Nitro-oleic acid inhibits vascular endothelial inflammatory
responses and the endothelial-mesenchymal transition

J 2016

Nitro-oleic acid inhibits vascular endothelial inflammatory responses and the endothelial-mesenchymal transition

AMBROŽOVÁ, Gabriela; Táňa FIDLEROVÁ; Hana VEREŠČÁKOVÁ; Adolf KOUDELKA; T.K. RUDOLPH et al.

Základní údaje

Originální název

Nitro-oleic acid inhibits vascular endothelial inflammatory responses and the endothelial-mesenchymal transition

Autoři

AMBROŽOVÁ, Gabriela; Táňa FIDLEROVÁ; Hana VEREŠČÁKOVÁ; Adolf KOUDELKA; T.K. RUDOLPH; S.R. WOODCOCK; B.A. FREEMAN; Lukáš KUBALA a Michaela PEKAROVÁ

Vydání

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, AMSTERDAM, ELSEVIER SCIENCE BV, 2016, 0304-4165

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.702

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/16:00095883

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Nitro-fatty acids; Nitro-oleic acid; Endothelial cells; Macrophages; Vascular inflammation; Endothelial-mesenchymal transition

Štítky

NZ, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 13. 4. 2018 14:42, Ing. Nicole Zrilić

Anotace

V originále

Background: Inflammatory-mediated pathological processes in the endothelium arise as a consequence of the dysregulation of vascular homeostasis. Of particular importance are mediators produced by stimulated monocytes/macrophages inducing activation of endothelial cells (ECs). This is manifested by excessive soluble pro-inflammatory mediator production and cell surface adhesion molecule expression. Nitro-fatty acids are endogenous products of metabolic and inflammatory reactions that display immuno-regulatory potential and may represent a novel therapeutic strategy to treat inflammatory diseases. The purpose of our study was to characterize the effects of nitro-oleic acid (OA-NO2) on inflammatory responses and the endothelial-mesenchymal transition (EndMT) in ECs that is a consequence of the altered healing phase of the immune response. Methods: The effect of OA-NO2 on inflammatory responses and EndMT was determined in murine macrophages and murine and human ECs using Western blotting, ELISA, immunostaining, and functional assays. Results: OA-NO2 limited the activation of macrophages and ECs by reducing pro-inflammatory cytokine production and adhesion molecule expression through its modulation of STAT. MAPK and NF-kappa B-regulated signaling. OA-NO2 also decreased transforming growth factor-beta-stimulated EndMT and pro-fibrotic phenotype of ECs. These effects are related to the downregulation of Smad2/3. Conclusions: The study shows the pleiotropic effect of OA-NO2 on regulating EC-macrophage interactions during the immune response and suggests a role for OA-NO2 in the regulation of vascular endothelial immune and fibrotic responses arising during chronic inflammation. General significance: These findings propose the OA-NO2 may be useful as a novel therapeutic agent for treatment of cardiovascular disorders associated with dysregulation of the endothelial immune response. (C) 2016 Elsevier B.V. All rights reserved.

Citovat

AMBROŽOVÁ, Gabriela; Táňa FIDLEROVÁ; Hana VEREŠČÁKOVÁ; Adolf KOUDELKA; T.K. RUDOLPH; S.R. WOODCOCK; B.A. FREEMAN; Lukáš KUBALA a Michaela PEKAROVÁ. Nitro-oleic acid inhibits vascular endothelial inflammatory responses and the endothelial-mesenchymal transition. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS. AMSTERDAM: ELSEVIER SCIENCE BV, 2016, roč. 1860, č. 11, s. 2428-2437. ISSN 0304-4165. Dostupné z: https://doi.org/10.1016/j.bbagen.2016.07.010.

