Influence of Culture Media on Microbial Fingerprints Using
Raman Spectroscopy

J 2015

Influence of Culture Media on Microbial Fingerprints Using Raman Spectroscopy

MLYNÁRIKOVÁ, Katarína; Ota SAMEK; Silvie BERNATOVÁ; Filip RŮŽIČKA; Jan JEŽEK et al.

Základní údaje

Originální název

Influence of Culture Media on Microbial Fingerprints Using Raman Spectroscopy

Autoři

MLYNÁRIKOVÁ, Katarína; Ota SAMEK; Silvie BERNATOVÁ; Filip RŮŽIČKA ; Jan JEŽEK; Andrea HÁRONIKOVÁ; Martin ŠILER; Pavel ZEMÁNEK a Veronika HOLÁ

Vydání

Sensors, Basel, MDPI AG, 2015, 1424-8220

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.033

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/15:00087136

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

Raman spectroscopy; bacteria; yeasts; culture media

Štítky

EL OK

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 29. 3. 2016 10:46, Soňa Böhmová

Anotace

V originále

Raman spectroscopy has a broad range of applications across numerous scientific fields, including microbiology. Our work here monitors the influence of culture media on the Raman spectra of clinically important microorganisms (Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis and Candida albicans). Choosing an adequate medium may enhance the reproducibility of the method as well as simplifying the data processing and the evaluation. We tested four different media per organism depending on the nutritional requirements and clinical usage directly on a Petri dish. Some of the media have a significant influence on the microbial fingerprint (Roosvelt-Park Institute Medium, CHROMagar) and should not be used for the acquisition of Raman spectra. It was found that the most suitable medium for microbiological experiments regarding these organisms was Mueller-Hinton agar.

Návaznosti

LG13011, projekt VaV

Název: Zapojení České republiky do aktivit Americké společnosti pro mikrobiologii (ASM) (Akronym: zcrdaasm)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Zapojení České republiky do aktivit Americké společnosti pro mikrobiologii (ASM)

Citovat

MLYNÁRIKOVÁ, Katarína; Ota SAMEK; Silvie BERNATOVÁ; Filip RŮŽIČKA; Jan JEŽEK; Andrea HÁRONIKOVÁ; Martin ŠILER; Pavel ZEMÁNEK a Veronika HOLÁ. Influence of Culture Media on Microbial Fingerprints Using Raman Spectroscopy. Sensors. Basel: MDPI AG, 2015, roč. 15, č. 11, s. 29635-29647. ISSN 1424-8220. Dostupné z: https://doi.org/10.3390/s151129635.

@article{1340812,
   author = {Mlynáriková, Katarína and Samek, Ota and Bernatová, Silvie and Růžička, Filip and Ježek, Jan and Hároniková, Andrea and Šiler, Martin and Zemánek, Pavel and Holá, Veronika},
   article_location = {Basel},
   article_number = {11},
   doi = {https://doi.org/10.3390/s151129635},
   keywords = {Raman spectroscopy; bacteria; yeasts; culture media},
   language = {eng},
   issn = {1424-8220},
   journal = {Sensors},
   title = {Influence of Culture Media on Microbial Fingerprints Using Raman Spectroscopy},
   volume = {15},
   year = {2015}
}

TY  - JOUR
ID  - 1340812
AU  - Mlynáriková, Katarína - Samek, Ota - Bernatová, Silvie - Růžička, Filip - Ježek, Jan - Hároniková, Andrea - Šiler, Martin - Zemánek, Pavel - Holá, Veronika
PY  - 2015
TI  - Influence of Culture Media on Microbial Fingerprints Using Raman Spectroscopy
JF  - Sensors
VL  - 15
IS  - 11
SP  - 29635-29647
EP  - 29635-29647
PB  - MDPI AG
SN  - 14248220
KW  - Raman spectroscopy
KW  - bacteria
KW  - yeasts
KW  - culture media
N2  - Raman spectroscopy has a broad range of applications across numerous scientific fields, including microbiology. Our work here monitors the influence of culture media on the Raman spectra of clinically important microorganisms (Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis and Candida albicans). Choosing an adequate medium may enhance the reproducibility of the method as well as simplifying the data processing and the evaluation. We tested four different media per organism depending on the nutritional requirements and clinical usage directly on a Petri dish. Some of the media have a significant influence on the microbial fingerprint (Roosvelt-Park Institute Medium, CHROMagar) and should not be used for the acquisition of Raman spectra. It was found that the most suitable medium for microbiological experiments regarding these organisms was Mueller-Hinton agar.
ER  -

MLYNÁRIKOVÁ, Katarína; Ota SAMEK; Silvie BERNATOVÁ; Filip RŮŽIČKA; Jan JEŽEK; Andrea HÁRONIKOVÁ; Martin ŠILER; Pavel ZEMÁNEK a Veronika HOLÁ. Influence of Culture Media on Microbial Fingerprints Using Raman Spectroscopy. \textit{Sensors}. Basel: MDPI AG, 2015, roč.~15, č.~11, s.~29635-29647. ISSN~1424-8220. Dostupné z: https://doi.org/10.3390/s151129635.

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