| KOVÁČIK, Jozef, Bořivoj KLEJDUS, Petr BABULA a Josef HEDBAVNY. Age affects not only metabolome but also metal toxicity in Scenedesmus quadricauda cultures. Journal of Hazardous Materials. Amsterdam: Elsevier Science BV., 2016, roč. 306, "APR 5 2016", s. 58-66. ISSN 0304-3894. Dostupné z: https://dx.doi.org/10.1016/j.jhazmat.2015.11.056. |
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@article{1352667,
author = {Kováčik, Jozef and Klejdus, Bořivoj and Babula, Petr and Hedbavny, Josef},
article_location = {Amsterdam},
article_number = {"APR 5 2016"},
doi = {http://dx.doi.org/10.1016/j.jhazmat.2015.11.056},
keywords = {Aging; Antioxidants; Confocal microscopy; DART-Orbitrap MS; Oxidative stress},
language = {eng},
issn = {0304-3894},
journal = {Journal of Hazardous Materials},
title = {Age affects not only metabolome but also metal toxicity in Scenedesmus quadricauda cultures},
volume = {306},
year = {2016}
}
TY - JOUR
ID - 1352667
AU - Kováčik, Jozef - Klejdus, Bořivoj - Babula, Petr - Hedbavny, Josef
PY - 2016
TI - Age affects not only metabolome but also metal toxicity in Scenedesmus quadricauda cultures
JF - Journal of Hazardous Materials
VL - 306
IS - "APR 5 2016"
SP - 58-66
EP - 58-66
PB - Elsevier Science BV.
SN - 03043894
KW - Aging
KW - Antioxidants
KW - Confocal microscopy
KW - DART-Orbitrap MS
KW - Oxidative stress
N2 - Responses of Scenedesmus quadricauda grown in vitro and differing in age (old culture-13 months, young culture-1 month) to short-term cadmium (Cd) or nickel (Ni) excess (24 h) were compared. Higher age of the culture led to lower amount of chlorophylls, ascorbic acid and glutathione but higher signal of ROS. Surprisingly, sucrose was detected using DART-Orbitrap MS in both old and young culture and subsequent quantification confirmed its higher amount (ca. 3-times) in the old culture. Cd affected viability and ROS amount more negatively than Ni that could arise from excessive Cd uptake which was also higher in all treatments than in respective Ni counterparts. Surprisingly, nitric oxide was not extensively different in response to age or metals. Strong induction of phytochelatin 2 is certainly Cd-specific response while Ni also elevated ascorbate content. Krebs cycle acids were more accumulated in the young culture but they were rather elevated in the old culture (citric acid under Ni excess). We conclude that organic solid 'Milieu Bristol' medium we tested is suitable for long-term storage of unicellular green algae (also successfully tested for Coccomyxa sp. and Parachlorella sp.) and the impact of age on metal uptake may be useful for bioremediation purposes.
ER -
KOVÁČIK, Jozef, Bořivoj KLEJDUS, Petr BABULA a Josef HEDBAVNY. Age affects not only metabolome but also metal toxicity in Scenedesmus quadricauda cultures. \textit{Journal of Hazardous Materials}. Amsterdam: Elsevier Science BV., 2016, roč.~306, ''APR 5 2016'', s.~58-66. ISSN~0304-3894. Dostupné z: https://dx.doi.org/10.1016/j.jhazmat.2015.11.056.
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