Prostate tumor attenuation in the nu/nu murine model due to
anti-sarcosine antibodies in folate-targeted liposomes

J 2016

Prostate tumor attenuation in the nu/nu murine model due to anti-sarcosine antibodies in folate-targeted liposomes

HEGER, Zbynek; Hana POLANSKÁ; Miguel Angel Merlos RODRIGO; Roman GURÁŇ; Pavel KULICH et al.

Základní údaje

Originální název

Prostate tumor attenuation in the nu/nu murine model due to anti-sarcosine antibodies in folate-targeted liposomes

Autoři

HEGER, Zbynek; Hana POLANSKÁ; Miguel Angel Merlos RODRIGO; Roman GURÁŇ; Pavel KULICH; Pavel KOPEL; Michal MASAŘÍK; Tomas ECKSCHLAGER; Marie STIBOROVA; Rene KIZEK a Vojtech ADAM

Vydání

Scientific Reports, London, Nature Publishing Group, 2016, 2045-2322

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30200 3.2 Clinical medicine

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.259

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/16:00090895

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

CANCER PROGRESSION; CELL-LINE; IN-VITRO; METALLOTHIONEIN; EXPRESSION; THERAPY; PROTEIN; URINE; NANOPARTICLES; TRAFFICKING

Štítky

EL OK

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 12. 10. 2016 12:17, Ing. Mgr. Věra Pospíšilíková

Anotace

V originále

Herein, we describe the preparation of liposomes with folate-targeting properties for the encapsulation of anti-sarcosine antibodies (antisarAbs@LIP) and sarcosine (sar@LIP). The competitive inhibitory effects of exogenously added folic acid supported the role of folate targeting in liposome internalization. We examined the effects of repeated administration on mice PC-3 xenografts. Sar@LIP treatment significantly increased tumor volume and weight compared to controls treated with empty liposomes. Moreover, antisarAbs@LIP administration exhibited a mild antitumor effect. We also identified differences in gene expression patterns post-treatment. Furthermore, Sar@LIP treatment resulted in decreased amounts of tumor zinc ions and total metallothioneins. Examination of the spatial distribution across the tumor sections revealed a sarcosine-related decline of the MT1X isoform within the marginal regions but an elevation after antisarAbs@LIP administration. Our exploratory results demonstrate the importance of sarcosine as an oncometabolite in PCa. Moreover, we have shown that sarcosine can be a potential target for anticancer strategies in management of PCa.

Návaznosti

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Přiložené soubory

srep33379.pdf

Citovat

HEGER, Zbynek; Hana POLANSKÁ; Miguel Angel Merlos RODRIGO; Roman GURÁŇ; Pavel KULICH; Pavel KOPEL; Michal MASAŘÍK; Tomas ECKSCHLAGER; Marie STIBOROVA; Rene KIZEK a Vojtech ADAM. Prostate tumor attenuation in the nu/nu murine model due to anti-sarcosine antibodies in folate-targeted liposomes. Scientific Reports. London: Nature Publishing Group, 2016, roč. 6, č. 33379, s. 1-11. ISSN 2045-2322. Dostupné z: https://doi.org/10.1038/srep33379.

@article{1354692,
   author = {Heger, Zbynek and Polanská, Hana and Rodrigo, Miguel Angel Merlos and Guráň, Roman and Kulich, Pavel and Kopel, Pavel and Masařík, Michal and Eckschlager, Tomas and Stiborova, Marie and Kizek, Rene and Adam, Vojtech},
   article_location = {London},
   article_number = {33379},
   doi = {https://doi.org/10.1038/srep33379},
   keywords = {CANCER PROGRESSION; CELL-LINE; IN-VITRO; METALLOTHIONEIN; EXPRESSION; THERAPY; PROTEIN; URINE; NANOPARTICLES; TRAFFICKING},
   language = {eng},
   issn = {2045-2322},
   journal = {Scientific Reports},
   title = {Prostate tumor attenuation in the nu/nu murine model due to anti-sarcosine antibodies in folate-targeted liposomes},
   url = {http://www.nature.com/articles/srep33379},
   volume = {6},
   year = {2016}
}

TY  - JOUR
ID  - 1354692
AU  - Heger, Zbynek - Polanská, Hana - Rodrigo, Miguel Angel Merlos - Guráň, Roman - Kulich, Pavel - Kopel, Pavel - Masařík, Michal - Eckschlager, Tomas - Stiborova, Marie - Kizek, Rene - Adam, Vojtech
PY  - 2016
TI  - Prostate tumor attenuation in the nu/nu murine model due to anti-sarcosine antibodies in folate-targeted liposomes
JF  - Scientific Reports
VL  - 6
IS  - 33379
SP  - 1-11
EP  - 1-11
PB  - Nature Publishing Group
SN  - 20452322
KW  - CANCER PROGRESSION
KW  - CELL-LINE
KW  - IN-VITRO
KW  - METALLOTHIONEIN
KW  - EXPRESSION
KW  - THERAPY
KW  - PROTEIN
KW  - URINE
KW  - NANOPARTICLES
KW  - TRAFFICKING
UR  - http://www.nature.com/articles/srep33379
N2  - Herein, we describe the preparation of liposomes with folate-targeting properties for the encapsulation of anti-sarcosine antibodies (antisarAbs@LIP) and sarcosine (sar@LIP). The competitive inhibitory effects of exogenously added folic acid supported the role of folate targeting in liposome internalization. We examined the effects of repeated administration on mice PC-3 xenografts. Sar@LIP treatment significantly increased tumor volume and weight compared to controls treated with empty liposomes. Moreover, antisarAbs@LIP administration exhibited a mild antitumor effect. We also identified differences in gene expression patterns post-treatment. Furthermore, Sar@LIP treatment resulted in decreased amounts of tumor zinc ions and total metallothioneins. Examination of the spatial distribution across the tumor sections revealed a sarcosine-related decline of the MT1X isoform within the marginal regions but an elevation after antisarAbs@LIP administration. Our exploratory results demonstrate the importance of sarcosine as an oncometabolite in PCa. Moreover, we have shown that sarcosine can be a potential target for anticancer strategies in management of PCa.
ER  -

HEGER, Zbynek; Hana POLANSKÁ; Miguel Angel Merlos RODRIGO; Roman GURÁŇ; Pavel KULICH; Pavel KOPEL; Michal MASAŘÍK; Tomas ECKSCHLAGER; Marie STIBOROVA; Rene KIZEK a Vojtech ADAM. Prostate tumor attenuation in the nu/nu murine model due to anti-sarcosine antibodies in folate-targeted liposomes. \textit{Scientific Reports}. London: Nature Publishing Group, 2016, roč.~6, č.~33379, s.~1-11. ISSN~2045-2322. Dostupné z: https://doi.org/10.1038/srep33379.

Přehled o publikaci