Mast cell stabilization with sodium cromoglycate modulates
pulmonary vessel wall remodeling during four-day hypoxia in
rats

J 2015

Mast cell stabilization with sodium cromoglycate modulates pulmonary vessel wall remodeling during four-day hypoxia in rats

NOVOTNÝ, Tomáš; Jakub KREJČÍ; Jana MALÍKOVÁ; Vojtěch ŠVEHLÍK; Roman WASSERBAUER et al.

Základní údaje

Originální název

Mast cell stabilization with sodium cromoglycate modulates pulmonary vessel wall remodeling during four-day hypoxia in rats

Autoři

NOVOTNÝ, Tomáš; Jakub KREJČÍ; Jana MALÍKOVÁ; Vojtěch ŠVEHLÍK; Roman WASSERBAUER; Jiří UHLÍK a Luděk VAJNER

Vydání

Experimental Lung Research, London, Informa Healthcare, 2015, 0190-2148

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30203 Respiratory systems

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.374

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/15:00087223

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

hypoxic pulmonary hypertension; matrix metalloproteinase 13; morphometry; normobaric hypoxia

Štítky

EL OK

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 5. 4. 2016 09:32, Ing. Mgr. Věra Pospíšilíková

Anotace

V originále

Aim of the study: In rats, the environment with low content of oxygen induces hypoxic pulmonary hypertension. Remodeling of pulmonary resistance arteries is particularly triggered by the mast cell degranulation products, e.g., rodent-like interstitial collagenase (matrix metalloproteinase 13). Administration of sodium cromoglycate leads to stabilization of mast cell granules, and thus to the modified remodeling process. Materials and Methods: During four-day hypoxia, we treated rats with sodium cromoglycate. Pulmonary vascular remodeling was assessed as well as counts of periarterial pulmonary mast cells, both total and matrix metalloproteinase 13-positive ones. Results: Four-day hypoxia induced remodeling of both resistance arteries and large conduit arteries. We have found increase in the tunica media thickness of resistance arteries. Tunica adventitia thickness of both resistance arteries and large conduit arteries with a diameter of over 300 Am increased as well; the latter ones revealed increase in the number of vasa vasorum in their walls. Mast cell stabilization suppressed hypoxic pulmonary vascular remodeling in resistance pulmonary arteries. Four-day hypoxia led to changes in distribution of toluidine blue-detected and MMP-13 positive periarterial mast cells; this redistribution was also influenced by the administration of sodium cromoglycate. Conclusions: The number of pulmonary periarterial mast cells seemingly decreases during hypoxia due to their degranulation, which disables their identification. Large conduit arteries do not affect final blood pressure in the pulmonary vascular bed; however, their structure changes substantially under hypoxia. Such remodeling changes are not mediated by mast cell products only since they have occurred in spite of stabilization of mast cell granules.

Citovat

NOVOTNÝ, Tomáš; Jakub KREJČÍ; Jana MALÍKOVÁ; Vojtěch ŠVEHLÍK; Roman WASSERBAUER; Jiří UHLÍK a Luděk VAJNER. Mast cell stabilization with sodium cromoglycate modulates pulmonary vessel wall remodeling during four-day hypoxia in rats. Experimental Lung Research. London: Informa Healthcare, 2015, roč. 41, č. 5, s. 283-292. ISSN 0190-2148. Dostupné z: https://doi.org/10.3109/01902148.2015.1018558.

@article{1341603,
   author = {Novotný, Tomáš and Krejčí, Jakub and Malíková, Jana and Švehlík, Vojtěch and Wasserbauer, Roman and Uhlík, Jiří and Vajner, Luděk},
   article_location = {London},
   article_number = {5},
   doi = {https://doi.org/10.3109/01902148.2015.1018558},
   keywords = {hypoxic pulmonary hypertension; matrix metalloproteinase 13; morphometry; normobaric hypoxia},
   language = {eng},
   issn = {0190-2148},
   journal = {Experimental Lung Research},
   title = {Mast cell stabilization with sodium cromoglycate modulates pulmonary vessel wall remodeling during four-day hypoxia in rats},
   volume = {41},
   year = {2015}
}

TY  - JOUR
ID  - 1341603
AU  - Novotný, Tomáš - Krejčí, Jakub - Malíková, Jana - Švehlík, Vojtěch - Wasserbauer, Roman - Uhlík, Jiří - Vajner, Luděk
PY  - 2015
TI  - Mast cell stabilization with sodium cromoglycate modulates pulmonary vessel wall remodeling during four-day hypoxia in rats
JF  - Experimental Lung Research
VL  - 41
IS  - 5
SP  - 283-292
EP  - 283-292
PB  - Informa Healthcare
SN  - 01902148
KW  - hypoxic pulmonary hypertension
KW  - matrix metalloproteinase 13
KW  - morphometry
KW  - normobaric hypoxia
N2  - Aim of the study: In rats, the environment with low content of oxygen induces hypoxic pulmonary hypertension. Remodeling of pulmonary resistance arteries is particularly triggered by the mast cell degranulation products, e.g., rodent-like interstitial collagenase (matrix metalloproteinase 13). Administration of sodium cromoglycate leads to stabilization of mast cell granules, and thus to the modified remodeling process. Materials and Methods: During four-day hypoxia, we treated rats with sodium cromoglycate. Pulmonary vascular remodeling was assessed as well as counts of periarterial pulmonary mast cells, both total and matrix metalloproteinase 13-positive ones. Results: Four-day hypoxia induced remodeling of both resistance arteries and large conduit arteries. We have found increase in the tunica media thickness of resistance arteries. Tunica adventitia thickness of both resistance arteries and large conduit arteries with a diameter of over 300 Am increased as well; the latter ones revealed increase in the number of vasa vasorum in their walls. Mast cell stabilization suppressed hypoxic pulmonary vascular remodeling in resistance pulmonary arteries. Four-day hypoxia led to changes in distribution of toluidine blue-detected and MMP-13 positive periarterial mast cells; this redistribution was also influenced by the administration of sodium cromoglycate. Conclusions: The number of pulmonary periarterial mast cells seemingly decreases during hypoxia due to their degranulation, which disables their identification. Large conduit arteries do not affect final blood pressure in the pulmonary vascular bed; however, their structure changes substantially under hypoxia. Such remodeling changes are not mediated by mast cell products only since they have occurred in spite of stabilization of mast cell granules.
ER  -

NOVOTNÝ, Tomáš; Jakub KREJČÍ; Jana MALÍKOVÁ; Vojtěch ŠVEHLÍK; Roman WASSERBAUER; Jiří UHLÍK a Luděk VAJNER. Mast cell stabilization with sodium cromoglycate modulates pulmonary vessel wall remodeling during four-day hypoxia in rats. \textit{Experimental Lung Research}. London: Informa Healthcare, 2015, roč.~41, č.~5, s.~283-292. ISSN~0190-2148. Dostupné z: https://doi.org/10.3109/01902148.2015.1018558.

Přehled o publikaci