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@article{1345764,
author = {Jarque Ortiz, Sergio and Bittner, Michal and Hilscherová, Klára},
article_location = {OXFORD},
article_number = {April},
doi = {http://dx.doi.org/10.1016/j.chemosphere.2016.01.038},
keywords = {Recombinant yeast assay; Freeze-drying; Estrogen; Androgen; Long-term; Ready-to-use},
language = {eng},
issn = {0045-6535},
journal = {Chemosphere},
title = {Freeze-drying as suitable method to achieve ready-to-use yeast biosensors for androgenic and estrogenic compounds},
url = {http://www.sciencedirect.com/science/article/pii/S0045653516300376},
volume = {148},
year = {2016}
}
TY - JOUR
ID - 1345764
AU - Jarque Ortiz, Sergio - Bittner, Michal - Hilscherová, Klára
PY - 2016
TI - Freeze-drying as suitable method to achieve ready-to-use yeast biosensors for androgenic and estrogenic compounds
JF - Chemosphere
VL - 148
IS - April
SP - 204-210
EP - 204-210
PB - Elsevier Science
SN - 00456535
KW - Recombinant yeast assay
KW - Freeze-drying
KW - Estrogen
KW - Androgen
KW - Long-term
KW - Ready-to-use
UR - http://www.sciencedirect.com/science/article/pii/S0045653516300376
L2 - http://www.sciencedirect.com/science/article/pii/S0045653516300376
N2 - Recombinant yeast assays (RYAS) have been proved to be a suitable tool for the fast screening of compounds with endocrine disrupting activities. However, ready-to-use versions more accessible to less equipped laboratories and field studies are scarce and far from optimal throughputs. Here, we have applied freeze-drying technology to optimize RYA for the fast assessment of environmental compounds with estrogenic and androgenic potencies. The effects of different cryoprotectants, initial optical density and long-term storage were evaluated. The study included detailed characterization of sensitivity, robustness and reproducibility of the new ready-to-use versions, as well as comparison with the standard assays. Freeze-dried RYAS showed similar dose-responses curves to their homolog standard assays, with Lowest Observed Effect Concentration (LOEC) and Median effective Concentration (EC50) of 1 nM and 7.5 nM for testosterone, and 0.05 nM and 0.5 nM for 17 beta-estradiol, respectively. Freeze-dried cells stored at 4 degrees C retained maximum sensitivity up to 2 months, while cells stored at -18 degrees C showed no decrease in sensitivity throughout the study (10 months). This ready-to-use RYA is easily accessible and may be potentially used for on-site applications.
ER -
JARQUE ORTIZ, Sergio, Michal BITTNER a Klára HILSCHEROVÁ. Freeze-drying as suitable method to achieve ready-to-use yeast biosensors for androgenic and estrogenic compounds. \textit{Chemosphere}. OXFORD: Elsevier Science, roč.~148, April, s.~204-210. ISSN~0045-6535. doi:10.1016/j.chemosphere.2016.01.038. 2016.
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