Competitive Upconversion-Linked Immunosorbent Assay for the
Sensitive Detection of Diclofenac

J 2016

Competitive Upconversion-Linked Immunosorbent Assay for the Sensitive Detection of Diclofenac

HLAVÁČEK, Antonín, Zdeněk FARKA, Maria HUEBNER, Veronika HORŇÁKOVÁ, Daniel NĚMEČEK et. al.

Základní údaje

Originální název

Competitive Upconversion-Linked Immunosorbent Assay for the Sensitive Detection of Diclofenac

Autoři

HLAVÁČEK, Antonín (203 Česká republika, domácí), Zdeněk FARKA (203 Česká republika, domácí), Maria HUEBNER (276 Německo), Veronika HORŇÁKOVÁ (203 Česká republika, domácí), Daniel NĚMEČEK (203 Česká republika, domácí), Reinhard NIESSNER (276 Německo), Petr SKLÁDAL (203 Česká republika, garant, domácí), Dietmar KNOPP (276 Německo) a Hans Heiner GORRIS (276 Německo)

Vydání

Analytical Chemistry, Washington, D.C., USA, American Chemical Society, 2016, 0003-2700

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 6.320

Kód RIV

RIV/00216224:14740/16:00090133

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1021/acs.analchem.6b01083

UT WoS

000377631000065

Klíčová slova anglicky

antibody; bioconjugation; diclofenac; immunoassay; pharmaceutical micropollutant; photon-upconversion

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 15. 9. 2018 18:49, doc. Mgr. Zdeněk Farka, Ph.D.

Anotace

V originále

Photon-upconverting nanoparticles (UCNPs) emit light of shorter wavelength under near-infrared excitation and thus avoid optical background interference. We have exploited this unique photophysical feature to establish a sensitive competitive immunoassay for the detection of the pharmaceutical micropollutant diclofenac (DCF) in water. The so-called upconversion-linked immunosorbent assay (ULISA) was critically dependent on the design of the upconversion luminescent detection label. Silica-coated UCNPs (50 nm in diameter) exposing carboxyl groups on the surface were conjugated to a secondary anti-IgG antibody. We investigated the structure and monodispersity of the nanoconjugates in detail. Using a highly affine anti-DCF primary antibody, the optimized ULISA reached a detection limit of 0.05 ng DCF per mL. This performance came close to a conventional enzyme-linked immunosorbent assay (ELISA) without the need for an enzyme-mediated signal amplification step. The ULISA was further employed for analyzing drinking and surface water samples. The results were consistent with a conventional ELISA as well as liquid chromatography–mass spectrometry (LC–MS).

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

EE2.3.30.0009, projekt VaV

Název: Zaměstnáním čerstvých absolventů doktorského studia k vědecké excelenci

LD15023, projekt VaV

Název: Příprava diskrétních biokonjugátů photon-upconversion nanočástic

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Příprava diskrétních biokonjugátů photon-upconversion nanočástic

Citovat

HLAVÁČEK, Antonín, Zdeněk FARKA, Maria HUEBNER, Veronika HORŇÁKOVÁ, Daniel NĚMEČEK, Reinhard NIESSNER, Petr SKLÁDAL, Dietmar KNOPP a Hans Heiner GORRIS. Competitive Upconversion-Linked Immunosorbent Assay for the Sensitive Detection of Diclofenac. Analytical Chemistry. Washington, D.C., USA: American Chemical Society, 2016, roč. 88, č. 11, s. 6011-6017. ISSN 0003-2700. Dostupné z: https://dx.doi.org/10.1021/acs.analchem.6b01083.

@article{1347122,
   author = {Hlaváček, Antonín and Farka, Zdeněk and Huebner, Maria and Horňáková, Veronika and Němeček, Daniel and Niessner, Reinhard and Skládal, Petr and Knopp, Dietmar and Gorris, Hans Heiner},
   article_location = {Washington, D.C., USA},
   article_number = {11},
   doi = {http://dx.doi.org/10.1021/acs.analchem.6b01083},
   keywords = {antibody; bioconjugation; diclofenac; immunoassay; pharmaceutical micropollutant; photon-upconversion},
   language = {eng},
   issn = {0003-2700},
   journal = {Analytical Chemistry},
   title = {Competitive Upconversion-Linked Immunosorbent Assay for the Sensitive Detection of Diclofenac},
   url = {http://pubs.acs.org/doi/abs/10.1021/acs.analchem.6b01083},
   volume = {88},
   year = {2016}
}

TY  - JOUR
ID  - 1347122
AU  - Hlaváček, Antonín - Farka, Zdeněk - Huebner, Maria - Horňáková, Veronika - Němeček, Daniel - Niessner, Reinhard - Skládal, Petr - Knopp, Dietmar - Gorris, Hans Heiner
PY  - 2016
TI  - Competitive Upconversion-Linked Immunosorbent Assay for the Sensitive Detection of Diclofenac
JF  - Analytical Chemistry
VL  - 88
IS  - 11
SP  - 6011-6017
EP  - 6011-6017
PB  - American Chemical Society
SN  - 00032700
KW  - antibody
KW  - bioconjugation
KW  - diclofenac
KW  - immunoassay
KW  - pharmaceutical micropollutant
KW  - photon-upconversion
UR  - http://pubs.acs.org/doi/abs/10.1021/acs.analchem.6b01083
L2  - http://pubs.acs.org/doi/abs/10.1021/acs.analchem.6b01083
N2  - Photon-upconverting nanoparticles (UCNPs) emit light of shorter wavelength under near-infrared excitation and thus avoid optical background interference. We have exploited this unique photophysical feature to establish a sensitive competitive immunoassay for the detection of the pharmaceutical micropollutant diclofenac (DCF) in water. The so-called upconversion-linked immunosorbent assay (ULISA) was critically dependent on the design of the upconversion luminescent detection label. Silica-coated UCNPs (50 nm in diameter) exposing carboxyl groups on the surface were conjugated to a secondary anti-IgG antibody. We investigated the structure and monodispersity of the nanoconjugates in detail. Using a highly affine anti-DCF primary antibody, the optimized ULISA reached a detection limit of 0.05 ng DCF per mL. This performance came close to a conventional enzyme-linked immunosorbent assay (ELISA) without the need for an enzyme-mediated signal amplification step. The ULISA was further employed for analyzing drinking and surface water samples. The results were consistent with a conventional ELISA as well as liquid chromatography–mass spectrometry (LC–MS).
ER  -

HLAVÁČEK, Antonín, Zdeněk FARKA, Maria HUEBNER, Veronika HORŇÁKOVÁ, Daniel NĚMEČEK, Reinhard NIESSNER, Petr SKLÁDAL, Dietmar KNOPP a Hans Heiner GORRIS. Competitive Upconversion-Linked Immunosorbent Assay for the Sensitive Detection of Diclofenac. \textit{Analytical Chemistry}. Washington, D.C., USA: American Chemical Society, 2016, roč.~88, č.~11, s.~6011-6017. ISSN~0003-2700. Dostupné z: https://dx.doi.org/10.1021/acs.analchem.6b01083.

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