2016
Student scholarship
ŠKORPÍKOVÁ, LucieZákladní údaje
Originální název
Student scholarship
Autoři
Vydání
2016
Nakladatel
EMOP XII – the 12th European Multicolloquium of Parasitology
Další údaje
Jazyk
angličtina
Typ výsledku
Ocenění vědecké a umělecké činnosti
Obor
10600 1.6 Biological sciences
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Označené pro přenos do RIV
Ne
Organizační jednotka
Přírodovědecká fakulta
Klíčová slova česky
xMAP technologie, imunodiagnostika, cystatiny, inhibitory cysteinových peptidáz, Trichinella
Klíčová slova anglicky
xMAP technology, immunodiagnostics, cystatins, inhibitors of cysteine peptidase, Trichinella
Změněno: 9. 10. 2016 20:34, Mgr. Lucie Škorpíková, Ph.D.
Anotace
V originále
Roundworms of the genus Trichinella are clinically important parasites invading cells of the small intestine and skeletal muscles. They infect a broad range of hosts involving birds, reptiles and mammals. Seven of twelve genotypes of Trichinella spp. are zoonoses causing the trichinellosis - a serious human disease. During the life cycle, these parasites release variety of functional molecules, which are mainly present in excretory-secretory (E-S) products. They are essential for many biological processes like migration, digestion, development, and also in manipulation of the host immunne system. The dominant components of the E-S products are cystatins - reversible inhibitors of cysteine peptidases of C1 (papain-like) family and rarely of C13 (legumain-like) family. All the representatives of cystatin superfamily are evolutionary related and share some sequential and structural identity. Our work is focused on the characterization of "multi cystatin-like domain" (mcd-1) genes and proteins of Trichinella spiralis and T. pseudospiralis. The complete sequences of genes were obtained and recombinant analogs of MCD-1 proteins were prepared in Escherichia coli expression system. Currently, the recombinant MCD-1 molecules are used also in regard to develop the reliable molecular method useful for serological quantitative and qualitative diagnostics of Trichinella. We employed the xMAP technology - novel diagnostic platform based on magnetic microspheres and a signal evaluation by MAGPIX device.
Návaznosti
| MUNI/A/1325/2015, interní kód MU |
|