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@article{1350521,
author = {Týčová, Anna and Prikryl, Jan and Foret, František},
article_location = {HOBOKEN},
article_number = {7-8},
doi = {http://dx.doi.org/10.1002/elps.201500467},
keywords = {Capillary electrophoresis; Grinding; Mass spectrometry; Nanospray tip; 3D printing},
language = {eng},
issn = {0173-0835},
journal = {Electrophoresis},
title = {Reproducible preparation of nanospray tips for capillary electrophoresis coupled to mass spectrometry using 3D printed grinding device},
url = {http://onlinelibrary.wiley.com/doi/10.1002/elps.201500467/abstract;jsessionid=D2A00E0B1FE8AD7CF8D0B92774256257.f03t03},
volume = {37},
year = {2016}
}
TY - JOUR
ID - 1350521
AU - Týčová, Anna - Prikryl, Jan - Foret, František
PY - 2016
TI - Reproducible preparation of nanospray tips for capillary electrophoresis coupled to mass spectrometry using 3D printed grinding device
JF - Electrophoresis
VL - 37
IS - 7-8
SP - 924-930
EP - 924-930
PB - WILEY-BLACKWELL
SN - 01730835
KW - Capillary electrophoresis
KW - Grinding
KW - Mass spectrometry
KW - Nanospray tip
KW - 3D printing
UR - http://onlinelibrary.wiley.com/doi/10.1002/elps.201500467/abstract;jsessionid=D2A00E0B1FE8AD7CF8D0B92774256257.f03t03
L2 - http://onlinelibrary.wiley.com/doi/10.1002/elps.201500467/abstract;jsessionid=D2A00E0B1FE8AD7CF8D0B92774256257.f03t03
N2 - The use of high quality fused silica capillary nanospray tips is critical for obtaining reliable and reproducible electrospray/MS data; however, reproducible laboratory preparation of such tips is a challenging task. In this work, we report on the design and construction of low-cost grinding device assembled from 3D printed and commercially easily available components. Detailed description and characterization of the grinding device is complemented by freely accessible files in stl and skp format allowing easy laboratory replication of the device. The process of sharpening is aimed at achieving maximal symmetricity, surface smoothness and repeatability of the conus shape. Moreover, the presented grinding device brings possibility to fabricate the nanospray tips of desired dimensions regardless of the commercial availability. On several samples of biological nature ( reserpine, rabbit plasma, and the mixture of three aminoacids), performance of fabricated tips is shown on CE coupled to MS analysis. The special interest is paid to the effect of tip sharpness.
ER -
TÝČOVÁ, Anna, Jan PRIKRYL a František FORET. Reproducible preparation of nanospray tips for capillary electrophoresis coupled to mass spectrometry using 3D printed grinding device. \textit{Electrophoresis}. HOBOKEN: WILEY-BLACKWELL, 2016, roč.~37, 7-8, s.~924-930. ISSN~0173-0835. Dostupné z: https://dx.doi.org/10.1002/elps.201500467.
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