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@article{1360245,
author = {Mlynáriková, Katarína and Šedo, Ondrej and Růžička, Filip and Zdráhal, Zbyněk and Holá, Veronika and Mahelová, Martina},
article_location = {Dordrecht},
article_number = {6},
doi = {http://dx.doi.org/10.1007/s12223-016-0458-7},
keywords = {FLIGHT MASS-SPECTROMETRY; DESORPTION IONIZATION-TIME; STAPHYLOCOCCUS-EPIDERMIDIS; RAMAN-SPECTROSCOPY; SURFACES; BACTERIA; IDENTIFICATION; ALBICANS; EXPRESSION; DATABASE},
language = {eng},
issn = {0015-5632},
journal = {Folia Microbiologica},
title = {Evaluation of capacity to detect ability to form biofilm in Candida parapsilosis sensu stricto strains by MALDI-TOF MS},
volume = {61},
year = {2016}
}
TY - JOUR
ID - 1360245
AU - Mlynáriková, Katarína - Šedo, Ondrej - Růžička, Filip - Zdráhal, Zbyněk - Holá, Veronika - Mahelová, Martina
PY - 2016
TI - Evaluation of capacity to detect ability to form biofilm in Candida parapsilosis sensu stricto strains by MALDI-TOF MS
JF - Folia Microbiologica
VL - 61
IS - 6
SP - 465-471
EP - 465-471
PB - Springer
SN - 00155632
KW - FLIGHT MASS-SPECTROMETRY
KW - DESORPTION IONIZATION-TIME
KW - STAPHYLOCOCCUS-EPIDERMIDIS
KW - RAMAN-SPECTROSCOPY
KW - SURFACES
KW - BACTERIA
KW - IDENTIFICATION
KW - ALBICANS
KW - EXPRESSION
KW - DATABASE
N2 - Matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is, currently, used as a rapid and reliable tool in microbial diagnostics. The discriminatory power of the method extends its applicability also beyond species level. This study examined the possibility to use MALDI-TOF MS to differentiate between Candida parapsilosis sensu stricto biofilm-positive (n = 12) and biofilm-negative (n = 9) strains. The results indicated a grouping trend within MALDI-TOF mass spectra belonging to each of the tested groups. However, these trends were eclipsed by mass spectral variations resulting from limited repeatability of the method, making its application for the selected purpose impossible. Improvement in the discriminatory power of the method was not obtained neither by using different matrices (alpha-cyano-4-hydroxycinnamic acid, ferulic acid, 5-chloro-2-mercaptobenzothionazole) for MALDI-TOF MS analysis nor by testing different culture conditions (cultivation length, culture media).
ER -
MLYNÁRIKOVÁ, Katarína, Ondrej ŠEDO, Filip RŮŽIČKA, Zbyněk ZDRÁHAL, Veronika HOLÁ and Martina MAHELOVÁ. Evaluation of capacity to detect ability to form biofilm in Candida parapsilosis sensu stricto strains by MALDI-TOF MS. \textit{Folia Microbiologica}. Dordrecht: Springer, 2016, vol.~61, No~6, p.~465-471. ISSN~0015-5632. Available from: https://dx.doi.org/10.1007/s12223-016-0458-7.
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