Competitive Upconversion-Linked Immunosorbent Assay for the
Sensitive Detection of Diclofenac

J 2016

Competitive Upconversion-Linked Immunosorbent Assay for the Sensitive Detection of Diclofenac

HLAVÁČEK, Antonín; Zdeněk FARKA; Maria HUEBNER; Veronika HORŇÁKOVÁ; Daniel NĚMEČEK et al.

Základní údaje

Originální název

Competitive Upconversion-Linked Immunosorbent Assay for the Sensitive Detection of Diclofenac

Autoři

HLAVÁČEK, Antonín; Zdeněk FARKA; Maria HUEBNER; Veronika HORŇÁKOVÁ; Daniel NĚMEČEK; Reinhard NIESSNER; Petr SKLÁDAL; Dietmar KNOPP a Hans Heiner GORRIS

Vydání

Analytical Chemistry, Washington, D.C., USA, American Chemical Society, 2016, 0003-2700

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 6.320

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14740/16:00090133

Organizační jednotka

Středoevropský technologický institut

Klíčová slova anglicky

antibody; bioconjugation; diclofenac; immunoassay; pharmaceutical micropollutant; photon-upconversion

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 15. 9. 2018 18:49, doc. Mgr. Zdeněk Farka, Ph.D.

Anotace

V originále

Photon-upconverting nanoparticles (UCNPs) emit light of shorter wavelength under near-infrared excitation and thus avoid optical background interference. We have exploited this unique photophysical feature to establish a sensitive competitive immunoassay for the detection of the pharmaceutical micropollutant diclofenac (DCF) in water. The so-called upconversion-linked immunosorbent assay (ULISA) was critically dependent on the design of the upconversion luminescent detection label. Silica-coated UCNPs (50 nm in diameter) exposing carboxyl groups on the surface were conjugated to a secondary anti-IgG antibody. We investigated the structure and monodispersity of the nanoconjugates in detail. Using a highly affine anti-DCF primary antibody, the optimized ULISA reached a detection limit of 0.05 ng DCF per mL. This performance came close to a conventional enzyme-linked immunosorbent assay (ELISA) without the need for an enzyme-mediated signal amplification step. The ULISA was further employed for analyzing drinking and surface water samples. The results were consistent with a conventional ELISA as well as liquid chromatography–mass spectrometry (LC–MS).

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

EE2.3.30.0009, projekt VaV

Název: Zaměstnáním čerstvých absolventů doktorského studia k vědecké excelenci

LD15023, projekt VaV

Název: Příprava diskrétních biokonjugátů photon-upconversion nanočástic

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Příprava diskrétních biokonjugátů photon-upconversion nanočástic

Citovat

HLAVÁČEK, Antonín; Zdeněk FARKA; Maria HUEBNER; Veronika HORŇÁKOVÁ; Daniel NĚMEČEK; Reinhard NIESSNER; Petr SKLÁDAL; Dietmar KNOPP a Hans Heiner GORRIS. Competitive Upconversion-Linked Immunosorbent Assay for the Sensitive Detection of Diclofenac. Analytical Chemistry. Washington, D.C., USA: American Chemical Society, 2016, roč. 88, č. 11, s. 6011-6017. ISSN 0003-2700. Dostupné z: https://doi.org/10.1021/acs.analchem.6b01083.

@article{1347122,
   author = {Hlaváček, Antonín and Farka, Zdeněk and Huebner, Maria and Horňáková, Veronika and Němeček, Daniel and Niessner, Reinhard and Skládal, Petr and Knopp, Dietmar and Gorris, Hans Heiner},
   article_location = {Washington, D.C., USA},
   article_number = {11},
   doi = {https://doi.org/10.1021/acs.analchem.6b01083},
   keywords = {antibody; bioconjugation; diclofenac; immunoassay; pharmaceutical micropollutant; photon-upconversion},
   language = {eng},
   issn = {0003-2700},
   journal = {Analytical Chemistry},
   title = {Competitive Upconversion-Linked Immunosorbent Assay for the Sensitive Detection of Diclofenac},
   url = {http://pubs.acs.org/doi/abs/10.1021/acs.analchem.6b01083},
   volume = {88},
   year = {2016}
}

TY  - JOUR
ID  - 1347122
AU  - Hlaváček, Antonín - Farka, Zdeněk - Huebner, Maria - Horňáková, Veronika - Němeček, Daniel - Niessner, Reinhard - Skládal, Petr - Knopp, Dietmar - Gorris, Hans Heiner
PY  - 2016
TI  - Competitive Upconversion-Linked Immunosorbent Assay for the Sensitive Detection of Diclofenac
JF  - Analytical Chemistry
VL  - 88
IS  - 11
SP  - 6011-6017
EP  - 6011-6017
PB  - American Chemical Society
SN  - 00032700
KW  - antibody
KW  - bioconjugation
KW  - diclofenac
KW  - immunoassay
KW  - pharmaceutical micropollutant
KW  - photon-upconversion
UR  - http://pubs.acs.org/doi/abs/10.1021/acs.analchem.6b01083
L2  - http://pubs.acs.org/doi/abs/10.1021/acs.analchem.6b01083
N2  - Photon-upconverting nanoparticles (UCNPs) emit light of shorter wavelength under near-infrared excitation and thus avoid optical background interference. We have exploited this unique photophysical feature to establish a sensitive competitive immunoassay for the detection of the pharmaceutical micropollutant diclofenac (DCF) in water. The so-called upconversion-linked immunosorbent assay (ULISA) was critically dependent on the design of the upconversion luminescent detection label. Silica-coated UCNPs (50 nm in diameter) exposing carboxyl groups on the surface were conjugated to a secondary anti-IgG antibody. We investigated the structure and monodispersity of the nanoconjugates in detail. Using a highly affine anti-DCF primary antibody, the optimized ULISA reached a detection limit of 0.05 ng DCF per mL. This performance came close to a conventional enzyme-linked immunosorbent assay (ELISA) without the need for an enzyme-mediated signal amplification step. The ULISA was further employed for analyzing drinking and surface water samples. The results were consistent with a conventional ELISA as well as liquid chromatography–mass spectrometry (LC–MS).
ER  -

HLAVÁČEK, Antonín; Zdeněk FARKA; Maria HUEBNER; Veronika HORŇÁKOVÁ; Daniel NĚMEČEK; Reinhard NIESSNER; Petr SKLÁDAL; Dietmar KNOPP a Hans Heiner GORRIS. Competitive Upconversion-Linked Immunosorbent Assay for the Sensitive Detection of Diclofenac. \textit{Analytical Chemistry}. Washington, D.C., USA: American Chemical Society, 2016, roč.~88, č.~11, s.~6011-6017. ISSN~0003-2700. Dostupné z: https://doi.org/10.1021/acs.analchem.6b01083.

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