Rapid Immunosensing of Salmonella Typhimurium Using
Electrochemical Impedance Spectroscopy: the Effect of Sample
Treatment

J 2016

Rapid Immunosensing of Salmonella Typhimurium Using Electrochemical Impedance Spectroscopy: the Effect of Sample Treatment

FARKA, Zdeněk, Tomáš JUŘÍK, Matěj PASTUCHA, David KOVÁŘ, Karel LACINA et. al.

Základní údaje

Originální název

Rapid Immunosensing of Salmonella Typhimurium Using Electrochemical Impedance Spectroscopy: the Effect of Sample Treatment

Autoři

FARKA, Zdeněk (203 Česká republika, domácí), Tomáš JUŘÍK (203 Česká republika, domácí), Matěj PASTUCHA (203 Česká republika, domácí), David KOVÁŘ (203 Česká republika, domácí), Karel LACINA (203 Česká republika, domácí) a Petr SKLÁDAL (203 Česká republika, garant, domácí)

Vydání

Electroanalysis, Weinheim, Wiley, 2016, 1040-0397

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.851

Kód RIV

RIV/00216224:14740/16:00090535

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1002/elan.201600093

UT WoS

000382579300014

Klíčová slova anglicky

Atomic force microscopy; Biosensor; Immunosensor; Label-free detection; Salmonella Typhimurium

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 28. 9. 2017 11:01, doc. Mgr. Zdeněk Farka, Ph.D.

Anotace

V originále

A label-free immunosensor for rapid detection of Salmonella Typhimurium based on electrochemical impedance spectroscopy was developed. Specific antibody was immobilized to a screen-printed electrode via cysteamine monolayer activated with glutaraldehyde and the impedance was measured between two gold electrodes. Different procedures for sample treatment (combinations of heat and sonication) were tested and their impact on the assay performance was compared. Atomic force microscopy was used to study the effect of the treatment on the cell shape and to confirm the specific binding of Salmonella to the sensing surface. The immunosensor allowed detection of 1×10^3 CFU·mL-1 in 20 min with negligible interference from other bacteria. Wide linear response was obtained in the range between 10^3 CFU·mL-1 and 10^8 CFU·mL-1. The successful detection of Salmonella in spiked milk demonstrates the suitability of sensor for the analysis of real samples.

Návaznosti

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

FARKA, Zdeněk, Tomáš JUŘÍK, Matěj PASTUCHA, David KOVÁŘ, Karel LACINA a Petr SKLÁDAL. Rapid Immunosensing of Salmonella Typhimurium Using Electrochemical Impedance Spectroscopy: the Effect of Sample Treatment. Electroanalysis. Weinheim: Wiley, 2016, roč. 28, č. 8, s. 1803-1809. ISSN 1040-0397. Dostupné z: https://dx.doi.org/10.1002/elan.201600093.

@article{1351272,
   author = {Farka, Zdeněk and Juřík, Tomáš and Pastucha, Matěj and Kovář, David and Lacina, Karel and Skládal, Petr},
   article_location = {Weinheim},
   article_number = {8},
   doi = {http://dx.doi.org/10.1002/elan.201600093},
   keywords = {Atomic force microscopy; Biosensor; Immunosensor; Label-free detection; Salmonella Typhimurium},
   language = {eng},
   issn = {1040-0397},
   journal = {Electroanalysis},
   title = {Rapid Immunosensing of Salmonella Typhimurium Using Electrochemical Impedance Spectroscopy: the Effect of Sample Treatment},
   url = {http://onlinelibrary.wiley.com/doi/10.1002/elan.201600093/full},
   volume = {28},
   year = {2016}
}

TY  - JOUR
ID  - 1351272
AU  - Farka, Zdeněk - Juřík, Tomáš - Pastucha, Matěj - Kovář, David - Lacina, Karel - Skládal, Petr
PY  - 2016
TI  - Rapid Immunosensing of Salmonella Typhimurium Using Electrochemical Impedance Spectroscopy: the Effect of Sample Treatment
JF  - Electroanalysis
VL  - 28
IS  - 8
SP  - 1803-1809
EP  - 1803-1809
PB  - Wiley
SN  - 10400397
KW  - Atomic force microscopy
KW  - Biosensor
KW  - Immunosensor
KW  - Label-free detection
KW  - Salmonella Typhimurium
UR  - http://onlinelibrary.wiley.com/doi/10.1002/elan.201600093/full
L2  - http://onlinelibrary.wiley.com/doi/10.1002/elan.201600093/full
N2  - A label-free immunosensor for rapid detection of Salmonella Typhimurium based on electrochemical impedance spectroscopy was developed. Specific antibody was immobilized to a screen-printed electrode via cysteamine monolayer activated with glutaraldehyde and the impedance was measured between two gold electrodes. Different procedures for sample treatment (combinations of heat and sonication) were tested and their impact on the assay performance was compared. Atomic force microscopy was used to study the effect of the treatment on the cell shape and to confirm the specific binding of Salmonella to the sensing surface. The immunosensor allowed detection of 1×10^3 CFU·mL-1 in 20 min with negligible interference from other bacteria. Wide linear response was obtained in the range between 10^3 CFU·mL-1 and 10^8 CFU·mL-1. The successful detection of Salmonella in spiked milk demonstrates the suitability of sensor for the analysis of real samples.
ER  -

FARKA, Zdeněk, Tomáš JUŘÍK, Matěj PASTUCHA, David KOVÁŘ, Karel LACINA a Petr SKLÁDAL. Rapid Immunosensing of Salmonella Typhimurium Using Electrochemical Impedance Spectroscopy: the Effect of Sample Treatment. \textit{Electroanalysis}. Weinheim: Wiley, 2016, roč.~28, č.~8, s.~1803-1809. ISSN~1040-0397. Dostupné z: https://dx.doi.org/10.1002/elan.201600093.

Podrobný výpis o publikaci