANNICK; Iva DADÁKOVÁ; Tomáš HAUER; Bohuslav UHER; Annick WILMOTTE a Jan KAŠTOVSKÝ

Vydání

Journal of Phycology, Hoboken, Wiley-Blackwell, 2015, 0022-3646

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.536

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/15:00087123

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova česky

16S rRNA gen; cyanobacteria; morfologie; Petalonema; fylogeneze; single cell; single filament; Stigonema; taxonomie

Klíčová slova anglicky

16S rRNA gene;cyanobacteria;morphology; Petalonema ;phylogeny;single cell;single filament; Stigonema ;taxonomy

Štítky

AKR, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 7. 3. 2018 15:48, Mgr. Lucie Jarošová, DiS.

Anotace

V originále

Molecular assessment of a large portion of traditional cyanobacterial taxa has been hindered by the failure to isolate and grow them in culture. In this study, we developed an optimized protocol for single cell/filament isolation and 16S rRNA gene sequencing of terrestrial cyanobacteria with large mucilaginous sheaths, and applied it to determine the phylogenetic position of typical members of the genera Petalonema and Stigonema. A methodology based on a glass-capillary isolation technique and a semi-nested PCR protocol enabled reliable sequencing of the 16S rRNA gene from all samples analysed. Ten samples covering 7 species of Stigonema from Europe, North and Central America, and Hawaii, and the type species of Petalonema from Slovakia were sequenced. Contrary to some previous studies, which proposed a relation to heteropolar nostocalean cyanobacteria, Petalonema appeared to belong to the family Scytonemataceae. Analysis of Stigonema specimens recovered a unique coherent phylogenetic cluster, substantially broadening our knowledge of the molecular diversity within this genus. Neither the uni- to biseriate species nor the multiseriate species formed monophyletic subclusters within the genus.Typical multiseriate species of Stigonema clustered in a phylogenetic branch derived from uni- to biseriate S. ocellatum Thuret ex Bornet & Flahault in our analysis, suggesting that species with more complex thalli may have evolved from the more simple ones. We propose the technique tested in this study as a promising tool for a future revision of the molecular taxonomy in cyanobacteria.This article is protected by copyright. All rights reserved.

Citovat

MAREŠ, Jan; Lara YANNICK; Iva DADÁKOVÁ; Tomáš HAUER; Bohuslav UHER; Annick WILMOTTE a Jan KAŠTOVSKÝ. PHYLOGENETIC ANALYSIS OF CULTIVATION-RESISTANT TERRESTRIAL CYANOBACTERIA WITH MASSIVE SHEATHS (STIGONEMA SPP. AND PETALONEMA ALATUM, NOSTOCALES, CYANOBACTERIA) USING SINGLE-CELL AND FILAMENT SEQUENCING OF ENVIRONMENTAL SAMPLES. Journal of Phycology. Hoboken: Wiley-Blackwell, 2015, roč. 51, č. 2, s. 288-297. ISSN 0022-3646. Dostupné z: https://doi.org/10.1111/jpy.12273.

@article{1340485,
   author = {Mareš, Jan and Yannick, Lara and Dadáková, Iva and Hauer, Tomáš and Uher, Bohuslav and Wilmotte, Annick and Kaštovský, Jan},
   article_location = {Hoboken},
   article_number = {2},
   doi = {https://doi.org/10.1111/jpy.12273},
   keywords = {16S rRNA gene;cyanobacteria;morphology; Petalonema ;phylogeny;single cell;single filament; Stigonema ;taxonomy},
   language = {eng},
   issn = {0022-3646},
   journal = {Journal of Phycology},
   title = {PHYLOGENETIC ANALYSIS OF CULTIVATION-RESISTANT TERRESTRIAL CYANOBACTERIA WITH MASSIVE SHEATHS (STIGONEMA SPP. AND PETALONEMA ALATUM, NOSTOCALES, CYANOBACTERIA) USING SINGLE-CELL AND FILAMENT SEQUENCING OF ENVIRONMENTAL SAMPLES},
   volume = {51},
   year = {2015}
}

TY  - JOUR
ID  - 1340485
AU  - Mareš, Jan - Yannick, Lara - Dadáková, Iva - Hauer, Tomáš - Uher, Bohuslav - Wilmotte, Annick - Kaštovský, Jan
PY  - 2015
TI  - PHYLOGENETIC ANALYSIS OF CULTIVATION-RESISTANT TERRESTRIAL CYANOBACTERIA WITH MASSIVE SHEATHS (STIGONEMA SPP. AND PETALONEMA ALATUM, NOSTOCALES, CYANOBACTERIA) USING SINGLE-CELL AND FILAMENT SEQUENCING OF ENVIRONMENTAL SAMPLES
JF  - Journal of Phycology
VL  - 51
IS  - 2
SP  - 288-297
EP  - 288-297
PB  - Wiley-Blackwell
SN  - 00223646
KW  - 16S rRNA gene;cyanobacteria;morphology
KW  - Petalonema ;phylogeny;single cell;single filament
KW  - Stigonema ;taxonomy
N2  - Molecular assessment of a large portion of traditional cyanobacterial taxa has been hindered by the failure to isolate and grow them in culture. In this study, we developed an optimized protocol for single cell/filament isolation and 16S rRNA gene sequencing of terrestrial cyanobacteria with large mucilaginous sheaths, and applied it to determine the phylogenetic position of typical members of the genera Petalonema and Stigonema. A methodology based on a glass-capillary isolation technique and a semi-nested PCR protocol enabled reliable sequencing of the 16S rRNA gene from all samples analysed. Ten samples covering 7 species of Stigonema from Europe, North and Central America, and Hawaii, and the type species of Petalonema from Slovakia were sequenced. Contrary to some previous studies, which proposed a relation to heteropolar nostocalean cyanobacteria, Petalonema appeared to belong to the family Scytonemataceae. Analysis of Stigonema specimens recovered a unique coherent phylogenetic cluster, substantially broadening our knowledge of the molecular diversity within this genus. Neither the uni- to biseriate species nor the multiseriate species formed monophyletic subclusters within the genus.Typical multiseriate species of Stigonema clustered in a phylogenetic branch derived from uni- to biseriate S. ocellatum Thuret ex Bornet & Flahault in our analysis, suggesting that species with more complex thalli may have evolved from the more simple ones. We propose the technique tested in this study as a promising tool for a future revision of the molecular taxonomy in cyanobacteria.This article is protected by copyright. All rights reserved.
ER  -

MAREŠ, Jan; Lara YANNICK; Iva DADÁKOVÁ; Tomáš HAUER; Bohuslav UHER; Annick WILMOTTE a Jan KAŠTOVSKÝ. PHYLOGENETIC ANALYSIS OF CULTIVATION-RESISTANT TERRESTRIAL CYANOBACTERIA WITH MASSIVE SHEATHS (STIGONEMA SPP. AND PETALONEMA ALATUM, NOSTOCALES, CYANOBACTERIA) USING SINGLE-CELL AND FILAMENT SEQUENCING OF ENVIRONMENTAL SAMPLES. \textit{Journal of Phycology}. Hoboken: Wiley-Blackwell, 2015, roč.~51, č.~2, s.~288-297. ISSN~0022-3646. Dostupné z: https://doi.org/10.1111/jpy.12273.

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