Concurrent infection of porcine monocyte-derived macrophages
with PRRSV and Haemophilus parasuis

a 2015

Concurrent infection of porcine monocyte-derived macrophages with PRRSV and Haemophilus parasuis

KAVANOVÁ, Lenka; Kateřina NEDBALCOVÁ; Lenka LEVÁ; Martin FALDYNA; Jiří SALÁT et al.

Základní údaje

Originální název

Concurrent infection of porcine monocyte-derived macrophages with PRRSV and Haemophilus parasuis

Název česky

Souběžná infekce prasečích makrofágů derivovaných z monocytů virem PRRSV a Haemophilem parasuis

Autoři

KAVANOVÁ, Lenka; Kateřina NEDBALCOVÁ; Lenka LEVÁ; Martin FALDYNA a Jiří SALÁT

Vydání

5th European veterinary immunology workshop, Vienna, 2015

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

30102 Immunology

Stát vydavatele

Rakousko

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova česky

PRRSV; Haemophilus parasuis; makrofágy derivované z monocytů

Klíčová slova anglicky

PRRSV; Haemophilus parasuis; monocyte-derived macrophages

Příznaky

Mezinárodní význam

Změněno: 31. 8. 2016 10:47, Mgr. Lenka Kavanová, Ph.D.

Anotace

V originále

Porcine reproductive and respiratory syndrome (PRRS) is an important swine disease causing severe economic losses worldwide and can predispose pigs to infection by bacteria such as Haemophilus parasuis. This study was aimed on immune response of porcine monocyte-derived macrophages (MDMF) to simultaneous infection with PRRSV and H. parasuis in vitro. Expression of immunologically-relevant molecules (measured by qRT-PCR) and antimicrobial characteristics such as respiratory burst (measured by luminometry) were tested. MDMFs were sensitive to PRRSV infection and its mortality after 24 hours with PRRSV was high (85%). Concurrent infection with PRRSV and H. parasuis induced lower expression of mRNA for pro-inflammatory cytokines IL-1beta and IL-8 when compared to control. Anti-inflammatory effect of dual infection was also observed on higher expression of mRNA for IL-10. On the contrary, simultaneous infection of MDMF with PRRSV and H. parasuis induced higher expression of mRNA for pro-inflammatory cytokine TNF-alpha. Increased production of reactive oxygen species (ROS) was detected in MDMFs infected with PRRSV in comparison with non-infected MDMFs. On the other hand, simultaneously infected MDMFs decreased production of ROS. Obtained data showed that concurrent infection of MDMFs with PRRSV and H. parasuis elicits anti-inflammatory immune response. PRRSV infection seems to be responsible for elevated levels of ROS which might be one of the causes of high mortality of MDMFs. The work was supported by the Ministry of Education, Youth and Sports of the Czech Republic (project LO1218 under NPU-I program) and the Ministry of Agriculture of the Czech Republic (QJ1210120).

Návaznosti

LO1218, projekt VaV

Název: Zdravé zvíře jako zdroj zdravé potraviny

QJ1210120, projekt VaV

Název: Program zdravotní kontroly ekonomicky významných infekcí v chovech prasat

Citovat

KAVANOVÁ, Lenka; Kateřina NEDBALCOVÁ; Lenka LEVÁ; Martin FALDYNA a Jiří SALÁT. Concurrent infection of porcine monocyte-derived macrophages with PRRSV and Haemophilus parasuis. In 5th European veterinary immunology workshop, Vienna. 2015.

@proceedings{1352774,
   author = {Kavanová, Lenka and Nedbalcová, Kateřina and Levá, Lenka and Faldyna, Martin and Salát, Jiří},
   booktitle = {5th European veterinary immunology workshop, Vienna},
   keywords = {PRRSV; Haemophilus parasuis; monocyte-derived macrophages},
   language = {eng},
   title = {Concurrent infection of porcine monocyte-derived macrophages with PRRSV and Haemophilus parasuis},
   year = {2015}
}

TY  - CONF
ID  - 1352774
AU  - Kavanová, Lenka - Nedbalcová, Kateřina - Levá, Lenka - Faldyna, Martin - Salát, Jiří
PY  - 2015
TI  - Concurrent infection of porcine monocyte-derived macrophages with PRRSV and Haemophilus parasuis
KW  - PRRSV
KW  - Haemophilus parasuis
KW  - monocyte-derived macrophages
N2  - Porcine reproductive and respiratory syndrome (PRRS) is an important swine disease causing severe economic losses worldwide and can predispose pigs to infection by bacteria such as Haemophilus parasuis. This study was aimed on immune response of porcine monocyte-derived macrophages (MDMF) to simultaneous infection with PRRSV and H. parasuis in vitro. Expression of immunologically-relevant molecules (measured by qRT-PCR) and antimicrobial characteristics such as respiratory burst (measured by luminometry) were tested. MDMFs were sensitive to PRRSV infection and its mortality after 24 hours with PRRSV was high (85%). Concurrent infection with PRRSV and H. parasuis induced lower expression of mRNA for pro-inflammatory cytokines IL-1beta and IL-8 when compared to control. Anti-inflammatory effect of dual infection was also observed on higher expression of mRNA for IL-10. On the contrary, simultaneous infection of MDMF with PRRSV and H. parasuis induced higher expression of mRNA for pro-inflammatory cytokine TNF-alpha. Increased production of reactive oxygen species (ROS) was detected in MDMFs infected with PRRSV in comparison with non-infected MDMFs. On the other hand, simultaneously infected MDMFs decreased production of ROS. Obtained data showed that concurrent infection of MDMFs with PRRSV and H. parasuis elicits anti-inflammatory immune response. PRRSV infection seems to be responsible for elevated levels of ROS which might be one of the causes of high mortality of MDMFs. The work was supported by the Ministry of Education, Youth and Sports of the Czech Republic (project LO1218 under NPU-I program) and the Ministry of Agriculture of the Czech Republic (QJ1210120).
ER  -

KAVANOVÁ, Lenka; Kateřina NEDBALCOVÁ; Lenka LEVÁ; Martin FALDYNA a Jiří SALÁT. Concurrent infection of porcine monocyte-derived macrophages with PRRSV and Haemophilus parasuis. In \textit{5th European veterinary immunology workshop, Vienna}. 2015.

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