2016
A modern molecular approach in food safety and food-borne parasitoses
RESLOVÁ, Nikol; Martin KAŠNÝ; Michal SLANÝ a Petr KRÁLÍKZákladní údaje
Originální název
A modern molecular approach in food safety and food-borne parasitoses
Název anglicky
A modern molecular approach in food safety and food-borne parasitoses
Autoři
Vydání
XII. české a slovenské parazitologické dny, 2016
Další údaje
Jazyk
čeština
Typ výsledku
Prezentace na konferencích
Obor
10600 1.6 Biological sciences
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Označené pro přenos do RIV
Ano
Kód RIV
RIV/00216224:14310/16:00090149
Organizační jednotka
Přírodovědecká fakulta
Klíčová slova česky
diagnostika; potravinoví parazité; MOL-PCR; MAGPIX; masové produkty; trichinella spp.; taenia spp.; toxoplasma gondii
Klíčová slova anglicky
diagnostics; foodborne parasites; MOL-PCR; MAGPIX; meat products; trichinella spp.; taenia spp.; toxoplasma gondii
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 14. 3. 2018 09:38, Mgr. Lucie Jarošová, DiS.
V originále
In recent years, there has been an increase in the numbers of reported outbreaks, cases, associated with food-borne parasitic infections arising from meat consumption. This trend is affected by the changes in farming practices towards bio-production, globalization of food market, global climate change and also by the way of meat processing. All these facts facilitate the risks of widespread disseminations of food-borne diseases, including the pathogens of protozoa and multi-cellular parasites such as Toxoplasma gondii, Trichinella spiralis or Taenia saginata, and consequently induce the need of improvement of the various diagnostic tools for food-borne agents in the final food products such as meat. In relation to this issue, our work is focused on development of reliable comprehensive molecular diagnostic method useful for rapid control of meat products on the market. For this purpose, we adopted high sensitive multiplex oligonucleotide ligation- PCR assay - MOL-PCR, enabling direct and simultaneous detection of multiple nucleic acid signatures from complex mixtures potentially containing the DNA originating from number of different parasitic organisms and xMAP technology representing the novel platform based on magnetic microspheres. Visualization of the products is then realized via MAGPIX instrument for qualitative and quantitative readout of signal. Up to this date, the specific molecular probes allowing the detection and capturing of targeted DNA from two parasitic worms - Trichinella spiralis (partial sequence of 18S rRNA gene) and Taenia saginata (partial sequence of mitochondrial COX1 gene) were designed. Reaction conditions of MOL-PCR were optimized for simultaneous duplex assay. Currently, the calibration of MAGPIX system is in process together with arranging procedure for preparing the sample – from election of the most suitable input material to DNA isolation.
Anglicky
In recent years, there has been an increase in the numbers of reported outbreaks, cases, associated with food-borne parasitic infections arising from meat consumption. This trend is affected by the changes in farming practices towards bio-production, globalization of food market, global climate change and also by the way of meat processing. All these facts facilitate the risks of widespread disseminations of food-borne diseases, including the pathogens of protozoa and multi-cellular parasites such as Toxoplasma gondii, Trichinella spiralis or Taenia saginata, and consequently induce the need of improvement of the various diagnostic tools for food-borne agents in the final food products such as meat. In relation to this issue, our work is focused on development of reliable comprehensive molecular diagnostic method useful for rapid control of meat products on the market. For this purpose, we adopted high sensitive multiplex oligonucleotide ligation- PCR assay - MOL-PCR, enabling direct and simultaneous detection of multiple nucleic acid signatures from complex mixtures potentially containing the DNA originating from number of different parasitic organisms and xMAP technology representing the novel platform based on magnetic microspheres. Visualization of the products is then realized via MAGPIX instrument for qualitative and quantitative readout of signal. Up to this date, the specific molecular probes allowing the detection and capturing of targeted DNA from two parasitic worms - Trichinella spiralis (partial sequence of 18S rRNA gene) and Taenia saginata (partial sequence of mitochondrial COX1 gene) were designed. Reaction conditions of MOL-PCR were optimized for simultaneous duplex assay. Currently, the calibration of MAGPIX system is in process together with arranging procedure for preparing the sample – from election of the most suitable input material to DNA isolation.
Návaznosti
| MUNI/A/1325/2015, interní kód MU |
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