| KRBKOVÁ, Lenka, Lukáš HOMOLA, Adriana BRAUNOVÁ, Peter MIKOLÁŠEK, Jana BEDNÁŘOVÁ a Zdeňka ČERMÁKOVÁ. Assessment of antibodies against surface and outer membrane proteins of Anaplasma phagocytophilum in Lyme borreliosis and tick-borne encephalitis paediatric patients. Epidemiology and infection. Cambridge: Cambridge University Press, 2016, roč. 144, č. 12, s. 2597-2604. ISSN 0950-2688. Dostupné z: https://dx.doi.org/10.1017/S0950268816000972. |
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@article{1356783,
author = {Krbková, Lenka and Homola, Lukáš and Braunová, Adriana and Mikolášek, Peter and Bednářová, Jana and Čermáková, Zdeňka},
article_location = {Cambridge},
article_number = {12},
doi = {http://dx.doi.org/10.1017/S0950268816000972},
keywords = {Anaplasma phagocytophilum; human granulocytic anaplasmosis; Lyme borreliosis; major surface proteins; outer membrane protein A; tick-borne encephalitis},
language = {eng},
issn = {0950-2688},
journal = {Epidemiology and infection},
title = {Assessment of antibodies against surface and outer membrane proteins of Anaplasma phagocytophilum in Lyme borreliosis and tick-borne encephalitis paediatric patients},
volume = {144},
year = {2016}
}
TY - JOUR
ID - 1356783
AU - Krbková, Lenka - Homola, Lukáš - Braunová, Adriana - Mikolášek, Peter - Bednářová, Jana - Čermáková, Zdeňka
PY - 2016
TI - Assessment of antibodies against surface and outer membrane proteins of Anaplasma phagocytophilum in Lyme borreliosis and tick-borne encephalitis paediatric patients
JF - Epidemiology and infection
VL - 144
IS - 12
SP - 2597-2604
EP - 2597-2604
PB - Cambridge University Press
SN - 09502688
KW - Anaplasma phagocytophilum
KW - human granulocytic anaplasmosis
KW - Lyme borreliosis
KW - major surface proteins
KW - outer membrane protein A
KW - tick-borne encephalitis
N2 - To examine evidence of positive antibodies against immunogenic proteins of Anaplasma phagocytophilum in patients with other tick-borne infections and to diagnose possible co-infections, 412 serum specimens were tested by immunoblotting using three specific Anaplasma antigens: surface proteins p44 and Asp62 and outer membrane protein A (OmpA). In total, 284 serum samples from children with Lyme borreliosis and 12 serum samples from children with tick-borne encephalitis were tested. Sera from patients with viral aseptic meningitis (n = 47) and from blood donors (n = 69) were used as controls. Among all serum specimens from patients with tick-borne infections submitted for this study, six samples (20%) showed positive IgM reactions and seven samples (24%) were IgG positive for A. phagocytophilum by immunoblot. Borderline reactivity was found in 30 samples (1014%) for IgM and 36 samples (122%) for IgG. The difference between patients and blood donors was statistically significant for IgM (P = 0006) and for IgG (P = 00007) antibodies. A statistically significant result was obtained for IgG (P = 002) but not for IgM between patients and children with aseptic meningitis. Immunoblot using three specific antigens provides novel information about the positivity of antibodies to A. phagocytophilum in children with other tick-borne infections. Taking into account clinical and laboratory findings of children despite antibody positivity, no case of human granulocytic anaplasmosis was demonstrated.
ER -
KRBKOVÁ, Lenka, Lukáš HOMOLA, Adriana BRAUNOVÁ, Peter MIKOLÁŠEK, Jana BEDNÁŘOVÁ a Zdeňka ČERMÁKOVÁ. Assessment of antibodies against surface and outer membrane proteins of Anaplasma phagocytophilum in Lyme borreliosis and tick-borne encephalitis paediatric patients. \textit{Epidemiology and infection}. Cambridge: Cambridge University Press, 2016, roč.~144, č.~12, s.~2597-2604. ISSN~0950-2688. Dostupné z: https://dx.doi.org/10.1017/S0950268816000972.
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