2016
Real-time PCR a digital PCR v diagnostice parazitů v potravinách
RESLOVÁ, Nikol, Martin KAŠNÝ, Michal SLANÝ, Silvia MONTEIRO, Ricardo SANTOS et. al.Základní údaje
Originální název
Real-time PCR a digital PCR v diagnostice parazitů v potravinách
Název česky
Real-time PCR a digital PCR v diagnostice parazitů v potravinách
Název anglicky
Real-time PCR a digital PCR in disgnostics of parasitological agents in food
Autoři
RESLOVÁ, Nikol (203 Česká republika, garant, domácí), Martin KAŠNÝ (203 Česká republika, domácí), Michal SLANÝ (203 Česká republika), Silvia MONTEIRO (620 Portugalsko) a Ricardo SANTOS (620 Portugalsko)
Vydání
XV. Tomáškovy dny mladých mikrobiologů, 2016
Další údaje
Jazyk
čeština
Typ výsledku
Prezentace na konferencích
Obor
10600 1.6 Biological sciences
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Kód RIV
RIV/00216224:14310/16:00090148
Organizační jednotka
Přírodovědecká fakulta
ISBN
978-80-210-8255-7
Klíčová slova česky
real-time PCR; digitální PCR; diagnostika potravinových parazitů; giardia intestinalis; toxoplasma gondii
Klíčová slova anglicky
real-time PCR; digital PCR; diagnostics of foodborne parasites; giardia intestinalis; toxoplasma gondii
Změněno: 25. 3. 2017 08:10, RNDr. Martin Kašný, Ph.D.
V originále
In recent years, the increase of reported outbreaks causing human-diseases associated with food-borne parasitic infections (uni/multi-cellular) arising from meat consumption has been recorded. This trend could be affected e.g. by the changes in farming management towards bio-production, globalization of food market, global climate change and also by the meat processing. This situation is consequently inducing the need for improvement of the diagnostic applications. Our work is focused on development of a reliable comprehensive molecular diagnostic method useful for rapid control of meat products. We adopted high sensitive multiplex oligonucleotide ligation-PCR assay - MOL-PCR, enabling direct and simultaneous detection of multiple nucleic acid signatures of parasites from complex samples and xMAP technology representing a novel detection platform based on magnetic microspheres. Visualization of the products is then realized via qualitative and quantitative MAGPIX instrumentation. Up to this date, the specific molecular probes allowing the detection and capturing of targeted DNA originating from two parasitic worms - Trichinella spiralis (nematode; partial sequence of 18S rRNA gene) and Taenia saginata (cestode; partial sequence of mitochondrial COX1 gene) were designed. Reaction conditions of MOL-PCR were optimized for simultaneous duplex assay. Currently, the calibration of MAGPIX system is in process.
Anglicky
In recent years, the increase of reported outbreaks causing human-diseases associated with food-borne parasitic infections (uni/multi-cellular) arising from meat consumption has been recorded. This trend could be affected e.g. by the changes in farming management towards bio-production, globalization of food market, global climate change and also by the meat processing. This situation is consequently inducing the need for improvement of the diagnostic applications. Our work is focused on development of a reliable comprehensive molecular diagnostic method useful for rapid control of meat products. We adopted high sensitive multiplex oligonucleotide ligation-PCR assay - MOL-PCR, enabling direct and simultaneous detection of multiple nucleic acid signatures of parasites from complex samples and xMAP technology representing a novel detection platform based on magnetic microspheres. Visualization of the products is then realized via qualitative and quantitative MAGPIX instrumentation. Up to this date, the specific molecular probes allowing the detection and capturing of targeted DNA originating from two parasitic worms - Trichinella spiralis (nematode; partial sequence of 18S rRNA gene) and Taenia saginata (cestode; partial sequence of mitochondrial COX1 gene) were designed. Reaction conditions of MOL-PCR were optimized for simultaneous duplex assay. Currently, the calibration of MAGPIX system is in process.
Návaznosti
| MUNI/A/1325/2015, interní kód MU |
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