Cytokinin controls cell differentiation via negative regulation
of secondary cell wall master regulators NST1 and NST3

k 2015

Cytokinin controls cell differentiation via negative regulation of secondary cell wall master regulators NST1 and NST3

DIDI, Vojtěch, Jana VAŠÍČKOVÁ, Radek JUPA, Mariana BENITEZ KEINRAD, Tereza DOBISOVÁ et. al.

Základní údaje

Originální název

Cytokinin controls cell differentiation via negative regulation of secondary cell wall master regulators NST1 and NST3

Autoři

DIDI, Vojtěch (203 Česká republika, domácí), Jana VAŠÍČKOVÁ (203 Česká republika), Radek JUPA (203 Česká republika), Mariana BENITEZ KEINRAD (484 Mexiko), Tereza DOBISOVÁ (203 Česká republika), Radim ČEGAN (203 Česká republika), Roman HOBZA (203 Česká republika), Shawn MANSFIELD (124 Kanada), Vít GLOSER (203 Česká republika) a Jan HEJÁTKO (203 Česká republika, garant)

Vydání

Signalling in plant development - EMBO conference, 2015

Další údaje

Jazyk

angličtina

Typ výsledku

Prezentace na konferencích

Obor

Genetika a molekulární biologie

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14740/15:00088171

Organizační jednotka

Středoevropský technologický institut

Klíčová slova česky

sekundární buněčná stěna; cytokinin

Klíčová slova anglicky

Secondary cell wall; cytokinin

Příznaky

Mezinárodní význam

Změněno: 21. 9. 2016 12:45, Mgr. Vojtěch Didi, Ph.D.

Anotace

V originále

Control over the equilibrium between cell division and cell differentiation represents the core developmental regulation across all kingdoms Plant hormones cytokinins were shown to regulate both the process of cell differentiation and cell proliferation. However, the molecular mechanism of cytokinin-controlled cell differentiation is far from being understood. Here we present the role of cytokinins in the control of differentiation program of secondary cell wall (SCW)-containing cells. We found that impaired cytokinin signaling disturbs apical/basal developmental gradient in the inflorescence stem of Arabidopsis. Attenuated cytokinin signaling results in formation of vascular bundles with changed protoxylem/metaxylem ratio and formation of tracheary elements (TEs) with reduced diameter and dramatically reduced water conductance. Increased lignification in both TEs and xylem fibers suggested early cell differentiation. The genome-wide next-gen transcriptional profiling revealed ectopic upregulation of factors throughout the hierarchical transcriptional cascade including the NAC TFs NST1 and SND1/NST3, the master transcriptional switches of SCW. That leads to precocious activation of downstream structural components mediating the biosynthesis of xylan, lignin and cellulose. We found that disturbing the apical/basal cytokinin distribution is responsible for ectopic upregulation of NST1 and NST3 master regulators as well as downstream structural genes involved in SCW. Cytokinin biosynthgesis atipt1,3,5,7 mutant phenocopies the early differentiation phenotype and functional defects of tracheary elements Exogenous cytokinin application was able to downregulate the ectopic increase in NST1/NST3 expression and rescue the precocious differentiation phenotype in atipt1,3,5,7. Effect of cytokinin on SCW differentiation was abolished in nst1/nst3 mutant background. The negative role of cytokinins in regulation of NSTs expression was confirmed by qRT PCR and downregulation of pNST3:NST3-GUS expression by exogenous cytokinins. Based on our findings we propose the role of cytokinins as a positional cue controlling cell differentiation status via regulating expression of SCW master regulators NST1 and NST3.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

GA13-25280S, projekt VaV

Název: Elucidating molecular mechanisms of cytokinins-ethylene crosstalk in the plant development

Investor: Grantová agentura ČR, Elucidating molecular mechanisms of cytokinins-ethylene crosstalk in the plant development

Citovat

DIDI, Vojtěch, Jana VAŠÍČKOVÁ, Radek JUPA, Mariana BENITEZ KEINRAD, Tereza DOBISOVÁ, Radim ČEGAN, Roman HOBZA, Shawn MANSFIELD, Vít GLOSER a Jan HEJÁTKO. Cytokinin controls cell differentiation via negative regulation of secondary cell wall master regulators NST1 and NST3. In Signalling in plant development - EMBO conference. 2015.

