The identification of Borrelia burgdorferi genospecies isolated
from Ixodes ricinus ticks in the South Moravian region of the
Czech Republic

a 2016

The identification of Borrelia burgdorferi genospecies isolated from Ixodes ricinus ticks in the South Moravian region of the Czech Republic

BONCZEK, Ondřej; Omar ŠERÝ a Alena ŽÁKOVSKÁ

Základní údaje

Originální název

The identification of Borrelia burgdorferi genospecies isolated from Ixodes ricinus ticks in the South Moravian region of the Czech Republic

Autoři

BONCZEK, Ondřej; Omar ŠERÝ a Alena ŽÁKOVSKÁ

Vydání

27. Kongres Československé společnosti mikrobiologické, 2016

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/16:00090765

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-270-0136-1

Klíčová slova anglicky

Borrelia burgdorferi s. l.; Borrelia spielmanii; Ixodes ricinus; genotyping; RealTime PCR; DNA sequencing

Změněno: 9. 9. 2016 12:42, RNDr. Ing. Ondřej Bonczek, Ph.D.

Anotace

V originále

The Ixodes ricinus tick is the most commonly mentioned transmitter of spirochetes belonging to the Borrelia burgdorferi sensu lato (Bbsl) group. The Bbsl complex currently contains at least 18 genospecies associated with particular reservoir hosts, 8 of which are distributed throughout Europe. The aim of this study was to determine the infestation of Bbsl in ticks Ixodes ricinus and the identification of genospecies of Bbsl group by DNA sequencing. During 2008–2015, ticks Ixodes ricinus removed from humans were collected and tested for the presence of Bbsl. DNA isolation from homogenates was performed by UltraClean BloodSpin DNA kit (MoBio) and by automated instrument Prepito (Perkin-Elmer). RealTime PCR assay based on the specific flagellin sequence amplification for the detection of B. burgdorferi s.l. was performed by EliGene Borrelia UNI kit (Elisabeth Pharmacon, Czech Republic). The kit is used in clinical laboratories in the European Union as an in vitro diagnostic medical device. Internal control was used according to the instruction manual of the kit during isolation of DNA and PCR assays to control process of amplification, and to avoid PCR inhibited samples and false negative results. RealTime positive samples were subsequently DNA sequenced. The annual positivity of the removed ticks ranged from 16 – 33%. The most frequently occurring genospecies was B. afzelii, followed by B. garinii, B. valaisiana, B. spielmanii, and finally, B. burgdorferi sensu stricto. The most important finding is the first identification of B. spielmanii in this region [1]. 1. Bonczek O, Žákovská A, Vargová L, Šerý O. Identification of Borrelia burgdorferi genospecies isolated from Ixodes ricinus ticks in the South Moravian region of the Czech Republic. Ann Agric Environ Med. 2015; 22(4): 642–646.

Citovat

BONCZEK, Ondřej; Omar ŠERÝ a Alena ŽÁKOVSKÁ. The identification of Borrelia burgdorferi genospecies isolated from Ixodes ricinus ticks in the South Moravian region of the Czech Republic. In 27. Kongres Československé společnosti mikrobiologické. 2016. ISBN 978-80-270-0136-1.

@proceedings{1353521,
   author = {Bonczek, Ondřej and Šerý, Omar and Žákovská, Alena},
   booktitle = {27. Kongres Československé společnosti mikrobiologické},
   keywords = {Borrelia burgdorferi s. l.; Borrelia spielmanii; Ixodes ricinus; genotyping; RealTime PCR; DNA sequencing},
   language = {eng},
   isbn = {978-80-270-0136-1},
   title = {The identification of Borrelia burgdorferi genospecies isolated from Ixodes ricinus ticks in the South Moravian region of the Czech Republic},
   year = {2016}
}

TY  - CONF
ID  - 1353521
AU  - Bonczek, Ondřej - Šerý, Omar - Žákovská, Alena
PY  - 2016
TI  - The identification of Borrelia burgdorferi genospecies isolated from Ixodes ricinus ticks in the South Moravian region of the Czech Republic
SN  - 9788027001361
KW  - Borrelia burgdorferi s. l.
KW  - Borrelia spielmanii
KW  - Ixodes ricinus
KW  - genotyping
KW  - RealTime PCR
KW  - DNA sequencing
N2  - The Ixodes ricinus tick is the most commonly mentioned transmitter of spirochetes belonging to the Borrelia burgdorferi sensu lato (Bbsl) group. The Bbsl complex currently contains at least 18 genospecies associated with particular reservoir hosts, 8 of which are distributed throughout Europe. The aim of this study was to determine the infestation of Bbsl in ticks Ixodes ricinus and the identification of genospecies of Bbsl group by DNA sequencing. During 2008–2015, ticks Ixodes ricinus removed from humans were collected and tested for the presence of Bbsl. DNA isolation from homogenates was performed by UltraClean BloodSpin DNA kit (MoBio) and by automated instrument Prepito (Perkin-Elmer). RealTime PCR assay based on the specific flagellin sequence amplification for the detection of B. burgdorferi s.l. was performed by EliGene Borrelia UNI kit (Elisabeth Pharmacon, Czech Republic). The kit is used in clinical laboratories in the European Union as an in vitro diagnostic medical device. Internal control was used according to the instruction manual of the kit during isolation of DNA and PCR assays to control process of amplification, and to avoid PCR inhibited samples and false negative results. RealTime positive samples were subsequently DNA sequenced. The annual positivity of the removed ticks ranged from 16 – 33%. The most frequently occurring genospecies was B. afzelii, followed by B. garinii, B. valaisiana, B. spielmanii, and finally, B. burgdorferi sensu stricto. The most important finding is the first identification of B. spielmanii in this region [1]. 1. Bonczek O, Žákovská A, Vargová L, Šerý O. Identification of Borrelia burgdorferi genospecies isolated from Ixodes ricinus ticks in the South Moravian region of the Czech Republic. Ann Agric Environ Med. 2015; 22(4): 642–646.
ER  -

BONCZEK, Ondřej; Omar ŠERÝ a Alena ŽÁKOVSKÁ. The identification of Borrelia burgdorferi genospecies isolated from Ixodes ricinus ticks in the South Moravian region of the Czech Republic. In \textit{27. Kongres Československé společnosti mikrobiologické}. 2016. ISBN~978-80-270-0136-1.

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