Rapid assignment of malting barley varieties by matrix-assisted
laser desorption-ionisation - Time-of-flight mass spectrometry

J 2016

Rapid assignment of malting barley varieties by matrix-assisted laser desorption-ionisation - Time-of-flight mass spectrometry

ŠEDO, Ondrej, Michal KOŘÁN, Michaela JAKEŠOVÁ, Renata MIKULÍKOVÁ, Michal BOHÁČ et. al.

Základní údaje

Originální název

Rapid assignment of malting barley varieties by matrix-assisted laser desorption-ionisation - Time-of-flight mass spectrometry

Autoři

ŠEDO, Ondrej (203 Česká republika, domácí), Michal KOŘÁN (203 Česká republika), Michaela JAKEŠOVÁ (203 Česká republika, domácí), Renata MIKULÍKOVÁ (203 Česká republika), Michal BOHÁČ (203 Česká republika) a Zbyněk ZDRÁHAL (203 Česká republika, garant, domácí)

Vydání

Food Chemistry, Oxford, ELSEVIER SCI LTD, 2016, 0308-8146

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.529

Kód RIV

RIV/00216224:14740/16:00087891

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1016/j.foodchem.2016.03.056

UT WoS

000373054500017

Klíčová slova anglicky

Barley; Malting; MALDI-TOF MS; Proteins

Štítky

CF PROT, rivok

Změněno: 22. 2. 2017 11:09, Mgr. Eva Špillingová

Anotace

V originále

A method for discriminating malting barley varieties based on direct matrix-assisted laser desorption-ionisation -time-of-flight mass spectrometry (MALDI-TOF MS) fingerprinting of proteins was developed. Signals corresponding to hordeins were obtained by simple mixing of powdered barley grain with a MALDI matrix solution containing 12.5 mg mL(-1) of ferulic acid in an acetonitrile: water: formic acid 50:33:17 v/v/v mixture. Compared to previous attempts at MALDI-TOF mass spectrometric analysis of barley proteins, the extraction and fractionation steps were practically omitted, resulting in a significant reduction in analytical time and costs. The discriminatory power was examined on twenty malting barley varieties and the practicability of the method was tested on sixty barley samples acquired from Pilsner Urquell Brewery. The method is proposed as a rapid tool for variety assignment and purity determination of malting barley that may replace gel electrophoresis currently used for this purpose.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

GBP206/12/G151, projekt VaV

Název: Centrum nových přístupů k bioanalýze a molekulární diagnostice

LM2015043, projekt VaV

Název: Česká infrastruktura pro integrativní strukturní biologii (Akronym: CIISB)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech Infrastructure for Integrative Structural Biology

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

ŠEDO, Ondrej, Michal KOŘÁN, Michaela JAKEŠOVÁ, Renata MIKULÍKOVÁ, Michal BOHÁČ a Zbyněk ZDRÁHAL. Rapid assignment of malting barley varieties by matrix-assisted laser desorption-ionisation - Time-of-flight mass spectrometry. Food Chemistry. Oxford: ELSEVIER SCI LTD, 2016, roč. 206, September, s. 124-130. ISSN 0308-8146. Dostupné z: https://dx.doi.org/10.1016/j.foodchem.2016.03.056.

@article{1343703,
   author = {Šedo, Ondrej and Kořán, Michal and Jakešová, Michaela and Mikulíková, Renata and Boháč, Michal and Zdráhal, Zbyněk},
   article_location = {Oxford},
   article_number = {September},
   doi = {http://dx.doi.org/10.1016/j.foodchem.2016.03.056},
   keywords = {Barley; Malting; MALDI-TOF MS; Proteins},
   language = {eng},
   issn = {0308-8146},
   journal = {Food Chemistry},
   title = {Rapid assignment of malting barley varieties by matrix-assisted laser desorption-ionisation - Time-of-flight mass spectrometry},
   url = {http://www.sciencedirect.com/science/article/pii/S0308814616304095},
   volume = {206},
   year = {2016}
}

TY  - JOUR
ID  - 1343703
AU  - Šedo, Ondrej - Kořán, Michal - Jakešová, Michaela - Mikulíková, Renata - Boháč, Michal - Zdráhal, Zbyněk
PY  - 2016
TI  - Rapid assignment of malting barley varieties by matrix-assisted laser desorption-ionisation - Time-of-flight mass spectrometry
JF  - Food Chemistry
VL  - 206
IS  - September
SP  - 124-130
EP  - 124-130
PB  - ELSEVIER SCI LTD
SN  - 03088146
KW  - Barley
KW  - Malting
KW  - MALDI-TOF MS
KW  - Proteins
UR  - http://www.sciencedirect.com/science/article/pii/S0308814616304095
L2  - http://www.sciencedirect.com/science/article/pii/S0308814616304095
N2  - A method for discriminating malting barley varieties based on direct matrix-assisted laser desorption-ionisation -time-of-flight mass spectrometry (MALDI-TOF MS) fingerprinting of proteins was developed. Signals corresponding to hordeins were obtained by simple mixing of powdered barley grain with a MALDI matrix solution containing 12.5 mg mL(-1) of ferulic acid in an acetonitrile: water: formic acid 50:33:17 v/v/v mixture. Compared to previous attempts at MALDI-TOF mass spectrometric analysis of barley proteins, the extraction and fractionation steps were practically omitted, resulting in a significant reduction in analytical time and costs. The discriminatory power was examined on twenty malting barley varieties and the practicability of the method was tested on sixty barley samples acquired from Pilsner Urquell Brewery. The method is proposed as a rapid tool for variety assignment and purity determination of malting barley that may replace gel electrophoresis currently used for this purpose.
ER  -

ŠEDO, Ondrej, Michal KOŘÁN, Michaela JAKEŠOVÁ, Renata MIKULÍKOVÁ, Michal BOHÁČ a Zbyněk ZDRÁHAL. Rapid assignment of malting barley varieties by matrix-assisted laser desorption-ionisation - Time-of-flight mass spectrometry. \textit{Food Chemistry}. Oxford: ELSEVIER SCI LTD, 2016, roč.~206, September, s.~124-130. ISSN~0308-8146. Dostupné z: https://dx.doi.org/10.1016/j.foodchem.2016.03.056.

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