HIF-1alpha Deficiency Attenuates the Cardiomyogenesis of Mouse
Embryonic Stem Cells

J 2016

HIF-1alpha Deficiency Attenuates the Cardiomyogenesis of Mouse Embryonic Stem Cells

KUDOVÁ, Jana; Jiřina PROCHÁZKOVÁ; Ondřej VAŠIČEK; Tomáš PEREČKO; Miroslava SEDLÁČKOVÁ et al.

Základní údaje

Originální název

HIF-1alpha Deficiency Attenuates the Cardiomyogenesis of Mouse Embryonic Stem Cells

Autoři

KUDOVÁ, Jana; Jiřina PROCHÁZKOVÁ ; Ondřej VAŠIČEK; Tomáš PEREČKO; Miroslava SEDLÁČKOVÁ; Martin PEŠL ; Jiří PACHERNÍK a Lukáš KUBALA

Vydání

Plos one, San Francisco, Public Library of Science, 2016, 1932-6203

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.806

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/16:00088134

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

INDUCIBLE FACTOR 1-ALPHA; GENE-EXPRESSION; CARDIAC DIFFERENTIATION; HIF-1-ALPHA; HEART; CARDIOMYOCYTES; MORPHOGENESIS; ENDODERM; ANGIOGENESIS; HIF-2-ALPHA

Štítky

EL OK, podil

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 12. 9. 2016 09:56, Ing. Mgr. Věra Pospíšilíková

Anotace

V originále

Cardiac cell formation, cardiomyogenesis, is critically dependent on oxygen availability. It is known that hypoxia, a reduced oxygen level, modulates the in vitro differentiation of pluripotent cells into cardiomyocytes via hypoxia inducible factor-1alpha (HIF-1 alpha)-dependent mechanisms. However, the direct impact of HIF-1 alpha deficiency on the formation and maturation of cardiac-like cells derived from mouse embryonic stem cells (mESC) in vitro remains to be elucidated. In the present study, we demonstrated that HIF-1 alpha deficiency significantly altered the quality and quantity of mESC-derived cardiomyocytes. It was accompanied with lower mRNA and protein levels of cardiac cell specific markers (myosin heavy chains 6 and 7) and with a decreasing percentage of myosin heavy chain alpha and beta, and cardiac troponin T-positive cells. As to structural aspects of the differentiated cardiomyocytes, the localization of contractile proteins (cardiac troponin T, myosin heavy chain alpha and beta) and the organization of myofibrils were also different. Simultaneously, HIF-1 alpha deficiency was associated with a lower percentage of beating embryoid bodies. Interestingly, an observed alteration in the in vitro differentiation scheme of HIF-1 alpha deficient cells was accompanied with significantly lower expression of the endodermal marker (hepatic nuclear factor 4 alpha). These findings thus suggest that HIF-1 alpha deficiency attenuates spontaneous cardiomyogenesis through the negative regulation of endoderm development in mESC differentiating in vitro.

Návaznosti

EE2.3.20.0185, projekt VaV

Název: Centrum analýz a modelování tkání a orgánů

GJ15-13443Y, projekt VaV

Název: Úloha hypoxií indukovaného faktoru 1 alfa ve vývoji populace neurálních kmenových buněk myši

Investor: Grantová agentura ČR, Úloha hypoxií indukovaného faktoru 1 alfa ve vývoji populace neurálních kmenových buněk myši

Citovat

KUDOVÁ, Jana; Jiřina PROCHÁZKOVÁ; Ondřej VAŠIČEK; Tomáš PEREČKO; Miroslava SEDLÁČKOVÁ; Martin PEŠL; Jiří PACHERNÍK a Lukáš KUBALA. HIF-1alpha Deficiency Attenuates the Cardiomyogenesis of Mouse Embryonic Stem Cells. Plos one. San Francisco: Public Library of Science, 2016, roč. 11, č. 6, s. 1-17. ISSN 1932-6203. Dostupné z: https://doi.org/10.1371/journal.pone.0158358.