@proceedings{1354634,
   author = {Didi, Vojtěch and Vašíčková, Jana and Jupa, Radek and Benitez Keinrad, Mariana and Dobisová, Tereza and Čegan, Radim and Hobza, Roman and Mansfield, Shawn and Gloser, Vít and Hejátko, Jan},
   booktitle = {Signalling in plant development - EMBO conference},
   keywords = {Secondary cell wall; cytokinin},
   language = {eng},
   title = {Cytokinin controls cell differentiation via negative regulation of secondary cell wall master regulators NST1 and NST3},
   year = {2015}
}

TY  - CONF
ID  - 1354634
AU  - Didi, Vojtěch - Vašíčková, Jana - Jupa, Radek - Benitez Keinrad, Mariana - Dobisová, Tereza - Čegan, Radim - Hobza, Roman - Mansfield, Shawn - Gloser, Vít - Hejátko, Jan
PY  - 2015
TI  - Cytokinin controls cell differentiation via negative regulation of secondary cell wall master regulators NST1 and NST3
KW  - Secondary cell wall
KW  - cytokinin
N2  - Control over the equilibrium between cell division and cell differentiation represents the core developmental regulation across all kingdoms Plant hormones cytokinins were shown to regulate both the process of cell differentiation and cell proliferation. However, the molecular mechanism of cytokinin-controlled cell differentiation is far from being understood. Here we present the role of cytokinins in the control of differentiation program of secondary cell wall (SCW)-containing cells. We found that impaired cytokinin signaling disturbs apical/basal developmental gradient in the inflorescence stem of Arabidopsis. Attenuated cytokinin signaling results in formation of vascular bundles with changed protoxylem/metaxylem ratio and formation of tracheary elements (TEs) with reduced diameter and dramatically reduced water conductance. Increased lignification in both TEs and xylem fibers suggested early cell differentiation. The genome-wide next-gen transcriptional profiling revealed ectopic upregulation of factors throughout the hierarchical transcriptional cascade including the NAC TFs NST1 and SND1/NST3, the master transcriptional switches of SCW. That leads to precocious activation of downstream structural components mediating the biosynthesis of xylan, lignin and cellulose. We found that disturbing the apical/basal cytokinin distribution is responsible for ectopic upregulation of NST1 and NST3 master regulators as well as downstream structural genes involved in SCW. Cytokinin biosynthgesis atipt1,3,5,7 mutant phenocopies the early differentiation phenotype and functional defects of tracheary elements Exogenous cytokinin application was able to downregulate the ectopic increase in NST1/NST3 expression and rescue the precocious differentiation phenotype in atipt1,3,5,7. Effect of cytokinin on SCW differentiation was abolished in nst1/nst3 mutant background. The negative role of cytokinins in regulation of NSTs expression was confirmed by qRT PCR and downregulation of pNST3:NST3-GUS expression by exogenous cytokinins. Based on our findings we propose the role of cytokinins as a positional cue controlling cell differentiation status via regulating expression of SCW master regulators NST1 and NST3.
ER  -

DIDI, Vojtěch, Jana VAŠÍČKOVÁ, Radek JUPA, Mariana BENITEZ KEINRAD, Tereza DOBISOVÁ, Radim ČEGAN, Roman HOBZA, Shawn MANSFIELD, Vít GLOSER a Jan HEJÁTKO. Cytokinin controls cell differentiation via negative regulation of secondary cell wall master regulators NST1 and NST3. In \textit{Signalling in plant development - EMBO conference}. 2015.

Podrobný výpis o publikaci