Další formáty:
BibTeX
LaTeX
RIS
@article{1356794, author = {Bosák, Juraj and Micenková, Lenka and Doležalová, Magda and Šmajs, David}, article_location = {Jena}, article_number = {7}, doi = {http://dx.doi.org/10.1016/j.ijmm.2016.07.002}, keywords = {Colicin U; Colicin Y; Colicin-receptor interaction; Escherichia coli; OmpA}, language = {eng}, issn = {1438-4221}, journal = {International Journal of Medical Microbiology}, title = {Colicins U and Y inhibit growth of Escherichia coli strains via recognition of conserved OmpA extracellular loop 1}, volume = {306}, year = {2016} }
TY - JOUR ID - 1356794 AU - Bosák, Juraj - Micenková, Lenka - Doležalová, Magda - Šmajs, David PY - 2016 TI - Colicins U and Y inhibit growth of Escherichia coli strains via recognition of conserved OmpA extracellular loop 1 JF - International Journal of Medical Microbiology VL - 306 IS - 7 SP - 486-494 EP - 486-494 PB - Elsevier GMBH SN - 14384221 KW - Colicin U KW - Colicin Y KW - Colicin-receptor interaction KW - Escherichia coli KW - OmpA N2 - Interactions of colicins U and Y with the OmpA (Outer membrane protein A) receptor molecule were studied using site-directed mutagenesis and colicin binding assay. A systematic mutagenesis of the colicin-susceptible OmpA sequence from Escherichia coli (OmpAEC) to the colicin-resistant OmpA sequence from Serratia marcescens (OmpASM) was performed in regions corresponding to extracellular OmpA loops 1-4. Susceptibility to colicins U and Y was significantly affected by the OmpA mutation in loop 1. As with functional analysis, a decrease in binding capacity of His-tagged colicin U was found for recombinant OmpA with a mutated segment in loop 1 compared to control OmpAEC. To verify the importance of the identified amino acid residues in OmpA loop 1, we introduced loop 1 from OmpAEC into OmpASM, which resulted in the substantial increase of susceptibility to colicins U and Y. In addition, colicins U and Y were tested against a panel of 118 bacteriocin non-producing strains of four Escherichia species, including E. coli (39 strains), E. fergusonii (10 strains), E. hermannii (42 strains), and E. vulneris (27 strains). A majority (82%) of E. coli strains was susceptible to colicins U and Y. Interestingly, colicins U and Y also inhibited all of the 30 tested multidrug-resistant E. coli O25b-ST131 isolates. These findings, together with the fact that OmpA loop 1 is important for bacterial virulence and is evolutionary conserved, offer the potential of using colicins U and Y as specific anti-OmpA loop 1 directed antibacterial proteins. ER -
BOSÁK, Juraj, Lenka MICENKOVÁ, Magda DOLEŽALOVÁ a David ŠMAJS. Colicins U and Y inhibit growth of Escherichia coli strains via recognition of conserved OmpA extracellular loop 1. \textit{International Journal of Medical Microbiology}. Jena: Elsevier GMBH, 2016, roč.~306, č.~7, s.~486-494. ISSN~1438-4221. Dostupné z: https://dx.doi.org/10.1016/j.ijmm.2016.07.002.
|