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@proceedings{1359060,
author = {Bencúrová, Petra and Baloun, Jiří and Musilová, Kateřina and Pospíšilová, Šárka and Hermanová, Markéta and Radová, Lenka and Pail, Martin and Brichtová, Eva and Mráz, Marek and Brázdil, Milan},
booktitle = {12th Annual microRNA as Biomarkers and Diagnostics},
keywords = {miRNA; epilepsy; hippocampal sclerosis; sequencing; qRT-PCR},
language = {eng},
title = {Whole miRNome Sequencing of Human Epileptic Brain Tissue},
url = {http://www.rnaconference.com/uploadedFiles/RNA_Conference/Agenda/16/2016-RNA-Conference-Brochure.pdf},
year = {2016}
}
TY - CONF
ID - 1359060
AU - Bencúrová, Petra - Baloun, Jiří - Musilová, Kateřina - Pospíšilová, Šárka - Hermanová, Markéta - Radová, Lenka - Pail, Martin - Brichtová, Eva - Mráz, Marek - Brázdil, Milan
PY - 2016
TI - Whole miRNome Sequencing of Human Epileptic Brain Tissue
KW - miRNA
KW - epilepsy
KW - hippocampal sclerosis
KW - sequencing
KW - qRT-PCR
UR - http://www.rnaconference.com/uploadedFiles/RNA_Conference/Agenda/16/2016-RNA-Conference-Brochure.pdf
L2 - http://www.rnaconference.com/uploadedFiles/RNA_Conference/Agenda/16/2016-RNA-Conference-Brochure.pdf
N2 - Mesial temporal lobe epilepsy (mTLE) is a chronic neurological disorder characterized by recurrent seizures. Accumulating evidence of microRNAs´ (miRNAs) potential involvement in pathogenic mechanisms underlying mTLE is emerging. The function of microRNAs in chronic mTLE is still poorly understood partially due to the lack of a clear cut miRNA expression profile of epileptic foci. In the present study, we performed the whole miRNome profiling analysis of the human brain tissue employing novel approach combining next generation sequencing (NGS) with an advanced qPCR technique, miQPCR. miRNA profiles of the hippocampus of autopsy controls and mTLE patients were compared, and analysis revealed differential miRNA expression of 24 miRNAs consistent with published data as well as several miRNA species never considered to play a role in epilepsy before. In conclusion, NGS exposed a group of miRNAs manifesting invariable expression across all samples out of which stable expression of four miRNAs was validated by combined methods for future utilization as negative controls or normalizers.
ER -
BENCÚROVÁ, Petra, Jiří BALOUN, Kateřina MUSILOVÁ, Šárka POSPÍŠILOVÁ, Markéta HERMANOVÁ, Lenka RADOVÁ, Martin PAIL, Eva BRICHTOVÁ, Marek MRÁZ a Milan BRÁZDIL. Whole miRNome Sequencing of Human Epileptic Brain Tissue. In \textit{12th Annual microRNA as Biomarkers and Diagnostics}. 2016.
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