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@article{1354651,
author = {Pizzetti, Ilaria and Schulz, Frederik and Tyml, Tomáš and Fuchs, Bernhard M. and Amann, Rudolf and Horn, Matthias and Fazi, Stefano},
article_location = {Hoboken},
article_number = {8},
doi = {http://dx.doi.org/10.1111/1462-2920.13111},
keywords = {BAYESIAN PHYLOGENETIC INFERENCE; TARGETED OLIGONUCLEOTIDE PROBES; IN-SITU HYBRIDIZATION; WATER-TREATMENT-PLANT; ABORTED BOVINE FETUS; RIBOSOMAL-RNA GENE; ORDER CHLAMYDIALES; ENVIRONMENTAL CHLAMYDIAE; INTRACELLULAR BACTERIA; FAMILY CHLAMYDIACEAE},
language = {eng},
issn = {1462-2912},
journal = {Environmental Microbiology},
title = {Chlamydial seasonal dynamics and isolation of ‘Candidatus Neptunochlamydia vexilliferae’from a Tyrrhenian coastal lake.},
url = {http://onlinelibrary.wiley.com/doi/10.1111/1462-2920.13111/full},
volume = {18},
year = {2016}
}
TY - JOUR
ID - 1354651
AU - Pizzetti, Ilaria - Schulz, Frederik - Tyml, Tomáš - Fuchs, Bernhard M. - Amann, Rudolf - Horn, Matthias - Fazi, Stefano
PY - 2016
TI - Chlamydial seasonal dynamics and isolation of ‘Candidatus Neptunochlamydia vexilliferae’from a Tyrrhenian coastal lake.
JF - Environmental Microbiology
VL - 18
IS - 8
SP - 2405-2417
EP - 2405-2417
PB - WILEY-BLACKWELL
SN - 14622912
KW - BAYESIAN PHYLOGENETIC INFERENCE
KW - TARGETED OLIGONUCLEOTIDE PROBES
KW - IN-SITU HYBRIDIZATION
KW - WATER-TREATMENT-PLANT
KW - ABORTED BOVINE FETUS
KW - RIBOSOMAL-RNA GENE
KW - ORDER CHLAMYDIALES
KW - ENVIRONMENTAL CHLAMYDIAE
KW - INTRACELLULAR BACTERIA
KW - FAMILY CHLAMYDIACEAE
UR - http://onlinelibrary.wiley.com/doi/10.1111/1462-2920.13111/full
N2 - The Chlamydiae are a phylum of obligate intracellular bacteria comprising important human and animal pathogens, yet their occurrence in the environment, their phylogenetic diversity and their host range has been largely underestimated. We investigated the seasonality of environmental chlamydiae in a Tyrrhenian coastal lake. By catalysed reporter deposition fluorescence in situ hybridization, we quantified the small planktonic cells and detected a peak in the abundance of environmental chlamydiae in early autumn with up to 5.9x10(4) cells ml(-1). Super-resolution microscopy improved the visualization and quantification of these bacteria and enabled the detection of pleomorphic chlamydial cells in their protist host directly in an environmental sample. To isolate environmental chlamydiae together with their host, we applied a high-throughput limited dilution approach and successfully recovered a Vexillifera sp., strain harbouring chlamydiae (93% 16S rRNA sequence identity to Simkania negevensis), tentatively named CandidatusNeptunochlamydia vexilliferae'. Transmission electron microscopy in combination with fluorescence in situ hybridization was used to prove the intracellular location of these bacteria representing the first strain of marine chlamydiae stably maintained alongside with their host in a laboratory culture. Taken together, this study contributes to a better understanding of the distribution and diversity of environmental chlamydiae in previously neglected marine environments.
ER -
PIZZETTI, Ilaria, Frederik SCHULZ, Tomáš TYML, Bernhard M. FUCHS, Rudolf AMANN, Matthias HORN a Stefano FAZI. Chlamydial seasonal dynamics and isolation of ‘Candidatus Neptunochlamydia vexilliferae’from a Tyrrhenian coastal lake. \textit{Environmental Microbiology}. Hoboken: WILEY-BLACKWELL, 2016, roč.~18, č.~8, s.~2405-2417. ISSN~1462-2912. Dostupné z: https://dx.doi.org/10.1111/1462-2920.13111.
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