Evolution of Telomeres in Schizosaccharomyces pombe and Its
Possible  Relationship to the Diversification of Telomere
Binding Proteins

J 2016

Evolution of Telomeres in Schizosaccharomyces pombe and Its Possible Relationship to the Diversification of Telomere Binding Proteins

SEPSIOVA, Regina; Ivona NEČASOVÁ; Smaranda WILLCOX; Katarina PROCHAZKOVA; Peter GORILÁK et al.

Základní údaje

Originální název

Evolution of Telomeres in Schizosaccharomyces pombe and Its Possible Relationship to the Diversification of Telomere Binding Proteins

Autoři

SEPSIOVA, Regina; Ivona NEČASOVÁ; Smaranda WILLCOX; Katarina PROCHAZKOVA; Peter GORILÁK; Jozef NOSEK; Ctirad HOFR; Jack D. GRIFFITH a Ĺubomír TOMÁŠKA

Vydání

Plos one, San Francisco, Public Library of Science, 2016, 1932-6203

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10610 Biophysics

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Full Text

Impakt faktor

Impact factor: 2.806

Kód RIV

RIV/00216224:14740/16:00088004

Organizační jednotka

Středoevropský technologický institut

DOI

https://doi.org/10.1371/journal.pone.0154225

UT WoS

000374898500167

EID Scopus

2-s2.0-84964624139

Klíčová slova anglicky

MYB-DOMAIN PROTEIN; FISSION YEAST; HUMAN RAP1; USTILAGO-MAYDIS; TAY1 PROTEIN; DNA; TAZ1; TRF2; IDENTIFICATION; SEQUENCE

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 17. 12. 2019 14:09, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Telomeres of nuclear chromosomes are usually composed of an array of tandemly repeated sequences that are recognized by specific Myb domain containing DNA-binding proteins (telomere-binding proteins, TBPs). Whereas in many eukaryotes the length and sequence of the telomeric repeat is relatively conserved, telomeric sequences in various yeasts are highly variable. Schizosaccharomyces pombe provides an excellent model for investigation of co-evolution of telomeres and TBPs. First, telomeric repeats of S. pombe differ from the canonical mammalian type TTAGGG sequence. Second, S. pombe telomeres exhibit a high degree of intratelomeric heterogeneity. Third, S. pombe contains all types of known TBPs (Rap1p [a version unable to bind DNA], Tay1p/Teb1p, and Taz1p) that are employed by various yeast species to protect their telomeres. With the aim of reconstructing evolutionary paths leading to a separation of roles between Teb1p and Taz1p, we performed a comparative analysis of the DNA-binding properties of both proteins using combined qualitative and quantitative biochemical approaches. Visualization of DNA-protein complexes by electron microscopy revealed qualitative differences of binding of Teb1p and Taz1p to mammalian type and fission yeast telomeres. Fluorescence anisotropy analysis quantified the binding affinity of Teb1p and Taz1p to three different DNA substrates. Additionally, we carried out electrophoretic mobility shift assays using mammalian type telomeres and native substrates (telomeric repeats, histone-box sequences) as well as their mutated versions. We observed relative DNA sequence binding flexibility of Taz1p and higher binding stringency of Teb1p when both proteins were compared directly to each other. These properties may have driven replacement of Teb1p by Taz1p as the TBP in fission yeast.

Návaznosti

GAP205/12/0550, projekt VaV

Název: Dynamika vzniku shelterinového komplexu

Investor: Grantová agentura ČR, Dynamika vzniku shelterinového komplexu

LH13054, projekt VaV

Název: Studium dynamiky telomerových proteinových komplexů zajišťujících stabilitu genomu (Akronym: TeloProtGen)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Studium dynamiky telomerových proteinových komplexů zajišťujících stabilitu genomu

Citovat

SEPSIOVA, Regina; Ivona NEČASOVÁ; Smaranda WILLCOX; Katarina PROCHAZKOVA; Peter GORILÁK; Jozef NOSEK; Ctirad HOFR; Jack D. GRIFFITH a Ĺubomír TOMÁŠKA. Evolution of Telomeres in Schizosaccharomyces pombe and Its Possible Relationship to the Diversification of Telomere Binding Proteins. Plos one. San Francisco: Public Library of Science, 2016, roč. 11, č. 4, s. 1-17. ISSN 1932-6203. Dostupné z: https://doi.org/10.1371/journal.pone.0154225.

