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@article{1348705,
author = {Dudová, Zdenka and Pivoňková, Hana and Havran, Luděk and Fojta, Miroslav},
article_location = {USTI NAD LABEM},
keywords = {Sybr Green I; Pyrolytic graphite electrode; Square wave voltammetry; PCR},
language = {cze},
journal = {XXXV MODERNI ELEKTROCHEMICKE METODY},
title = {Electrochemical Analysis of Sybr Green I and its Interaction with DNA},
year = {2015}
}
TY - JOUR
ID - 1348705
AU - Dudová, Zdenka - Pivoňková, Hana - Havran, Luděk - Fojta, Miroslav
PY - 2015
TI - Electrochemical Analysis of Sybr Green I and its Interaction with DNA
JF - XXXV MODERNI ELEKTROCHEMICKE METODY
SP - 35-37
EP - 35-37
PB - LENKA SRSENOVA-BEST SERVIS
KW - Sybr Green I
KW - Pyrolytic graphite electrode
KW - Square wave voltammetry
KW - PCR
N2 - There are used many methods such real-time PCR and electrophoresis in molecular biology and biochemistry which are based on utilization of a fluorescent dye Sybr Green I (SG) for selective detection of double stranded (ds) DNA and its quantification. SG is a planar molecule (see Fig. 1) that intercalates into the DNA double helix and simultaneously can bind into minor groove of dsDNA. In our work we focused on electrochemical behavior of SG on a pyrolytic graphite electrode (PGE) using square-wave voltammetry (SWV). The voltammetric method was used in measurements of SG interactions with DNA at the PGE.
ER -
DUDOVÁ, Zdenka, Hana PIVOŇKOVÁ, Luděk HAVRAN a Miroslav FOJTA. Electrochemical Analysis of Sybr Green I and its Interaction with DNA. \textit{XXXV MODERNI ELEKTROCHEMICKE METODY}. USTI NAD LABEM: LENKA SRSENOVA-BEST SERVIS, 2015, s.~35-37.
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