Clinical Potential of Effective Noninvasive Exclusion of
KEL1-Positive Fetuses in KEL1-Negative Pregnant Women

J 2016

Clinical Potential of Effective Noninvasive Exclusion of KEL1-Positive Fetuses in KEL1-Negative Pregnant Women

BÖHMOVA, Jana; Radek VODICKA; Marek LUBUSKY; Iva HOLUSKOVA; Martina STUDNICKOVA et al.

Základní údaje

Originální název

Clinical Potential of Effective Noninvasive Exclusion of KEL1-Positive Fetuses in KEL1-Negative Pregnant Women

Autoři

Vydání

Fetal Diagnosis and Therapy, Basel, Karger, 2016, 1015-3837

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30214 Obstetrics and gynaecology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.699

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/16:00090556

Organizační jednotka

Lékařská fakulta

DOI

https://doi.org/10.1159/000441296

UT WoS

000380144600007

EID Scopus

2-s2.0-84945152345

Klíčová slova anglicky

Noninvasive fetal genotyping; KEL1; Minisequencing; Kell blood group system; Red blood cell alloimmunization; Hemolytic disease of the fetus and newborn

Štítky

EL OK

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 25. 8. 2016 08:52, Ing. Mgr. Věra Pospíšilíková

Anotace

V originále

Background: The clinical importance of assessing the fetal KEL genotype is to exclude 'K'-positive fetuses (genotype KEL1/KEL2) in 'K'-alloimmunized pregnant women (genotype KEL2/KEL2). Noninvasive assessment of the fetal KEL genotype is not yet available in the Czech Republic. Objective: The aim of this study was to assess the fetal KEL1/KEL2 genotype from cell-free fetal DNA in the plasma of KEL2/KEL2 pregnant women. Methods: The fetal genotype was assessed by minisequencing (a dilution series including control samples). A total of 138 pregnant women (between the 8th and 23rd gestational week) were tested by minisequencing. The fetal genotype was further verified by analysis of a buccal swab from the newborn. Results: Minisequencing proved to be a reliable method. In 2.2% (3/138) of the examined women, plasma sample testing failed; 94.8% (128/135) had the KEL2/KEL2 genotype, and a total of 3.1% of fetuses (4/128) had the KEL1/KEL2 genotype. Sensitivity and specificity reached 100% (p < 0.0001). Conclusion: Minisequencing is a reliable method for the assessment of the fetal KEL1 allele from the plasma of KEL2/KEL2 pregnant women.

Citovat

BÖHMOVA, Jana; Radek VODICKA; Marek LUBUSKY; Iva HOLUSKOVA; Martina STUDNICKOVA; Romana KRATOCHVILOVA; Eva KREJCIRIKOVA; Maria JANIKOVA; Veronika DURDOVÁ; Tereza DOLEZALOVÁ; Hana FILIPOVÁ; Ladislav DUŠEK; Ishraq DHAIFALAH; Katherine VOMACKOVA; Marian KACEROVSKY; Martin PROCHAZKA a Radek VRTEL. Clinical Potential of Effective Noninvasive Exclusion of KEL1-Positive Fetuses in KEL1-Negative Pregnant Women. Fetal Diagnosis and Therapy. Basel: Karger, 2016, roč. 40, č. 1, s. 48-53. ISSN 1015-3837. Dostupné z: https://doi.org/10.1159/000441296.

@article{1351373,
   author = {Böhmova, Jana and Vodicka, Radek and Lubusky, Marek and Holuskova, Iva and Studnickova, Martina and Kratochvilova, Romana and Krejcirikova, Eva and Janikova, Maria and Durdová, Veronika and Dolezalová, Tereza and Filipová, Hana and Dušek, Ladislav and Dhaifalah, Ishraq and Vomackova, Katherine and Kacerovsky, Marian and Prochazka, Martin and Vrtel, Radek},
   article_location = {Basel},
   article_number = {1},
   doi = {https://doi.org/10.1159/000441296},
   keywords = {Noninvasive fetal genotyping; KEL1; Minisequencing; Kell blood group system; Red blood cell alloimmunization; Hemolytic disease of the fetus and newborn},
   language = {eng},
   issn = {1015-3837},
   journal = {Fetal Diagnosis and Therapy},
   title = {Clinical Potential of Effective Noninvasive Exclusion of KEL1-Positive Fetuses in KEL1-Negative Pregnant Women},
   volume = {40},
   year = {2016}
}

TY  - JOUR
ID  - 1351373
AU  - Böhmova, Jana - Vodicka, Radek - Lubusky, Marek - Holuskova, Iva - Studnickova, Martina - Kratochvilova, Romana - Krejcirikova, Eva - Janikova, Maria - Durdová, Veronika - Dolezalová, Tereza - Filipová, Hana - Dušek, Ladislav - Dhaifalah, Ishraq - Vomackova, Katherine - Kacerovsky, Marian - Prochazka, Martin - Vrtel, Radek
PY  - 2016
TI  - Clinical Potential of Effective Noninvasive Exclusion of KEL1-Positive Fetuses in KEL1-Negative Pregnant Women
JF  - Fetal Diagnosis and Therapy
VL  - 40
IS  - 1
SP  - 48-53
EP  - 48-53
PB  - Karger
SN  - 10153837
KW  - Noninvasive fetal genotyping
KW  - KEL1
KW  - Minisequencing
KW  - Kell blood group system
KW  - Red blood cell alloimmunization
KW  - Hemolytic disease of the fetus and newborn
N2  - Background: The clinical importance of assessing the fetal KEL genotype is to exclude 'K'-positive fetuses (genotype KEL1/KEL2) in 'K'-alloimmunized pregnant women (genotype KEL2/KEL2). Noninvasive assessment of the fetal KEL genotype is not yet available in the Czech Republic. Objective: The aim of this study was to assess the fetal KEL1/KEL2 genotype from cell-free fetal DNA in the plasma of KEL2/KEL2 pregnant women. Methods: The fetal genotype was assessed by minisequencing (a dilution series including control samples). A total of 138 pregnant women (between the 8th and 23rd gestational week) were tested by minisequencing. The fetal genotype was further verified by analysis of a buccal swab from the newborn. Results: Minisequencing proved to be a reliable method. In 2.2% (3/138) of the examined women, plasma sample testing failed; 94.8% (128/135) had the KEL2/KEL2 genotype, and a total of 3.1% of fetuses (4/128) had the KEL1/KEL2 genotype. Sensitivity and specificity reached 100% (p < 0.0001). Conclusion: Minisequencing is a reliable method for the assessment of the fetal KEL1 allele from the plasma of KEL2/KEL2 pregnant women.
ER  -

BÖHMOVA, Jana; Radek VODICKA; Marek LUBUSKY; Iva HOLUSKOVA; Martina STUDNICKOVA; Romana KRATOCHVILOVA; Eva KREJCIRIKOVA; Maria JANIKOVA; Veronika DURDOVÁ; Tereza DOLEZALOVÁ; Hana FILIPOVÁ; Ladislav DUŠEK; Ishraq DHAIFALAH; Katherine VOMACKOVA; Marian KACEROVSKY; Martin PROCHAZKA a Radek VRTEL. Clinical Potential of Effective Noninvasive Exclusion of KEL1-Positive Fetuses in KEL1-Negative Pregnant Women. \textit{Fetal Diagnosis and Therapy}. Basel: Karger, 2016, roč.~40, č.~1, s.~48-53. ISSN~1015-3837. Dostupné z: https://doi.org/10.1159/000441296.

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