Interactions of fluorescent dye SYBR Green I with natural and
7-deazaguanine-modified DNA studied by fluorescence and
electrochemical methods

J 2016

Interactions of fluorescent dye SYBR Green I with natural and 7-deazaguanine-modified DNA studied by fluorescence and electrochemical methods

DUDOVÁ, Zdenka; Jan ŠPAČEK; Luděk HAVRAN; Hana PIVOŇKOVÁ; Miroslav FOJTA et al.

Základní údaje

Originální název

Interactions of fluorescent dye SYBR Green I with natural and 7-deazaguanine-modified DNA studied by fluorescence and electrochemical methods

Autoři

DUDOVÁ, Zdenka; Jan ŠPAČEK; Luděk HAVRAN; Hana PIVOŇKOVÁ a Miroslav FOJTA

Vydání

Monatshefte fur Chemie, Wien, Springer Wien, 2016, 0026-9247

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10403 Physical chemistry

Stát vydavatele

Rakousko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 1.282

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14740/16:00090268

Organizační jednotka

Středoevropský technologický institut

Klíčová slova anglicky

DNA; 7-Deazaguanine; PCR; Fluorescence; Quenching; DNA interaction; DNA modification; Electrochemical oxidation; Graphite electrode

Štítky

rivok

Změněno: 17. 3. 2017 15:22, Mgr. Eva Špillingová

Anotace

V originále

SYBR Green I (SG) is a fluorescent dye applied in various techniques of DNA analysis, including fluorescent staining of electrophoretic gels, quantitative polymerase chain reaction, etc. SG binds selectively to double-stranded DNA via intercalation and minor groove interactions, resulting in a considerable enhancement of fluorescence of the dye. Modification of DNA by partial or full replacement of natural purine nucleobase guanine (G) with its synthetic analog 7-deazaguanine (G*) or its derivatives was shown to cause the SG fluorescence quenching. In this paper, we present a comparative study of interactions of SG with natural DNA fragments and with DNA fragments modified with G* by means of fluorescence and electrochemical methods. Competition between unmodified (forming strongly fluorescent complex with SG) and fully G*-modified (not contributing significantly to overall fluorescence signal) DNA fragments for the dye was studied via changes in the fluorescence intensity. In addition, association interactions of natural or G*-modified DNA with SG in solution were monitored by adsorptive transfer stripping square wave voltammetry at a pyrolytic graphite electrode using a signal of SG electrooxidation. We show that SG binds both natural and G*-modified DNA with similar apparent affinity and selectivity for the double-stranded DNA. [GRAPHICS] .

Citovat

DUDOVÁ, Zdenka; Jan ŠPAČEK; Luděk HAVRAN; Hana PIVOŇKOVÁ a Miroslav FOJTA. Interactions of fluorescent dye SYBR Green I with natural and 7-deazaguanine-modified DNA studied by fluorescence and electrochemical methods. Monatshefte fur Chemie. Wien: Springer Wien, 2016, roč. 147, č. 1, s. 13-20. ISSN 0026-9247. Dostupné z: https://doi.org/10.1007/s00706-015-1578-5.

@article{1348704,
   author = {Dudová, Zdenka and Špaček, Jan and Havran, Luděk and Pivoňková, Hana and Fojta, Miroslav},
   article_location = {Wien},
   article_number = {1},
   doi = {https://doi.org/10.1007/s00706-015-1578-5},
   keywords = {DNA; 7-Deazaguanine; PCR; Fluorescence; Quenching; DNA interaction; DNA modification; Electrochemical oxidation; Graphite electrode},
   language = {eng},
   issn = {0026-9247},
   journal = {Monatshefte fur Chemie},
   title = {Interactions of fluorescent dye SYBR Green I with natural and 7-deazaguanine-modified DNA studied by fluorescence and electrochemical methods},
   url = {http://link.springer.com/article/10.1007%2Fs00706-015-1578-5},
   volume = {147},
   year = {2016}
}

TY  - JOUR
ID  - 1348704
AU  - Dudová, Zdenka - Špaček, Jan - Havran, Luděk - Pivoňková, Hana - Fojta, Miroslav
PY  - 2016
TI  - Interactions of fluorescent dye SYBR Green I with natural and 7-deazaguanine-modified DNA studied by fluorescence and electrochemical methods
JF  - Monatshefte fur Chemie
VL  - 147
IS  - 1
SP  - 13-20
EP  - 13-20
PB  - Springer Wien
SN  - 00269247
KW  - DNA
KW  - 7-Deazaguanine
KW  - PCR
KW  - Fluorescence
KW  - Quenching
KW  - DNA interaction
KW  - DNA modification
KW  - Electrochemical oxidation
KW  - Graphite electrode
UR  - http://link.springer.com/article/10.1007%2Fs00706-015-1578-5
L2  - http://link.springer.com/article/10.1007%2Fs00706-015-1578-5
N2  - SYBR Green I (SG) is a fluorescent dye applied in various techniques of DNA analysis, including fluorescent staining of electrophoretic gels, quantitative polymerase chain reaction, etc. SG binds selectively to double-stranded DNA via intercalation and minor groove interactions, resulting in a considerable enhancement of fluorescence of the dye. Modification of DNA by partial or full replacement of natural purine nucleobase guanine (G) with its synthetic analog 7-deazaguanine (G*) or its derivatives was shown to cause the SG fluorescence quenching. In this paper, we present a comparative study of interactions of SG with natural DNA fragments and with DNA fragments modified with G* by means of fluorescence and electrochemical methods. Competition between unmodified (forming strongly fluorescent complex with SG) and fully G*-modified (not contributing significantly to overall fluorescence signal) DNA fragments for the dye was studied via changes in the fluorescence intensity. In addition, association interactions of natural or G*-modified DNA with SG in solution were monitored by adsorptive transfer stripping square wave voltammetry at a pyrolytic graphite electrode using a signal of SG electrooxidation. We show that SG binds both natural and G*-modified DNA with similar apparent affinity and selectivity for the double-stranded DNA. [GRAPHICS] .
ER  -

DUDOVÁ, Zdenka; Jan ŠPAČEK; Luděk HAVRAN; Hana PIVOŇKOVÁ a Miroslav FOJTA. Interactions of fluorescent dye SYBR Green I with natural and 7-deazaguanine-modified DNA studied by fluorescence and electrochemical methods. \textit{Monatshefte fur Chemie}. Wien: Springer Wien, 2016, roč.~147, č.~1, s.~13-20. ISSN~0026-9247. Dostupné z: https://doi.org/10.1007/s00706-015-1578-5.

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