@article{1353890,
   author = {Kudová, Jana and Procházková, Jiřina and Vašiček, Ondřej and Perečko, Tomáš and Sedláčková, Miroslava and Pešl, Martin and Pacherník, Jiří and Kubala, Lukáš},
   article_location = {San Francisco},
   article_number = {6},
   doi = {https://doi.org/10.1371/journal.pone.0158358},
   keywords = {INDUCIBLE FACTOR 1-ALPHA; GENE-EXPRESSION; CARDIAC DIFFERENTIATION; HIF-1-ALPHA; HEART; CARDIOMYOCYTES; MORPHOGENESIS; ENDODERM; ANGIOGENESIS; HIF-2-ALPHA},
   language = {eng},
   issn = {1932-6203},
   journal = {Plos one},
   title = {HIF-1alpha Deficiency Attenuates the Cardiomyogenesis of Mouse Embryonic Stem Cells},
   volume = {11},
   year = {2016}
}

TY  - JOUR
ID  - 1353890
AU  - Kudová, Jana - Procházková, Jiřina - Vašiček, Ondřej - Perečko, Tomáš - Sedláčková, Miroslava - Pešl, Martin - Pacherník, Jiří - Kubala, Lukáš
PY  - 2016
TI  - HIF-1alpha Deficiency Attenuates the Cardiomyogenesis of Mouse Embryonic Stem Cells
JF  - Plos one
VL  - 11
IS  - 6
SP  - 1-17
EP  - 1-17
PB  - Public Library of Science
SN  - 19326203
KW  - INDUCIBLE FACTOR 1-ALPHA
KW  - GENE-EXPRESSION
KW  - CARDIAC DIFFERENTIATION
KW  - HIF-1-ALPHA
KW  - HEART
KW  - CARDIOMYOCYTES
KW  - MORPHOGENESIS
KW  - ENDODERM
KW  - ANGIOGENESIS
KW  - HIF-2-ALPHA
N2  - Cardiac cell formation, cardiomyogenesis, is critically dependent on oxygen availability. It is known that hypoxia, a reduced oxygen level, modulates the in vitro differentiation of pluripotent cells into cardiomyocytes via hypoxia inducible factor-1alpha (HIF-1 alpha)-dependent mechanisms. However, the direct impact of HIF-1 alpha deficiency on the formation and maturation of cardiac-like cells derived from mouse embryonic stem cells (mESC) in vitro remains to be elucidated. In the present study, we demonstrated that HIF-1 alpha deficiency significantly altered the quality and quantity of mESC-derived cardiomyocytes. It was accompanied with lower mRNA and protein levels of cardiac cell specific markers (myosin heavy chains 6 and 7) and with a decreasing percentage of myosin heavy chain alpha and beta, and cardiac troponin T-positive cells. As to structural aspects of the differentiated cardiomyocytes, the localization of contractile proteins (cardiac troponin T, myosin heavy chain alpha and beta) and the organization of myofibrils were also different. Simultaneously, HIF-1 alpha deficiency was associated with a lower percentage of beating embryoid bodies. Interestingly, an observed alteration in the in vitro differentiation scheme of HIF-1 alpha deficient cells was accompanied with significantly lower expression of the endodermal marker (hepatic nuclear factor 4 alpha). These findings thus suggest that HIF-1 alpha deficiency attenuates spontaneous cardiomyogenesis through the negative regulation of endoderm development in mESC differentiating in vitro.
ER  -

KUDOVÁ, Jana; Jiřina PROCHÁZKOVÁ; Ondřej VAŠIČEK; Tomáš PEREČKO; Miroslava SEDLÁČKOVÁ; Martin PEŠL; Jiří PACHERNÍK a Lukáš KUBALA. HIF-1alpha Deficiency Attenuates the Cardiomyogenesis of Mouse Embryonic Stem Cells. \textit{Plos one}. San Francisco: Public Library of Science, 2016, roč.~11, č.~6, s.~1-17. ISSN~1932-6203. Dostupné z: https://doi.org/10.1371/journal.pone.0158358.

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