| RIEDL, J, R POHL, NP ERNSTING, Petr ORSÁG, Miroslav FOJTA a Michal HOCEK. Labelling of nucleosides and oligonucleotides by solvatochromic 4-aminophthalimide fluorophore for studying DNA-protein interactions. CHEMICAL SCIENCE. Cambridge: ROYAL SOC CHEMISTRY, 2012, roč. 3, č. 9, s. 2797-2806. ISSN 2041-6520. Dostupné z: https://dx.doi.org/10.1039/c2sc20404e. |
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@article{1353378,
author = {Riedl, J and Pohl, R and Ernsting, NP and Orság, Petr and Fojta, Miroslav and Hocek, Michal},
article_location = {Cambridge},
article_number = {9},
doi = {http://dx.doi.org/10.1039/c2sc20404e},
language = {eng},
issn = {2041-6520},
journal = {CHEMICAL SCIENCE},
title = {Labelling of nucleosides and oligonucleotides by solvatochromic 4-aminophthalimide fluorophore for studying DNA-protein interactions},
volume = {3},
year = {2012}
}
TY - JOUR
ID - 1353378
AU - Riedl, J - Pohl, R - Ernsting, NP - Orság, Petr - Fojta, Miroslav - Hocek, Michal
PY - 2012
TI - Labelling of nucleosides and oligonucleotides by solvatochromic 4-aminophthalimide fluorophore for studying DNA-protein interactions
JF - CHEMICAL SCIENCE
VL - 3
IS - 9
SP - 2797-2806
EP - 2797-2806
PB - ROYAL SOC CHEMISTRY
SN - 20416520
N2 - Solvatochromic fluorescent 4-aminophthalimide (API) and 4-(N, N-dimethylamino) phthalimide (DAPI) were attached covalently to 2'-deoxycytidine or -adenosine via a non-conjugated propargyl linker by Sonogashira cross-coupling reactions of N-propargylphthalimides with halogenated nucleosides. The nucleosides were phosphorylated to triphosphates and enzymatically incorporated into oligonucleotides by DNA polymerases. API-labelled DNA was used for the detection of DNA protein interactions with either the sequence-specific p53 protein or a non-specific single strand binding (SSB) protein. Both proteins changed the polarity around the fluorophore and increased (2-3 fold) the intensity of API fluorescence.
ER -
RIEDL, J, R POHL, NP ERNSTING, Petr ORSÁG, Miroslav FOJTA a Michal HOCEK. Labelling of nucleosides and oligonucleotides by solvatochromic 4-aminophthalimide fluorophore for studying DNA-protein interactions. \textit{CHEMICAL SCIENCE}. Cambridge: ROYAL SOC CHEMISTRY, 2012, roč.~3, č.~9, s.~2797-2806. ISSN~2041-6520. Dostupné z: https://dx.doi.org/10.1039/c2sc20404e.
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