Effects of the soil microbial community on mobile proportions
and speciation of mercury (Hg) in contaminated soil

J 2016

Effects of the soil microbial community on mobile proportions and speciation of mercury (Hg) in contaminated soil

SZÁKOVÁ, Jiřina; Jitka HAVLÍČKOVÁ; Adéla ŠÍPKOVÁ; Jiří GABRIEL; Karel ŠVEC et al.

Základní údaje

Originální název

Effects of the soil microbial community on mobile proportions and speciation of mercury (Hg) in contaminated soil

Autoři

Vydání

Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering, Philadelphia, Taylor & Francis, 2016, 1093-4529

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.425

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/16:00087915

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

mercury; microbial community; mobility; rhizobox; speciation

Štítky

AKR, EL OK, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 15. 12. 2016 13:11, Ing. Mgr. Věra Pospíšilíková

Anotace

V originále

The precise characterization of the behavior of individual microorganisms in the presence of increased mercury contents in soil is necessary for better elucidation of the fate of mercury in the soil environment. In our investigation, resistant bacterial strains isolated from two mercury contaminated soils, represented by Paenibacillus alginolyticus, Burkholderia glathei, Burkholderia sp., and Pseudomonas sp., were used. Two differently contaminated soils (0.5 and 7mg kg(-1) total mercury) were chosen. Preliminary soil analysis showed the presence of methylmercury and phenylmercury with the higher soil mercury level. Modified rhizobox experiments were performed to assess the ability of mercury accumulating strains to deplete the mobile and mobilizable mercury portions in the soil by modification; microbial agar cultures were used rather than the plant root zone. A sequential extraction procedure was performed to release the following mercury fractions: water soluble, extracted in acidic conditions, bound to humic substances, elemental, and bound to complexes, HgS and residual. Inductively coupled plasma mass spectrometry (ICP-MS) and a single-purpose atomic absorption spectrometer (AMA-254) were applied for mercury determination in the samples and extracts. Gas chromatography coupled to atomic fluorescence spectrometry (GC-AFS) was used for the determination of organomercury compounds. The analysis of the microbial community at the end of the experiment showed a 42% abundance of Paenibacillus sp. followed by Acetivibrio sp., Brevibacillus sp., Cohnella sp., Lysinibacillus sp., and Clostridium sp. not exceeding 2% abundance. The results suggest importance of Paenibacillus sp. in Hg transformation processes. This genus should be tested for potential bioremediation use in further research.

Návaznosti

GAP503/12/0682, projekt VaV

Název: Transformace sloučenin rtuti půdní mikroflórou: možné využití pro bioremediační technologie

Investor: Grantová agentura ČR, Transformace sloučenin rtuti půdní mikroflórou: možné využití pro bioremediační technologie

Citovat

SZÁKOVÁ, Jiřina; Jitka HAVLÍČKOVÁ; Adéla ŠÍPKOVÁ; Jiří GABRIEL; Karel ŠVEC; Petr BALDRIAN; Jiřina SYSALOVÁ; Pavel COUFALÍK; Rostislav ČERVENKA; Ondřej ZVĚŘINA; Josef KOMÁREK a Pavel TLUSTOŠ. Effects of the soil microbial community on mobile proportions and speciation of mercury (Hg) in contaminated soil. Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering. Philadelphia: Taylor & Francis, 2016, roč. 51, č. 4, s. 364-370. ISSN 1093-4529. Dostupné z: https://doi.org/10.1080/10934529.2015.1109413.

@article{1344324,
   author = {Száková, Jiřina and Havlíčková, Jitka and Šípková, Adéla and Gabriel, Jiří and Švec, Karel and Baldrian, Petr and Sysalová, Jiřina and Coufalík, Pavel and Červenka, Rostislav and Zvěřina, Ondřej and Komárek, Josef and Tlustoš, Pavel},
   article_location = {Philadelphia},
   article_number = {4},
   doi = {https://doi.org/10.1080/10934529.2015.1109413},
   keywords = {mercury; microbial community; mobility; rhizobox; speciation},
   language = {eng},
   issn = {1093-4529},
   journal = {Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering},
   title = {Effects of the soil microbial community on mobile proportions and speciation of mercury (Hg) in contaminated soil},
   volume = {51},
   year = {2016}
}

TY  - JOUR
ID  - 1344324
AU  - Száková, Jiřina - Havlíčková, Jitka - Šípková, Adéla - Gabriel, Jiří - Švec, Karel - Baldrian, Petr - Sysalová, Jiřina - Coufalík, Pavel - Červenka, Rostislav - Zvěřina, Ondřej - Komárek, Josef - Tlustoš, Pavel
PY  - 2016
TI  - Effects of the soil microbial community on mobile proportions and speciation of mercury (Hg) in contaminated soil
JF  - Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering
VL  - 51
IS  - 4
SP  - 364-370
EP  - 364-370
PB  - Taylor & Francis
SN  - 10934529
KW  - mercury
KW  - microbial community
KW  - mobility
KW  - rhizobox
KW  - speciation
N2  - The precise characterization of the behavior of individual microorganisms in the presence of increased mercury contents in soil is necessary for better elucidation of the fate of mercury in the soil environment. In our investigation, resistant bacterial strains isolated from two mercury contaminated soils, represented by Paenibacillus alginolyticus, Burkholderia glathei, Burkholderia sp., and Pseudomonas sp., were used. Two differently contaminated soils (0.5 and 7mg kg(-1) total mercury) were chosen. Preliminary soil analysis showed the presence of methylmercury and phenylmercury with the higher soil mercury level. Modified rhizobox experiments were performed to assess the ability of mercury accumulating strains to deplete the mobile and mobilizable mercury portions in the soil by modification; microbial agar cultures were used rather than the plant root zone. A sequential extraction procedure was performed to release the following mercury fractions: water soluble, extracted in acidic conditions, bound to humic substances, elemental, and bound to complexes, HgS and residual. Inductively coupled plasma mass spectrometry (ICP-MS) and a single-purpose atomic absorption spectrometer (AMA-254) were applied for mercury determination in the samples and extracts. Gas chromatography coupled to atomic fluorescence spectrometry (GC-AFS) was used for the determination of organomercury compounds. The analysis of the microbial community at the end of the experiment showed a 42% abundance of Paenibacillus sp. followed by Acetivibrio sp., Brevibacillus sp., Cohnella sp., Lysinibacillus sp., and Clostridium sp. not exceeding 2% abundance. The results suggest importance of Paenibacillus sp. in Hg transformation processes. This genus should be tested for potential bioremediation use in further research.
ER  -

SZÁKOVÁ, Jiřina; Jitka HAVLÍČKOVÁ; Adéla ŠÍPKOVÁ; Jiří GABRIEL; Karel ŠVEC; Petr BALDRIAN; Jiřina SYSALOVÁ; Pavel COUFALÍK; Rostislav ČERVENKA; Ondřej ZVĚŘINA; Josef KOMÁREK a Pavel TLUSTOŠ. Effects of the soil microbial community on mobile proportions and speciation of mercury (Hg) in contaminated soil. \textit{Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering}. Philadelphia: Taylor \&{} Francis, 2016, roč.~51, č.~4, s.~364-370. ISSN~1093-4529. Dostupné z: https://doi.org/10.1080/10934529.2015.1109413.

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