Electrochemical impedance spectroscopy based biosensor for
rapid detection of Salmonella

D 2016

Electrochemical impedance spectroscopy based biosensor for rapid detection of Salmonella

FARKA, Zdeněk; Tomáš JUŘÍK; Matěj PASTUCHA a Petr SKLÁDAL

Základní údaje

Originální název

Electrochemical impedance spectroscopy based biosensor for rapid detection of Salmonella

Autoři

FARKA, Zdeněk; Tomáš JUŘÍK; Matěj PASTUCHA a Petr SKLÁDAL

Vydání

Brno, XVI. Workshop of Physical Chemists and Electrochemists, od s. 66-69, 4 s. 2016

Nakladatel

Masaryk University

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

paměťový nosič (CD, DVD, flash disk)

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14740/16:00090128

Organizační jednotka

Středoevropský technologický institut

ISBN

978-80-210-8267-0

Klíčová slova anglicky

immunosensor; electrochemical impedance spectroscopy; food safety; Salmonella Typhimurium

Štítky

rivok

Změněno: 27. 3. 2017 10:51, Mgr. Eva Špillingová

Anotace

V originále

A label-free biosensor based on electrochemical impedance spectroscopy (EIS) was developed for the detection of Salmonella Typhimurium in milk. Specific antibody was immobilized on a screen-printed electrode, the electrode was incubated directly with the sample and binding of bacteria was measured as a change of impedance. Different procedures for sample treatment (combinations of heat and sonication) were tested and their impact on the assay performance was compared. Atomic force microscopy (AFM) was used to study the effect of the treatment on the cell shape and to confirm the specific binding of Salmonella to the sensing surface. The immunosensor allowed detection of 1×10^3 CFU·mL-1 in 20 min with negligible interference from other bacteria. A wide linear response was obtained in the range between 10^3 and 10^8 CFU·mL-1.

Návaznosti

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

FARKA, Zdeněk; Tomáš JUŘÍK; Matěj PASTUCHA a Petr SKLÁDAL. Electrochemical impedance spectroscopy based biosensor for rapid detection of Salmonella. In Libuše Trnková. XVI. Workshop of Physical Chemists and Electrochemists. Brno: Masaryk University, 2016, s. 66-69. ISBN 978-80-210-8267-0.

@inproceedings{1347080,
   author = {Farka, Zdeněk and Juřík, Tomáš and Pastucha, Matěj and Skládal, Petr},
   address = {Brno},
   booktitle = {XVI. Workshop of Physical Chemists and Electrochemists},
   editor = {Libuše Trnková},
   keywords = {immunosensor; electrochemical impedance spectroscopy; food safety; Salmonella Typhimurium},
   howpublished = {paměťový nosič},
   language = {eng},
   location = {Brno},
   isbn = {978-80-210-8267-0},
   pages = {66-69},
   publisher = {Masaryk University},
   title = {Electrochemical impedance spectroscopy based biosensor for rapid detection of Salmonella},
   year = {2016}
}

TY  - CONF
ID  - 1347080
AU  - Farka, Zdeněk - Juřík, Tomáš - Pastucha, Matěj - Skládal, Petr
PY  - 2016
TI  - Electrochemical impedance spectroscopy based biosensor for rapid detection of Salmonella
PB  - Masaryk University
CY  - Brno
SN  - 9788021082670
KW  - immunosensor
KW  - electrochemical impedance spectroscopy
KW  - food safety
KW  - Salmonella Typhimurium
N2  - A label-free biosensor based on electrochemical impedance spectroscopy (EIS) was developed for the detection of Salmonella Typhimurium in milk. Specific antibody was immobilized on a screen-printed electrode, the electrode was incubated directly with the sample and binding of bacteria was measured as a change of impedance. Different procedures for sample treatment (combinations of heat and sonication) were tested and their impact on the assay performance was compared. Atomic force microscopy (AFM) was used to study the effect of the treatment on the cell shape and to confirm the specific binding of Salmonella to the sensing surface. The immunosensor allowed detection of 1×10^3 CFU·mL-1 in 20 min with negligible interference from other bacteria. A wide linear response was obtained in the range between 10^3 and 10^8 CFU·mL-1.
ER  -

FARKA, Zdeněk; Tomáš JUŘÍK; Matěj PASTUCHA a Petr SKLÁDAL. Electrochemical impedance spectroscopy based biosensor for rapid detection of Salmonella. In Libuše Trnková. \textit{XVI. Workshop of Physical Chemists and Electrochemists}. Brno: Masaryk University, 2016, s.~66-69. ISBN~978-80-210-8267-0.

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