@article{1355491,
   author = {Ambrožová, Gabriela and Fidlerová, Táňa and Vereščáková, Hana and Koudelka, Adolf and Rudolph, T.K. and Woodcock, S.R. and Freeman, B.A. and Kubala, Lukáš and Pekarová, Michaela},
   article_location = {AMSTERDAM},
   article_number = {11},
   doi = {https://doi.org/10.1016/j.bbagen.2016.07.010},
   keywords = {Nitro-fatty acids; Nitro-oleic acid; Endothelial cells; Macrophages; Vascular inflammation; Endothelial-mesenchymal transition},
   language = {eng},
   issn = {0304-4165},
   journal = {BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS},
   title = {Nitro-oleic acid inhibits vascular endothelial inflammatory responses and the endothelial-mesenchymal transition},
   url = {https://www.ncbi.nlm.nih.gov/pubmed/27431604},
   volume = {1860},
   year = {2016}
}

TY  - JOUR
ID  - 1355491
AU  - Ambrožová, Gabriela - Fidlerová, Táňa - Vereščáková, Hana - Koudelka, Adolf - Rudolph, T.K. - Woodcock, S.R. - Freeman, B.A. - Kubala, Lukáš - Pekarová, Michaela
PY  - 2016
TI  - Nitro-oleic acid inhibits vascular endothelial inflammatory responses and the endothelial-mesenchymal transition
JF  - BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
VL  - 1860
IS  - 11
SP  - 2428-2437
EP  - 2428-2437
PB  - ELSEVIER SCIENCE BV
SN  - 03044165
KW  - Nitro-fatty acids
KW  - Nitro-oleic acid
KW  - Endothelial cells
KW  - Macrophages
KW  - Vascular inflammation
KW  - Endothelial-mesenchymal transition
UR  - https://www.ncbi.nlm.nih.gov/pubmed/27431604
L2  - https://www.ncbi.nlm.nih.gov/pubmed/27431604
N2  - Background: Inflammatory-mediated pathological processes in the endothelium arise as a consequence of the dysregulation of vascular homeostasis. Of particular importance are mediators produced by stimulated monocytes/macrophages inducing activation of endothelial cells (ECs). This is manifested by excessive soluble pro-inflammatory mediator production and cell surface adhesion molecule expression. Nitro-fatty acids are endogenous products of metabolic and inflammatory reactions that display immuno-regulatory potential and may represent a novel therapeutic strategy to treat inflammatory diseases. The purpose of our study was to characterize the effects of nitro-oleic acid (OA-NO2) on inflammatory responses and the endothelial-mesenchymal transition (EndMT) in ECs that is a consequence of the altered healing phase of the immune response. Methods: The effect of OA-NO2 on inflammatory responses and EndMT was determined in murine macrophages and murine and human ECs using Western blotting, ELISA, immunostaining, and functional assays. Results: OA-NO2 limited the activation of macrophages and ECs by reducing pro-inflammatory cytokine production and adhesion molecule expression through its modulation of STAT. MAPK and NF-kappa B-regulated signaling. OA-NO2 also decreased transforming growth factor-beta-stimulated EndMT and pro-fibrotic phenotype of ECs. These effects are related to the downregulation of Smad2/3. Conclusions: The study shows the pleiotropic effect of OA-NO2 on regulating EC-macrophage interactions during the immune response and suggests a role for OA-NO2 in the regulation of vascular endothelial immune and fibrotic responses arising during chronic inflammation. General significance: These findings propose the OA-NO2 may be useful as a novel therapeutic agent for treatment of cardiovascular disorders associated with dysregulation of the endothelial immune response. (C) 2016 Elsevier B.V. All rights reserved.
ER  -

AMBROŽOVÁ, Gabriela; Táňa FIDLEROVÁ; Hana VEREŠČÁKOVÁ; Adolf KOUDELKA; T.K. RUDOLPH; S.R. WOODCOCK; B.A. FREEMAN; Lukáš KUBALA a Michaela PEKAROVÁ. Nitro-oleic acid inhibits vascular endothelial inflammatory responses and the endothelial-mesenchymal transition. \textit{BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS}. AMSTERDAM: ELSEVIER SCIENCE BV, 2016, roč.~1860, č.~11, s.~2428-2437. ISSN~0304-4165. Dostupné z: https://doi.org/10.1016/j.bbagen.2016.07.010.

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