Oleyl-hyaluronan micelles loaded with upconverting
nanoparticles for bio-imaging

J 2015

Oleyl-hyaluronan micelles loaded with upconverting nanoparticles for bio-imaging

POSPISILOVA, M; J MRAZEK; V MATUSKA; Sofiane KETTOU; Milada DUSÍKOVÁ et al.

Základní údaje

Originální název

Oleyl-hyaluronan micelles loaded with upconverting nanoparticles for bio-imaging

Autoři

POSPISILOVA, M; J MRAZEK; V MATUSKA; Sofiane KETTOU; Milada DUSÍKOVÁ; V SVOZIL; Kristina NEŠPOROVÁ; G HUERTA-ANGELES; H VAGNEROVA a V VELEBNY

Vydání

Journal of Nanoparticle Research, Dordrecht, Springer Netherlands, 2015, 1388-0764

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.101

Označené pro přenos do RIV

DOI

https://doi.org/10.1007/s11051-015-3186-z

UT WoS

000361882700001

Klíčová slova anglicky

Upconverting nanoparticles; Hyaluronan; Micelle; Luminescence imaging; Biomedicine

Změněno: 6. 8. 2016 13:27, Mgr. Kristina Nešporová, Ph.D.

Anotace

V originále

Hyaluronan (HA) represents an interesting polymer for nanoparticle coating due to its biocompatibility and enhanced cell interaction via CD44 receptor. Here, we describe incorporation of oleate-capped beta-NaYF4:Yb3+, Er3+ nanoparticles (UCNP-OA) into amphiphilic HA by microemulsion method. Resulting structures have a spherical, micelle-like appearance with a hydrodynamic diameter of 180 nm. UCNP-OA-loaded HA micelles show a good stability in PBS buffer and cell culture media. The intensity of green emission of UCNP-OA-loaded HA micelles in water is about five times higher than that of ligand-free UCNP, indicating that amphiphilic HA effectively protects UCNP luminescence from quenching by water molecules. We found that UCNP-OA-loaded HA micelles in concentrations up to 50 mu g mL(-1) increase cell viability of normal human dermal fibroblasts (NHDF), while viability of human breast adenocarcinoma cells MDA-MB-231 is reduced at these concentrations. The utility of UCNP-OA-loaded HA micelles as a bio-imaging probe was demonstrated in vitro by successful labelling of NHDF and MDA-MB-231 cells overexpressing the CD44 receptor.

Citovat

POSPISILOVA, M; J MRAZEK; V MATUSKA; Sofiane KETTOU; Milada DUSÍKOVÁ; V SVOZIL; Kristina NEŠPOROVÁ; G HUERTA-ANGELES; H VAGNEROVA a V VELEBNY. Oleyl-hyaluronan micelles loaded with upconverting nanoparticles for bio-imaging. Journal of Nanoparticle Research. Dordrecht: Springer Netherlands, 2015, roč. 17, č. 9, 11 s. ISSN 1388-0764. Dostupné z: https://doi.org/10.1007/s11051-015-3186-z.

@article{1350940,
   author = {Pospisilova, M and Mrazek, J and Matuska, V and Kettou, Sofiane and Dusíková, Milada and Svozil, V and Nešporová, Kristina and HuertaandAngeles, G and Vagnerova, H and Velebny, V},
   article_location = {Dordrecht},
   article_number = {9},
   doi = {https://doi.org/10.1007/s11051-015-3186-z},
   keywords = {Upconverting nanoparticles; Hyaluronan; Micelle; Luminescence imaging; Biomedicine},
   language = {eng},
   issn = {1388-0764},
   journal = {Journal of Nanoparticle Research},
   title = {Oleyl-hyaluronan micelles loaded with upconverting nanoparticles for bio-imaging},
   url = {http://dx.doi.org/10.1007/s11051-015-3186-z},
   volume = {17},
   year = {2015}
}

TY  - JOUR
ID  - 1350940
AU  - Pospisilova, M - Mrazek, J - Matuska, V - Kettou, Sofiane - Dusíková, Milada - Svozil, V - Nešporová, Kristina - Huerta-Angeles, G - Vagnerova, H - Velebny, V
PY  - 2015
TI  - Oleyl-hyaluronan micelles loaded with upconverting nanoparticles for bio-imaging
JF  - Journal of Nanoparticle Research
VL  - 17
IS  - 9
PB  - Springer Netherlands
SN  - 13880764
KW  - Upconverting nanoparticles
KW  - Hyaluronan
KW  - Micelle
KW  - Luminescence imaging
KW  - Biomedicine
UR  - http://dx.doi.org/10.1007/s11051-015-3186-z
L2  - http://dx.doi.org/10.1007/s11051-015-3186-z
N2  - Hyaluronan (HA) represents an interesting polymer for nanoparticle coating due to its biocompatibility and enhanced cell interaction via CD44 receptor. Here, we describe incorporation of oleate-capped beta-NaYF4:Yb3+, Er3+ nanoparticles (UCNP-OA) into amphiphilic HA by microemulsion method. Resulting structures have a spherical, micelle-like appearance with a hydrodynamic diameter of 180 nm. UCNP-OA-loaded HA micelles show a good stability in PBS buffer and cell culture media. The intensity of green emission of UCNP-OA-loaded HA micelles in water is about five times higher than that of ligand-free UCNP, indicating that amphiphilic HA effectively protects UCNP luminescence from quenching by water molecules. We found that UCNP-OA-loaded HA micelles in concentrations up to 50 mu g mL(-1) increase cell viability of normal human dermal fibroblasts (NHDF), while viability of human breast adenocarcinoma cells MDA-MB-231 is reduced at these concentrations. The utility of UCNP-OA-loaded HA micelles as a bio-imaging probe was demonstrated in vitro by successful labelling of NHDF and MDA-MB-231 cells overexpressing the CD44 receptor.
ER  -

POSPISILOVA, M; J MRAZEK; V MATUSKA; Sofiane KETTOU; Milada DUSÍKOVÁ; V SVOZIL; Kristina NEŠPOROVÁ; G HUERTA-ANGELES; H VAGNEROVA a V VELEBNY. Oleyl-hyaluronan micelles loaded with upconverting nanoparticles for bio-imaging. \textit{Journal of Nanoparticle Research}. Dordrecht: Springer Netherlands, 2015, roč.~17, č.~9, 11 s. ISSN~1388-0764. Dostupné z: https://doi.org/10.1007/s11051-015-3186-z.

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