@article{1348337,
   author = {Sepsiova, Regina and Nečasová, Ivona and Willcox, Smaranda and Prochazkova, Katarina and Gorilák, Peter and Nosek, Jozef and Hofr, Ctirad and Griffith, Jack D. and Tomáška, Ĺubomír},
   article_location = {San Francisco},
   article_number = {4},
   doi = {https://doi.org/10.1371/journal.pone.0154225},
   keywords = {MYB-DOMAIN PROTEIN; FISSION YEAST; HUMAN RAP1; USTILAGO-MAYDIS; TAY1 PROTEIN; DNA; TAZ1; TRF2; IDENTIFICATION; SEQUENCE},
   language = {eng},
   issn = {1932-6203},
   journal = {Plos one},
   title = {Evolution of Telomeres in Schizosaccharomyces pombe and Its Possible Relationship to the Diversification of Telomere Binding Proteins},
   url = {http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0154225},
   volume = {11},
   year = {2016}
}

TY  - JOUR
ID  - 1348337
AU  - Sepsiova, Regina - Nečasová, Ivona - Willcox, Smaranda - Prochazkova, Katarina - Gorilák, Peter - Nosek, Jozef - Hofr, Ctirad - Griffith, Jack D. - Tomáška, Ĺubomír
PY  - 2016
TI  - Evolution of Telomeres in Schizosaccharomyces pombe and Its Possible Relationship to the Diversification of Telomere Binding Proteins
JF  - Plos one
VL  - 11
IS  - 4
SP  - 1-17
EP  - 1-17
PB  - Public Library of Science
SN  - 19326203
KW  - MYB-DOMAIN PROTEIN
KW  - FISSION YEAST
KW  - HUMAN RAP1
KW  - USTILAGO-MAYDIS
KW  - TAY1 PROTEIN
KW  - DNA
KW  - TAZ1
KW  - TRF2
KW  - IDENTIFICATION
KW  - SEQUENCE
UR  - http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0154225
L2  - http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0154225
N2  - Telomeres of nuclear chromosomes are usually composed of an array of tandemly repeated sequences that are recognized by specific Myb domain containing DNA-binding proteins (telomere-binding proteins, TBPs). Whereas in many eukaryotes the length and sequence of the telomeric repeat is relatively conserved, telomeric sequences in various yeasts are highly variable. Schizosaccharomyces pombe provides an excellent model for investigation of co-evolution of telomeres and TBPs. First, telomeric repeats of S. pombe differ from the canonical mammalian type TTAGGG sequence. Second, S. pombe telomeres exhibit a high degree of intratelomeric heterogeneity. Third, S. pombe contains all types of known TBPs (Rap1p [a version unable to bind DNA], Tay1p/Teb1p, and Taz1p) that are employed by various yeast species to protect their telomeres. With the aim of reconstructing evolutionary paths leading to a separation of roles between Teb1p and Taz1p, we performed a comparative analysis of the DNA-binding properties of both proteins using combined qualitative and quantitative biochemical approaches. Visualization of DNA-protein complexes by electron microscopy revealed qualitative differences of binding of Teb1p and Taz1p to mammalian type and fission yeast telomeres. Fluorescence anisotropy analysis quantified the binding affinity of Teb1p and Taz1p to three different DNA substrates. Additionally, we carried out electrophoretic mobility shift assays using mammalian type telomeres and native substrates (telomeric repeats, histone-box sequences) as well as their mutated versions. We observed relative DNA sequence binding flexibility of Taz1p and higher binding stringency of Teb1p when both proteins were compared directly to each other. These properties may have driven replacement of Teb1p by Taz1p as the TBP in fission yeast.
ER  -

SEPSIOVA, Regina; Ivona NEČASOVÁ; Smaranda WILLCOX; Katarina PROCHAZKOVA; Peter GORILÁK; Jozef NOSEK; Ctirad HOFR; Jack D. GRIFFITH a Ĺubomír TOMÁŠKA. Evolution of Telomeres in Schizosaccharomyces pombe and Its Possible Relationship to the Diversification of Telomere Binding Proteins. \textit{Plos one}. San Francisco: Public Library of Science, 2016, roč.~11, č.~4, s.~1-17. ISSN~1932-6203. Dostupné z: https://doi.org/10.1371/journal.pone.0154225.

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