Polymorphisms in genes encoding purinoreceptor and osteoprotegerin and external apical root resorption in children after orthodontic treatment

VRANKOVÁ, Zuzana, Martina SIROTKOVÁ, Petra BOŘILOVÁ LINHARTOVÁ, Pavlína ČERNOCHOVÁ, Jakub KAŠTOVSKÝ and Lydie IZAKOVIČOVÁ HOLLÁ. Polymorphisms in genes encoding purinoreceptor and osteoprotegerin and external apical root resorption in children after orthodontic treatment. In 15th Euro Congress on Dental & Oral Health, Rome. 2016. Available from: https://dx.doi.org/10.4172/2161-1122.C1.006.

Basic information

• Original name: Polymorphisms in genes encoding purinoreceptor and osteoprotegerin and external apical root resorption in children after orthodontic treatment
• Authors: VRANKOVÁ, Zuzana (703 Slovakia, belonging to the institution), Martina SIROTKOVÁ (703 Slovakia, belonging to the institution), Petra BOŘILOVÁ LINHARTOVÁ (203 Czech Republic, guarantor, belonging to the institution), Pavlína ČERNOCHOVÁ (203 Czech Republic, belonging to the institution), Jakub KAŠTOVSKÝ (203 Czech Republic, belonging to the institution) and Lydie IZAKOVIČOVÁ HOLLÁ (203 Czech Republic, belonging to the institution).
• Edition: 15th Euro Congress on Dental & Oral Health, Rome, 2016.

Other information

• Original language: English
• Type of outcome: Presentations at conferences
• Field of Study: Genetics and molecular biology
• Country of publisher: Italy
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14110/16:00088272
• Organization unit: Faculty of Medicine
• Doi: http://dx.doi.org/10.4172/2161-1122.C1.006
• Keywords in English: polymorphism; purinoreceptor; osteoprotegerin; external apical root resorption; orthodontic treatment
• Tags: EL OK

Abstract

Orthodontically induced external apical root resoption (EARR) is a multifactorial inflammatory disease. Genes encoding proteins, which are involved in the modulation of inflammatory response and bone remodeling, are considered as the “candidate” for EARR. The aim of this study was to analyze possible associations of single nucleotide polymorphisms (SNPs) in the P2RX7 (encoding purinoreceptor) and TNFRSF11B (encoding osteoprotegerin) genes with EARR in Czech children after orthodontic treatment. A total, 99 orthodontically treated patients (69 healthy and 30 with EARR) were enrolled in this case-control study. Genotype determination of P2RX7 +489C/T (rs208294, Tyr155His) and +1068C/T (rs1718119, Thr348Ala), TNFRSF11B -163C/T (rs3102735) and TNFRSF11B +1181C/G (rs2073618, Lys3Asn) was based on polymerase chain reaction using 5 nuclease TaqMan® assays. While no significant differences were observed in allele or genotype frequencies of all four studied SNPs, specific combinations of variants in P2RX7 may be associated with lower/higher risk of EARR development (P<0.05). In addition, the length of treatment by orthodontic appliances positively correlated with the presence of EARR (P<0.05). Although the effect of P2RX7 SNPs themselves to the development EARR was not confirmed in the Czech population, haplotype analysis suggests, that variability in the P2RX7 gene, as well as the length of treatment, may be important factors contributing to the etiopathogenesis of post-orthodontic EARR.

Links

• GB14-37368G, research and development project: Name: Centrum orofaciálního vývoje a regenerace

Investor: Czech Science Foundation

• MUNI/A/1258/2015, interní kód MU: Name: Analýza klinických, mikrobiálních a genetických faktorů v etiopatogenezi vybraných patologických stavů v dutině ústní

Investor: Masaryk University, Category A

• ROZV/24/LF/2016, interní kód MU: Name: LF - Příspěvek IP 2016

Investor: Ministry of Education, Youth and Sports of the CR