DNA-based identification and OspC serotyping in cultures of
Borrelia burgdorferi s.l. isolated from ticks collected in the
Moravia (Czech Republic)

J 2016

DNA-based identification and OspC serotyping in cultures of Borrelia burgdorferi s.l. isolated from ticks collected in the Moravia (Czech Republic)

NOREK, Adam, Lubomír JANDA a Alena ŽÁKOVSKÁ

Základní údaje

Originální název

DNA-based identification and OspC serotyping in cultures of Borrelia burgdorferi s.l. isolated from ticks collected in the Moravia (Czech Republic)

Autoři

NOREK, Adam (203 Česká republika, domácí), Lubomír JANDA (203 Česká republika, domácí) a Alena ŽÁKOVSKÁ (203 Česká republika, domácí)

Vydání

Journal of Vector Ecology, Wiley, 2016, 1081-1710

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.473

Kód RIV

RIV/00216224:14310/16:00089869

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1111/jvec.12209

UT WoS

000377219600023

Klíčová slova česky

Borrelia burgdorferi sensu lato OspC FlaB

Klíčová slova anglicky

Borrelia burgdorferi sensu lato OspC FlaB

Štítky

AKR, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 4. 4. 2017 12:03, Ing. Andrea Mikešková

Anotace

V originále

In Europe nine different genospecies responsible for the human form of the LD have been reported. Among those B. afzelii, B. garinii and B. burdgorferii s. s. are the most prevalent in the Czech Republic. Each genospecies can be further subdivided into specific genotypes depending on genetic marker used in analysis. In our study we used two different loci (flaB and ospC) to assign genospecies and OspC phylogenetic type to eighteen strains previously isolated from ticks collected at a locality Pisárky, a suburban park near the city of Brno (Czech Republic). The RFLP analysis revealed three different genospecies (B. afzelii, B. garinii and B. valaisiana). Three samples from collection contained more than one genospecies. For other fifteen strains nucleotide sequences of flaB and ospC were determined. The phylogenetic analysis assigned our isolates to B. garinii (12) and B. afzelii (3) respectively, which were further subdivided into seven distinct ospC groups. The most related OspC types were G2, G4, G5 (B. garinii) and A3 and A8 (B. afzelii).

Návaznosti

MUNI/E/0131/2009, interní kód MU

Název: Sekvenace kandidátního genu pro vakcínu, tj. genu pro OspC protein u českcýh izolátů kmenů patogenní Borrelia burgdorferi sensu lato, původce Lymeské borreliózy

Investor: Masarykova univerzita, Sekvenace kandidátního genu pro vakcínu, tj. genu pro OspC protein u českcýh izolátů kmenů patogenní Borrelia burgdorferi sensu lato, původce Lymeské borreliózy, Kat. E - Podpora výzkumné činnosti studentů v oborech lékařství, zdravotnictví, přírodovědy a informatiky - rozvojový projekt + specifický výzkum

Citovat

NOREK, Adam, Lubomír JANDA a Alena ŽÁKOVSKÁ. DNA-based identification and OspC serotyping in cultures of Borrelia burgdorferi s.l. isolated from ticks collected in the Moravia (Czech Republic). Journal of Vector Ecology. Wiley, 2016, roč. 41, č. 1, s. 172-178. ISSN 1081-1710. Dostupné z: https://dx.doi.org/10.1111/jvec.12209.

@article{1344729,
   author = {Norek, Adam and Janda, Lubomír and Žákovská, Alena},
   article_number = {1},
   doi = {http://dx.doi.org/10.1111/jvec.12209},
   keywords = {Borrelia burgdorferi sensu lato OspC FlaB},
   language = {eng},
   issn = {1081-1710},
   journal = {Journal of Vector Ecology},
   title = {DNA-based identification and OspC serotyping in cultures of Borrelia burgdorferi s.l. isolated from ticks collected in the Moravia (Czech Republic)},
   volume = {41},
   year = {2016}
}

TY  - JOUR
ID  - 1344729
AU  - Norek, Adam - Janda, Lubomír - Žákovská, Alena
PY  - 2016
TI  - DNA-based identification and OspC serotyping in cultures of Borrelia burgdorferi s.l. isolated from ticks collected in the Moravia (Czech Republic)
JF  - Journal of Vector Ecology
VL  - 41
IS  - 1
SP  - 172-178
EP  - 172-178
PB  - Wiley
SN  - 10811710
KW  - Borrelia burgdorferi sensu lato OspC FlaB
N2  - In Europe nine different genospecies responsible for the human form of the LD have been reported. Among those B. afzelii, B. garinii and B. burdgorferii s. s. are the most prevalent in the Czech Republic. Each genospecies can be further subdivided into specific genotypes depending on genetic marker used in analysis. In our study we used two different loci (flaB and ospC) to assign genospecies and OspC phylogenetic type to eighteen strains previously isolated from ticks collected at a locality Pisárky, a suburban park near the city of Brno (Czech Republic). The RFLP analysis revealed three different genospecies (B. afzelii, B. garinii and B. valaisiana). Three samples from collection contained more than one genospecies. For other fifteen strains nucleotide sequences of flaB and ospC were determined. The phylogenetic analysis assigned our isolates to B. garinii (12) and B. afzelii (3) respectively, which were further subdivided into seven distinct ospC groups. The most related OspC types were G2, G4, G5 (B. garinii) and A3 and A8 (B. afzelii).
ER  -

NOREK, Adam, Lubomír JANDA a Alena ŽÁKOVSKÁ. DNA-based identification and OspC serotyping in cultures of Borrelia burgdorferi s.l. isolated from ticks collected in the Moravia (Czech Republic). \textit{Journal of Vector Ecology}. Wiley, 2016, roč.~41, č.~1, s.~172-178. ISSN~1081-1710. Dostupné z: https://dx.doi.org/10.1111/jvec.12209.

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